Abstract:

Somatic embryogenesis and regeneration plantlets were successfully induced from leaves of Euphorbia cotinifolia by tissue culture. The highest callus induction rate was 57.2% on MS medium with 6-BA 1.0 mg·L^-1,NAA 0.5 mg·L^-1and 2,4-D 0.01 mg·L^-1. Embryonic callus could be induced on MS medium with ZT 1.0 mg·L^-1, 2,4-D 0.5 mg·L^-1and NAA 0.05 mg·L^-1after 3 times of subculture of callus, and the induction rate was 56.4%. Somatic embryogenesis was induced on MS medium with ZT 1.0 mg·L^-1and NAA 0.1 mg·L^-1,and the induction rate could be up to 35.4%.The regeneration plantlets and somatic embryogenesis were rooted on MS medium without hormones. The plantlets with root were successfully transplanted to the medium with vermiculite and ruby mica and sands by 3 to 2 to 1 in volume, and the survival rate could be up to 83.3%.

Key words: Euphorbia cotinifolia, leaf, tissue culture, somatic embryogenesis