Apl 基因转化地被菊品种‘玉人面’的研究*

doi:10.11707/j.1001-7488.20070923

Scientia Silvae Sinicae ›› 2007, Vol. 43 ›› Issue (09): 128-132.doi: 10.11707/j.1001-7488.20070923

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Genetic Transformation of Chrysanthemum morifolium cv．‘Yu Ren Mian’with AP1 Gene Mediated by Agrobacterium tumefaciens

Lü Jinhui^1,2,Wu Yueliang¹,Sun Lei¹,Zhang Qixiang¹

1．College of Landscape Architecture，Beijing Forestry Univenity Beijing 100083；2．College of Forest，Shanxi Agricultural University raigu 030801

Received:2006-09-19 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

Abstract

Abstract:

The flower’s meristematic characteristic gene Apl was introduced into Chrysanthemum morifolium cv．‘Yu RenMian’mediated by Agrobacterium tumefaciens．The factors influencing genetic transformation protocol were studied．The resuhsshowed that the leaf explants precultured for 2～8 hours or not precuhured were best for transformation by A．Tumefaciens．The suitable concentration of bacterial and the time for infecting was OD₆₀₀ 0.5 for 10 minutes．Leaves were cocultivated with bacterial at 23～25℃ for 2 days．Then delayed selection for 3 days．Kan^r plant were selected by increased selective press from 5 mg·L^-1 G418 to 7.5 mg·L^-1 G418 followed by 10 mg·^-1 G418．The integration of Apl gene into C．Morifolium‘Yu Ren Mian’was confirmed by PCR and Southern blotting．Two of transgenic plants bloomed 15 days earlier than untransformed plants．

Key words: Chrysanthemum morifolium, Apl gene, Agrobacterium tumefaciens, genetic transformation

Lü Jinhui;Wu Yueliang;Sun Lei;Zhang Qixiang. Genetic Transformation of Chrysanthemum morifolium cv．‘Yu Ren Mian’with AP1 Gene Mediated by Agrobacterium tumefaciens[J]. Scientia Silvae Sinicae, 2007, 43(09): 128-132.

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Genetic Transformation of Chrysanthemum morifolium cv．‘Yu Ren Mian’with AP1 Gene Mediated by Agrobacterium tumefaciens

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