农杆菌介导BADH基因转化美丽胡枝子的研究

doi:10.11707/j.1001-7488.20080317

Scientia Silvae Sinicae ›› 2008, Vol. 44 ›› Issue (3): 76-80.doi: 10.11707/j.1001-7488.20080317

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Transformation of Lespedeza formosa with BADH Gene Mediated by Agrobacterium tumefaciens

Hu Dongnan^1,2,Chen Xiaoyang²,Li Wei²,Pian Ruiqi²,Zhao Yang³

1.College of Landscape Architecture and Art,Jiangxi Agricultural University Nanchang 330045; 2.Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education Beijing Forestry University Beijing 100083; 3. Forestry College of Guizhou University Guiyang 550025

Received:2007-01-25 Revised:1900-01-01 Online:2008-03-25 Published:2008-03-25

Abstract

Abstract: The genetic transformation of Lespedeza formosa mediated by Agrobacterium tumefaciens was studied by using the cotyledonary nodes as explants and the Glufosinate(Glu) as a selector.The genetic transformation system of Lespedeza formosa was developed and the BADH transgenic plants were achieved. The highest transformation frequency was able to reach to 28%. The molecular analysis showed that the BADH gene was integrated into genome of L.formosa. The transgenic plants were able to accumulate more betaine than the untransformed ones under NaCl stress,which demonstrated the transgenic plants were able to express BADH gene normally.

Key words: Lespedeza formosa, BADH gene, gene transformation, betaine

Hu Dongnan;Chen Xiaoyang;Li Wei;Pian Ruiqi;Zhao Yang. Transformation of Lespedeza formosa with BADH Gene Mediated by Agrobacterium tumefaciens[J]. Scientia Silvae Sinicae, 2008, 44(3): 76-80.

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Transformation of Lespedeza formosa with BADH Gene Mediated by Agrobacterium tumefaciens

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