Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (3): 108-116.doi: 10.11707/j.1001-7488.20210311
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Xinyi Zhou,Liqiong Yan,Yuntong Lü,Lili Sun,Jingwen Zhu,Chuanwang Cao*
Received:
2019-12-24
Online:
2021-03-01
Published:
2021-04-07
Contact:
Chuanwang Cao
CLC Number:
Xinyi Zhou,Liqiong Yan,Yuntong Lü,Lili Sun,Jingwen Zhu,Chuanwang Cao. Activities and Gene Expressions of Phenylpropane Metabolic Enzymes in Populus simonii×P. nigra by Herbivore Induction of Lymantria dispar (Lepidoptera: Lymantriidae)[J]. Scientia Silvae Sinicae, 2021, 57(3): 108-116.
Table 1
Primer sequences for real-time RT-PCR"
基因 Gene | 正向引物序列 Forward primer sequence (5′-3′) | 反向引物序列 Reverse primer sequence (5′-3′) |
C4H-1 | CGACCCTGAACTAGCCAACC | GGTCATCGACCACAAGGTCC |
C4H-2 | TGGGGAATTGCTGAGCTTG | TGCTCTCAGCTGGAATGTCG |
4CL | GGCGATGTAATCATGACCCTGC | CTCTGGTGGGTCATCAATGGTG |
EF1β | AAGAGGACAAGAAGGCAGCA | CTAACCGCCTTCTCCAACAC |
UBQ | GTTGATTTTTGCTGGGAAGC | GATCTTGGCCTTCACGTTGT |
Table 2
Characteristics of C4H and 4CL genes in P. simonii × P. nigra"
基因 Gene | 开放阅读框 Open reading frame/bp | 编码氨基酸 Encoding amino acids | 分子质量 Molecular mass/kDa | 理论等电点 Theoretical pI | 含量最多的氨基酸 The most abundant amino acids |
C4H-1 | 1 614 | 537 | 61.40 | 8.86 | Leu |
C4H-2 | 1 518 | 505 | 58.00 | 9.09 | Leu |
4CL | 1 740 | 579 | 63.03 | 5.63 | Leu |
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