Welcome to visit Scientia Silvae Sinicae，Today is

Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (11): 60-66.doi: 10.11707/j.1001-7488.20131108

Previous Articles     Next Articles

Transformation of BG2 Gene into Populus×euramericana cv.‘Neva’and Resistance of the Transgenic Poplar to Canker Disease

Niu Qinglin1, Wang Ying2, Luo Lei2, Huang Yanyan2, Liu Jing2, Feng Dianqi1,2, Cao Banghua1   

  1. 1. Key Laboratory of Agricultural Ecology and Environment, Shandong Agricultural University Tai'an 271018;
    2. Taishan Institute of Forestry Science Tai'an 271000
  • Received:2013-06-06 Revised:2013-08-08 Online:2013-11-25 Published:2013-11-26

PDF (PC)

920

Abstract:

The BG2 gene was transformed into Populus×euramericana cv.‘Neva’via Agrobacterium tumefaciens mediated transformation method. Four transgenic lines were obtained through selection of kanamycin resistance, PCR and Southern analyses. These analyses suggested that the BG2 gene was integrated into the plant genome. Then, the transgenic plants were tested for resistance to the fungal pathogen Cytospora chrysosperma. Crude extracts of the transgenic T-107 plants exhibited in vitro inhibitory activity against the fungal pathogen. When inoculated with Populus×euramericana canker disease, the T-107 plants grew better than the C-107 plants. The MDA levels of T-107 were lower than that in C-107. The PAL contents of both transgenic and control plants were firstly increased obviously then decreased; However, the PAL content of T-107 plants was always higher than that in C-107 plants. After 15 days, all the C-107 plants dead, but the T-107 was still alive. These results indicated that the transformed BG2 gene could improve the resistance of T-107 plants to canker disease.

Key words: Populus×, euramericana cv.‘Neva’, Agrobacterium tumefaciens, BG2 gene, gene transformation, canker disease

CLC Number: