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Scientia Silvae Sinicae ›› 2002, Vol. 38 ›› Issue (6): 30-35.doi: 10.11707/j.1001-7488.20020606

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STUDIES ON TRANSFORMATION OF MTLD/GUTD DIVALENT GENES TO POPULUS DELTOIDS×P. CATHAYANA

Fan Junfeng,Han Yifan,Li Ling,Peng Xuexian,Li Jiarui   

  1. College of Forestry, Northwest Sci-Tech University of Agriculture and Forestry Yangling712100;Forestry Research Institute, Chinese Academy of Forestry Beijing100091;Institute of Microbiology, Academia Sinica Beijing100080;College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry Yangling712100
  • Received:2002-07-09 Revised:1900-01-01 Online:2002-11-25 Published:2002-11-25

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Abstract:

Transformation of mtlD/gutD divalent genes to Populus deltoids × P.cathayana by means of leaf disc was undertaken. Through successive selection in shoot and root induction stage under high level kanamycin pressure, 38 kanamycin-resistant rooted plants were obtained. PCR analysis showed that 28 of these kanamycin-resistant rooted plants were positive. 5 of these 28 PCR positive plants were taken out for Southern blot and Western blot, and the result indicated that 4 of the 5 plants were positive for both. This meant that mtlD/gutD divalent genes were successfully integrated into the genome of these 4 plants, and the proteins expressed by mtlD/gutD divalent genes were synthesized. The salt resistant test demonstrated that salt tolerance ability of these 4 plants was enhanced to some extent.

Key words: Populus deltoids ×, P. cathayana;MtlD/gutD divalent genes;Gene transformation;Molecular biological analysis;Salt resistant test