国槐转雪花莲凝集素基因及抗蚜性

doi:10.11707/j.1001-7488.20100209

Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (2): 51-55.doi: 10.11707/j.1001-7488.20100209

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Transformation of the Insecticidal Agglutinin Gene(gna) from Snowdrop (Galanthus nivalis) into Sophora japonica and Resistance of the Transgenic Plants to Aphids

Zhang Xiaoying¹,Gan Jing²,Yin Weilun³,Zhu Zhen⁴,Wang Huafang³

1.Beijing Langshan Nursery Beijing 100039; 2.Beijing Gardening and Greening Bureau Beijing 100029; 3.College of Biological Science and Biotechnology，Beijing Forestry University Beijing 100083; 4.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101

Received:2008-09-18 Revised:1900-01-01 Online:2010-02-25 Published:2010-02-25

Abstract

Abstract:

Snowdrop (Galanthus nivalis) agglutinin (GNA) is toxic to sap sucking injurious insects of Homopteran． In this study,gna gene was successfully transformed into Sophora japonica by using the Agrobacterium tumefaciens． Transgenic plants have been obtained． Kanamycin resistant test， PCR and Southern blot bioassays confirmed that gna gene was integrated into Sophora japonica genome DNA． The test about expression of gna inhibitory activity indicated that almost every transgenic plant exhibited more or less stronger ability to agglomerate red-blood cell of sheep than the non-transformed plants． Some transgenic plants were observed to have stronger resistance to aphids than the control plants in the laboratory test．

Key words: Sophora japonica, Snowdrop (Galanthus nivalis) agglutinin (GNA), Aphis medicaginis, Agrobacterium tumefaciens, genetic transformation, insect density

Zhang Xiaoying;Gan Jing;Yin Weilun;Zhu Zhen;Wang Huafang. Transformation of the Insecticidal Agglutinin Gene(gna) from Snowdrop (Galanthus nivalis) into Sophora japonica and Resistance of the Transgenic Plants to Aphids[J]. Scientia Silvae Sinicae, 2010, 46(2): 51-55.

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Transformation of the Insecticidal Agglutinin Gene(gna) from Snowdrop (Galanthus nivalis) into Sophora japonica and Resistance of the Transgenic Plants to Aphids

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