模拟运输下减振包装对猕猴桃货架品质和苹果酸代谢的影响

doi:10.11707/j.1001-7488.LYKX20240052

Abstract

Abstract:

Objective: This study aims to investigate the effects of different anti-vibration packaging materials on the shelf-life quality of kiwifruit during the storage and transportation of ‘Xu Xiang’ kiwifruit, as well as the effects of expanded polyethylene (EPE) packaging treatment on the activity of relevant enzymes and gene expression during the malic acid metabolism of kiwifruit after harvest. Method: Three kinds of anti-vibration packaging treatments, namely, foam mesh bag packaging, expanded polystyrene (EPS) and expanded polyethylene (EPE), were adopted, and road transportation was simulated in a 72 h vibration experiment on a vibration test bench. The shelf-life qualities of kiwifruit (hardness, color, and the contents of titratable acid, soluble solids, vitamin C and total phenol) were determined. Then, the indicators of cell membrane damage (malondialdehyde, relative conductivity and lipoxygenase activity) were analyzed, and the activity and gene expression of enzymes involved in malic acid metabolism were measured and the correlation among the measured data was investigated. Result: Compared with the foam mesh bag and EPS packaging, the EPE packaging maintained the hardness and color of kiwifruit fruits, promoted the synthesis of saccharides, and inhibited the decomposition of antioxidants. It also slowed down the rise of relative conductivity and malondialdehyde content, reduced lipid peroxidation and maintained the integrity of cell membranes. Furthermore, results showed that malic acid content was positively correlated with synthesis-related NAD-MDH and PEPC enzyme activities, and negatively correlated with degradation-related NADP-ME enzyme activities. EPE packaging stabilized the malic acid metabolism-related enzyme activities and gene expression. Conclusion: EPE anti-vibration packaging treatment is beneficial to the maintenance of shelf-life quality of kiwifruit fruit during storage and transportation. It exhibits a positive effect on malic acid metabolism of kiwifruit fruits.

Key words: Kiwifruit, simulated transportation, fruit quality, anti-vibration packaging, malic acid metabolism, gene expression

CLC Number:

TS255.3

Zihao Zong,Ruiling Liu,Weijie Wu,Huizhi Chen,Chuan Tong,Shuren Han,Zhengshi Jin,Xianming Ye,Haiyan Gao. Effects of Anti-vibration Packaging on Shelf-life Quality and Malic Acid Metabolism of Kiwifruit under Simulated Transportation[J]. Scientia Silvae Sinicae, 2025, 61(2): 122-130.

Figures/Tables 6

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基因 Gene	引物名称Primer name	引物序列（5’→3’） Primer sequence (5'→3')	GC含量 GC content（%）
PEPC	PEPC-F	ACGGAAGGATACGGAATTGC	50.0
	PEPC-R	CCGTAACGCAGCTTGAATCT	50.0
NADP-ME	NADP-ME-F	CATGATGAAATGCTTCTGGC	45.0
	NADP-ME-R	GGCGGATAGATCAAACACTT	45.0
NAD-MDH	NAD-MDH-F	GCTGCTGATGAATGGTCTTG	50.0
	NAD-MDH-R	GGAGTATGCCAGAGCCTTT	52.6

组别 Groups	指标 Indices	苹果酸含量 Malic acid content	NADP-ME酶活 NADP-ME enzyme activity	NAD-MDH酶活 NAD-MDH enzyme activity	PEPC酶活 PEPC enzyme activity
EPE缓冲包装 EPE Cushion Packaging	苹果酸含量Malic acid content	1
	NADP-ME酶活NADP-ME enzyme activity	?0.897**	1
	NAD-MDH酶活NAD-MDH enzyme activity	0.803**	?0.838**	1
	PEPC酶活PEPC enzyme activity	0.673**	?0.736**	0.865**	1
	NADP-ME基因NADP-ME gene expression	0.182	0.134	0.033	0.060
	NAD-MDH基因NAD-MDH gene expression	0.598**	?0.499**	0.678**	0.810**
	PEPC基因PEPC gene expression	0.807**	?0.845**	0.859**	0.898**
纸箱包装 Cardboard packaging	苹果酸含量Malic acid content	1
	NADP-ME酶活NADP-ME enzyme activity	?0.402	1
	NAD-MDH酶活NAD-MDH enzyme activity	?0.183	?0.349	1
	PEPC酶活PEPC enzyme activity	?0.191	?0.352	0.943**	1
	NADP-ME基因NADP-ME gene expression	?0.506*	0.644**	?0.072	?0.134
	NAD-MDH基因NAD-MDH gene expression	0.320	?0.202	0.378	0.371
	PEPC基因PEPC gene expression	?0.367	0.308	0.640*	0.568*

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Effects of Anti-vibration Packaging on Shelf-life Quality and Malic Acid Metabolism of Kiwifruit under Simulated Transportation

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