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Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (2): 51-55.doi: 10.11707/j.1001-7488.20100209

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Transformation of the Insecticidal Agglutinin Gene(gna) from Snowdrop (Galanthus nivalis) into Sophora japonica and Resistance of the Transgenic Plants to Aphids

Zhang Xiaoying1,Gan Jing2,Yin Weilun3,Zhu Zhen4,Wang Huafang3   

  1. 1.Beijing Langshan Nursery Beijing 100039; 2.Beijing Gardening and Greening Bureau Beijing 100029; 3.College of Biological Science and Biotechnology,Beijing Forestry University Beijing 100083; 4.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101
  • Received:2008-09-18 Revised:1900-01-01 Online:2010-02-25 Published:2010-02-25

Abstract:

Snowdrop (Galanthus nivalis) agglutinin (GNA) is toxic to sap sucking injurious insects of Homopteran. In this study,gna gene was successfully transformed into Sophora japonica by using the Agrobacterium tumefaciens. Transgenic plants have been obtained. Kanamycin resistant test, PCR and Southern blot bioassays confirmed that gna gene was integrated into Sophora japonica genome DNA. The test about expression of gna inhibitory activity indicated that almost every transgenic plant exhibited more or less stronger ability to agglomerate red-blood cell of sheep than the non-transformed plants. Some transgenic plants were observed to have stronger resistance to aphids than the control plants in the laboratory test.

Key words: Sophora japonica, Snowdrop (Galanthus nivalis) agglutinin (GNA), Aphis medicaginis, Agrobacterium tumefaciens, genetic transformation, insect density