Abstract:

Newly grown branch buds on node of Dendrocalamus latiflorus were used as explants to induce buds in MS, 1/2 MS, and N₆ media with different hormone combinations, and tube seedlings were obtained. In order to improve its reproductive coefficient, survival rate and rooting morphology, four aspects were tested: the number of the inoculated seedlings per cluster, multiplication culture, rooting culture and field transplanting. The results showed that the optimum medium for primary culture was MS+BA2.0 mg·L^{-1+IBA 0.5 mg·L-1; the reproductive coefficient reached 7.31 with sturdy seedlings during multiplication and rooting culture, if four seedlings were inoculated per cluster in MS+ BA2.0 mg·L-1 + IBA0.3 mg·L-1 medium; the optimal medium for rooting was 1/2MS+ IBA4.0 mg·L-1, with survival rate as high as 95%. The rooted seedlings were transplanted in the medium consisting of perlite+ earth+ cinders ash (1∶1∶1); After 3 to 7 days of hardening in the bottle and then growing for 10 to 15 days, 90% of the seedlings were survived. Through the above method, more than 2 million bamboo seedlings have been propagated so far.}

Key words: Dendrocalamus latiflorus, tissue culture, rapid propagation in factory