Abstract: Bud induction and fast propagation were carried out by using shoot segments containing auxiliary buds of Corylus heterophylla as primary materials. The results showed that, primary medium DKW+5 mg·L^-1 6-BA+0.01 mg·L^-1 IBA supplemented with 2.0 g·L^-1 vitamin C was profitable to prevent browning of explants, and addition of 64.42 mg·L^-1 putrescine,58.10 mg·L^-1 spermidine and 20.20 mg·L^-1 spermine promoted bud germination rate and growth speed. Calli or buds occurred from the cuts of shoot segments on the primary medium. The calli were transferred to medium DKW+0.01 mg·L^-1 IBA+2.5 mg·L^-1 TDZ+32.21 mg·L^-1 putrescine+29.05 mg·L^-1 spermidine+ 10.10 mg·L^-1 spermine and induced to produce adventitious buds. The treatments with polyamines were profitable to promote buds germination and growth. Over 90% regeneration plantlets were able to root after transferred to medium 1/2MS + IBA 0.25 mg·L^-1 .

Key words: Corylus heterophylla, tissue culture, rapid propagation