油桐花芽2个不同发育时期转录组分析

doi:10.11707/j.1001-7488.20140509

Abstract

Abstract:

This research compared transcriptome of Vernicia fordii flower buds at two different developing stages using RNA-seq technique and then obtained a lot of differentially expressed unigenes. Through GO classification and pathway enrichment analysis, all of these differentially expressed unigenes were classified into 55 GO terms and 128 metabolism pathways, respectively. One hundred and three unigenes identified from transcriptome data were referred to four major plant flowering regulation pathways including photoperiod pathway, verbalization pathway, GA-dependent pathway and autonomous pathway. With the development of flower buds, more and more unigenes' expression levels were up-regulated and the number of up-regulated genes was more than that of down-regulated genes. This research has resolved to some extent the molecular mechanisms regulating mode with flower bud morphological differentiation of V. fordii.

Key words: Vernicia fordii, flower bud, transcriptome sequencing, flowering genes

CLC Number:

S718.46

Sun Ying, Tan Xiaofeng, Luo Min, Li Jian. The Sequencing Analysis of Transcriptome of Vernicia fordii Flower Buds at Two Development Stages[J]. Scientia Silvae Sinicae, 2014, 50(5): 70-74.

References

曹建琦.1987. 谈谈桐油的β异构化.中国油脂,(6):34-37.
龚榜初,蔡金标. 1996. 油桐育种研究进展. 经济林研究,14(1):51-53.
黄福长. 2011. 国内外油桐发展现状.佛山科学技术学院学报: 自然科学版, 29(3):83-87.
(Huang F C. 2011. Current status of Vernicia fordii development at home and abroad. Journal of Foshan University(Natural Science Edition), 29(3):83-87. [in Chinese] )
黄坤,夏建陵. 2008. 桐油及其衍生物的改性在高分子材料中的应用进展. 化工进展,27(10): 1588-1592.
(Huang K, Xia J L. 2008. Progress of modification of tung oil and its derivatives in the application of polymer materials. Chemical Industry and Engineering Progress, 27(10): 1588-1592. [in Chinese] )
李建安,孙颖,陈鸿鹏,等.2008. 油桐LEAFY同源基因片段的克隆与分析. 中南林业科技大学学报,28(4): 21-26.
(Li J A, Sun Y, Chen H P, et al. 2008. Cloning and analysis of LEAFY homologous gene fragment from tung oil tree. Journal of Central South University of Forestry & Technology, 28(4): 21-26. [in Chinese] )
祁云霞,刘永斌,荣威恒.2011.转录组研究新技术: RNA-Seq及其应用. 遗传,33(11) : 1191-1202.
(Qi Y X, Liu Y B, Rong W H. 2011. RNA-Seq and its applications:a new technology for transcriptomics. Hereditas, 33(11) : 1191-1202. [in Chinese] )
孙昌辉,邓晓建,方军,等. 2007. 高等植物开花诱导研究进展. 遗传,29(10): 1182-1190.
(Sun C H, Deng X J, Fang J, et al. 2007. An overview of flowering transition in higher plants. Hereditas, 29(10): 1182-1190. [in Chinese] )
孙汉洲,杜勋军,谭晓风,等. 2010. 桐油制备生物柴油的工艺研究. 中南林业科技大学学报,30(12): 134-137.
(Sun H Z, Du X J, Tan X F, et al. 2010. Study on preparation of biodiesel from Tung oil. Journal of Central South University of Forestry & Technology, 30(12): 134-137. [in Chinese] )
孙颖,卢彰显,李建安. 2007. 中国油桐栽培利用与应用基础研究进展.经济林研究,25(2): 84-87.
(Sun Y, Lu Z X, Li J A. 2007. Literature review of researches on cultivation,utilization and basic application of tung oil tree. Nonwood Forest Research, 25(2): 84-87. [in Chinese] )
谭晓风. 2006. 油桐的生产现状及其发展建议. 经济林研究,24(3): 62-64.
(Tan X F. 2006. Status and suggestion on development of vernicia fordii. Nonwood Forest Research, 24(3): 62-64. [in Chinese] )
玄伟东,张天顺,张汝坤,等. 2007. 桐油制取生物柴油的研究.农业化研究,36(7): 204-206.
(Xuan W D, Zhang T S, Zhang R K, et al. 2007. The research about production of biodiesel from tung oil. Journal of Agricultural Mechanization Research, 36(7): 204-206. [in Chinese] )
张玲玲,彭俊华. 2011. 油桐资源价值及其开发利用前景. 经济林研究,29(3): 130-135.
(Zhang L L, Peng J H. 2011. Values,development and utilization prospect of Vernicia fordii resources. Nonwood Forest Research, 29(3): 130-135. [in Chinese] )
周燕君,陆向红,俞云良,等. 2008. 桐油制备生物柴油的研究. 能源工程, (5):33-35.
(Zhou Y J, Lu X H, Yu Y L, et al. 2008. Study of preparation of biodiesel with tung oil. Energy Engineering, (5):33-35. [in Chinese] )
朱世永,任庆生. 1992. 涂料工业中桐油利用的方向. 中国油脂, (S1): 390-398.
Amasino R M, Michaels S D. 2010. The timing of flowering. Plant Physiology, 154(2):516-520.
Brown K,Keeler W. 2005. The history of tung oil. Wildland Weeds, 9(1):4-24.
Chen Y H, Chen J H, Luo Y M. 2012. Complementary biodiesel combination from tung and medium-chain fatty acid oils. Renewable Energy, 44:305-310.
Hao D C,Ge G B,Xiao P G,et al.2011.The first insight into the tissue specific Taxus transcriptome via Illumina second generation sequencing. PLoS One,6(6) : e21220.
Hayama R,Yokio S, Tamaki S, et al. 2003. Adaptation of photoperiodic control pathways produces short-day flowering in rice. Nature, 422:719-722.
Izawa T,Oikawa T,Sugiyama N, et al. 2002. Phytochrome mediates the external light signal to repress FT orthologs in photoperiodic flowering of rice. Genes &Development,16(15): 2006-2020.
Jiang G X,Chen H,Tan X F. 2013. The expression analysis of genes in fatty acid biosynthesis pathway during the seed development of tung tree. Int J Bioautomation,17 (2): 73-82.
Kim D H, Doyle M R, Sung S, et al. 2009.Vernalization: winter and the timing of flowering in plants. Annual Review of Cell and Developmental, 25: 277-299.
Kojima S,Takahashi Y,Kobayashi Y,et al. 2002. Hd 3 a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd 1 under short-day conditions. Plant and Cell Physiology,43(10): 1096-1105.
Lee J, Lee I. 2010. Regulation and function of SOC 1 ,a flowering pathway integrator. Journal of Experimental Botany, 61(9):2247-2254.
Li D, Deng Z, Qin B, et al. 2012. De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree ( Hevea brasiliensis Muell). Arg BMC Genomics,13: 192.
Locasdo A, Lucchin M, Varollo S. 2009. Characterization of a MADS FLOWERING LOCUSC-LIKE (MFL) sequence in Cichorium intybus: a comparative study of CiMFL and AtFLC reveals homologies and divergences in gene function. New Phytologisl,182(3):630-643.
Samach A, Onouchi H, Gold S E, et al. 2000. Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science, 288(5471): 1613-1616.
Shang Q, Jiang W, Lu H, et al. 2002. Properties of tung oil biodiesel and of methylsoyate. Journal of the American Oil Chemists Society, 79(9):915-920.
Shi C Y, Yang H, Wei C L, et al. 2011. Deep sequencing of the Camellia sinensis transcriptome revealed candidate genes for major metabolic pathways of tea-specific compounds. BMC Genomics,12: 131
Steven P. 2010. Determining biological roles of four unique Vernicia fordii acyl-CoA binging proteins. Louisiana: University of New Orleans.
Suarez L P, Wheatley K, Robson F, et al. 2001. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature, 410(6832): 1116-1120.
Tuskan G A,Difazio S,Jansson S,et al. 2006. The genome of black cottonwood,Populus trichocarpa (Torr. & Gray). Science, 313:1596-1604.
Wilhelm B T, Landry J R. 2009. RNA-Seq-quantitative measurement of expression through massively parallel RNA-sequencing. Methods, 48(3):249-257.

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The Sequencing Analysis of Transcriptome of Vernicia fordii Flower Buds at Two Development Stages

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