南林 895 杨抗旱耐盐基因 DREB1C 的转化

doi:10.11707/j.1001-7488.20090203

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (2): 17-21.doi: 10.11707/j.1001-7488.20090203

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Transformation of Drought and Salt Resistant Gene ( DREB1C ) in Populus×euramericana cv. Nanlin 895

Yang Chunxia^1,2,Li Huogen¹,Cheng Qiang¹,Chen Ying¹

(1. Key Laboratory of Genetics & Biotechnology of Ministry of Education Nanjing Forestry University Nanjing 210037; 2. Jiangxi Academy of Forestry Nanchang 330032)

Received:2008-01-21 Revised:1900-01-01 Online:2009-02-25 Published:2009-02-25

Abstract

Abstract:

Abstract: An elite hybrid clone of Populus×euramericana cv. Nanlin 895 was used as the receptor material for transgenic transformation, and Agrobacteriummediated method was applied to introduce DREB1C gene encoding a transcription factor related with stresses.A total 80 resistant plants were gained via strictly selection by hygromycin. Among them 30 positive transgenic plants were verified by PCR and real-time PCR analysis. It is indicated that DREB1C gene was probably integrated into genome of Nanlin 895. Furthermore, results of resistant experiment showed that resistance of transgenic plants to drought and salt was stronger than non-transgenic plants.

Key words: Key words: Populus×, euramericana cv. Nanlin 895, DREB1C gene, genetic transformation, drought and salt resistance

Yang Chunxia;Li Huogen;Cheng Qiang;Chen Ying . Transformation of Drought and Salt Resistant Gene ( DREB1C ) in Populus×euramericana cv. Nanlin 895[J]. Scientia Silvae Sinicae, 2009, 12(2): 17-21.

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Transformation of Drought and Salt Resistant Gene ( DREB1C ) in Populus×euramericana cv. Nanlin 895

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