香樟胚性愈伤组织遗传转化体系建立*

doi:10.11707/j.1001-7488.20080411

Scientia Silvae Sinicae ›› 2008, Vol. 44 ›› Issue (4): 54-59.doi: 10.11707/j.1001-7488.20080411

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Establishment of Agrobacterium Mediated Transformation System of Embryogenic Calli of Cinnamomum camphora

Du Li¹,Pang Zhenling¹,Zhou Suo¹,Zeng Xiaohui¹,Bao Manzhu²

(1. Shool of Life Science and Technology, Nanyang Normal University Nanyang 473000; 2. Key Laboratory of Horticultural Plant Biology of Ministry of Education College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan 430070)

Received:2007-09-19 Revised:1900-01-01 Online:2008-04-25 Published:2008-04-25

Abstract

Abstract:

A genetic transformation system of embryogenic calli (Cinnamomum camphora) mediated by Agrobacterium tumefaciens was established. Experimental result indicated that efficient transformation was obtained with embryogenic calli as acceptor. Hygromycin of 50 mg\5L^-1 was efficient in screening embryogenic calli. When the OD600 value of Agrobacterium was 0.6, a high transformation rate was gained. The infection time of 40 min, combined with co culture time of 3 d, remarkably enhanced transformation rate. Results of PCR proved that the GUS gene had integrated into embryogenic calli of C. camphora genome.

Key words: Cinnamomum camphora, Agrobacterium tumefaciens, genetic transformation, PCR

Du Li;Pang Zhenling;Zhou Suo;Zeng Xiaohui;Bao Manzhu. Establishment of Agrobacterium Mediated Transformation System of Embryogenic Calli of Cinnamomum camphora[J]. Scientia Silvae Sinicae, 2008, 44(4): 54-59.

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Establishment of Agrobacterium Mediated Transformation System of Embryogenic Calli of Cinnamomum camphora

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