Scientia Silvae Sinicae ›› 2023, Vol. 59 ›› Issue (12): 117-124.doi: 10.11707/j.1001-7488.LYKX20220253
• Research papers • Previous Articles Next Articles
Ming'ao Cao1,Fei Zhang2,Guangming Huang1,Ruicheng Liu1,Liping Liu1,Qiangsheng Wu1,*,Yongjie Xu3
Received:
2022-04-16
Online:
2023-12-25
Published:
2024-01-08
Contact:
Qiangsheng Wu
CLC Number:
Ming'ao Cao,Fei Zhang,Guangming Huang,Ruicheng Liu,Liping Liu,Qiangsheng Wu,Yongjie Xu. Effects of Arbuscular Mycorrhizal Fungi on Phosphorus Uptake of Walnut Seedling Roots under Low Phosphorus Stress and the Potential Mechanisms[J]. Scientia Silvae Sinicae, 2023, 59(12): 117-124.
Table 1
The primer sequence of genes in qRT-PCR"
基因 Genes | 登记号 Accessions | 引物序列 Primer sequence(5’→3’) |
JrPAP10 | Jm3DG00060700 | F: ACACCCCTCAATACCAATGGC |
R: ACTCTCATGGTTTCCCCCTCC | ||
JrPAP12 | Jm4DG00150700 | F: ACACTCCACAATACAAATGGC |
R: TGGGCAATGCATAAAAACGAT | ||
JrPHT3;1 | Jm2DG00002500 | F: ATCTCATCTGGGTTTGGAGTG |
R: CTGAGCACTGTAGCCAAGCAA | ||
JrPHT3;2 | Jm6DG00001600 | F: TGCCCACTTTTCTAGGTTACA |
R: AGGTTTTGTACTTGGTTGCAT | ||
18S rRNA | LOC109010173 | F: GGTCAATCTTCTCGTTCCCTT |
R: TCGCATTTCGCTACGTTCTT |
Table 2
Effects of D. spurca on biomass of walnuts seedlings under low P stress"
AM真菌接种 AM fungal inoculation | P处理 P treatment | 地上部生物量 Shoot biomass/(g·plant?1) | 地下部生物量 Root biomass/(g·plant?1) | 总生物量 Total biomass/(g·plant?1) |
?A | ?P | 7.74±0.47d | 9.05±0.69d | 18.11±1.39d |
+P | 9.86±0.52c | 12.03±1.25c | 24.07±2.51c | |
+A | ?P | 12.11±1.08b | 13.72±0.42b | 27.43±0.84b |
+P | 15.43±1.02a | 15.32±1.13a | 30.63±2.27a | |
显著性 Significance | ||||
A | 0.0001 | <0.0001 | <0.0001 | |
P | 0.0001 | 0.0004 | 0.0005 | |
A×P | 0.1688 | 0.1665 | 0.1455 |
Table 3
Effects of D. spurca on root morphology of walnut seedlings under low P stress"
AM真菌接种 AM fungal inoculation | P处理 P treatment | 总长 Total length/cm | 投影面积 Projected area/cm2 | 表面积 Surface area/cm2 | 平均直径 Average diameter/mm | 体积 Volume/cm3 |
?A | ?P | 600±65b | 47.6±4.3b | 149±16b | 0.73±0.04c | 3.05±0.16b |
+P | 609±70b | 58.2±6.7a | 167±15ab | 0.97±0.06a | 3.09±0.25b | |
+A | ?P | 728±65a | 60.3±4.0a | 190±13a | 0.94±0.09ab | 4.42±0.25a |
+P | 645±38ab | 53.1±7.3ab | 183±21a | 0.85±0.09b | 4.57±0.39a | |
显著性 Significance | ||||||
A | 0.0199 | 0.2068 | 0.0047 | 0.2377 | <0.0001 | |
P | 0.2489 | 0.5673 | 0.5240 | 0.0775 | 0.4998 | |
A×P | 0.1560 | 0.0091 | 0.1622 | 0.0007 | 0.7064 |
Fig.2
Effects of D. spurca on root P content (a) and acid phosphatase activity (b) of walnut seedlings under low P stress –A–P: Non-inoculation with AM fungi under low P; –A+P: Non-inoculation with AM fungi under appropriate P; +A–P: Inoculation with AM fungi under low P; +A+P: Inoculation with AM fungi under appropriate P."
Table 4
Variable significance of the interaction between AM fungal inoculation (A) and P treatments (P)"
变量 Variables | A | P | A×P | 变量 Variables | A | P | A×P | |
土壤酸性磷酸酶Soil acid phosphatase | <0.0001 | 0.7781 | 0.0061 | JrPT3;1 | <0.0001 | 0.0002 | 0.0002 | |
土壤中性磷酸酶Soil acid phosphatase | <0.0001 | <0.0001 | <0.0001 | JrPT3;2 | 0.0002 | <0.0001 | 0.0062 | |
土壤碱性磷酸酶Soil alkaline phosphatase | <0.0001 | <0.0001 | <0.0001 | JrPAP10 | <0.0001 | <0.0001 | <0.0001 | |
根系酸性磷酸酶Root acid phosphatase | 0.0189 | 0.0350 | 0.2499 | JrPAP12 | 0.7294 | 0.0256 | 0.0095 | |
根系P Root P | <0.0001 | <0.0001 | <0.0001 |
Fig.4
Effect of D. spurca on the relative expression of acid phosphatase secretion genes (a) and phosphate transporter protein genes (b) of walnut roots under P stress –A–P: Non-inoculation with AM fungi under low P; –A+P: Non-inoculation with AM fungi under appropriate P; +A–P: Inoculation with AM fungi under low P; +A+P: Inoculation with AM fungi under appropriate P."
Table 5
Relationship between P content and relevant variables of walnut roots"
项目 | 土壤磷酸酶 Soil acid phosphatase | 根系酸性 磷酸酶 Root acid phosphatase | 根系P Root P | JrPT3;1 | JrPT3;2 | JrPAP10 | JrPAP12 | 根系性状 Root character | ||||||
酸性 Acid | 中性 Neutral | 碱性 Alkaline | 根长 Root length | 投影面积 Projected area | 表面积 Surface area | 平均直径 Diameter | 体积 Volume | |||||||
根系P Root P | 0.57 | 0.40 | 0.67* | 0.11 | 1.00 | 0.27 | ?0.93** | 0.13 | 0.66* | 0.22 | 0.58* | 0.59* | 0.61* | 0.49 |
菌根侵染率 Mycorrhizal colonization | 0.84** | 0.90** | 0.77** | 0.51 | 0.45 | 0.85** | ?0.48 | 0.71** | 0.10 | 0.60* | 0.33 | 0.67** | 0.23 | 0.91** |
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