Abstract:

Sophora japonica is a valuable gardening tree that has been planted in the North of China. However, a problem hampering the application of its full potential is susceptibility to insect. In this study, taking leaves of Sophora japonica as the explants, the optimum medium for the induction of adventitious bud differentiation was selected. Modified cowpea trypsin inhinitor(CpTI)gene sck(Signal-CpTI-KDEL), an insecticidal gene, was introduced into S. japonica by Agrobacterium-mediated genetic transformation. After cocultivation of leaves of S. japonica with Agrobacterium tumefaciens harbored sck gene, kanamycin resistance shoots were transferred to selective MS medium containing 3 mg·L^-1 BA, 0.05 mg·L^-1IAA, 8 mg·L^-1 G418 and 500 mg·L^-1 cefotaxime. Transgenic plants were obtained after rooting on half-strength MS medium supplemented with 1.0 mg·L^-1 indole-3-butyrique acide (IBA), 10 mg·L^-1 G418 and 100 mg·L^-1 cefotaxime. PCR and Southern blotting confirmed that Modified CpTI gene(sck)was successfully transferred into S. japonica mediated by A. tumefaciens.

Key words: Sophora japonica, Agrobacterium tumefaciens, cowpea trypsin inhinitor(CpTI) gene sck, gene transformation