Abstract:

Young stem section with buds of cutting plantlets of Taxus media ‘Hicksii’ was used as explants for armpit bud multiplication plantlet regeneration experiment. The results showed, MS+6-BA 0.05 mg·L^-1 +NAA 0.5 mg·L^-1 is the optimal culture medium for startup culture, the startup rate is 89.55%. 6-BA is indispensable for startup, but the concentration of it should be low. The growth regulators were beneficial for the startup of the buds, but harmful for the growth of it. MS (double organic matter) culture medium with no growth regulators was suitable for buds to grow on. The valid buds rate is 72.73%; mean length of buds is 3.3 cm. The induction of adventitious roots needed to establish a suitable balance of auxin and cytokinin in the plantlet. Retting cuttings in IBA liquor (1 000 mg·L^-1) 30 s was a fit means for root induction; the rooting rate is 58.33%.

Key words: Taxus media &lsquo, Hecksii&rsquo, tissue culture, armpit bud multiplication, plantlet regeneration