桉树生长和防御相关酶对摩西管柄囊霉和青枯菌的响应

doi:10.11707/j.1001-7488.LYKX20220095

Abstract

Abstract:

Objective: This study aims to investigate the growth and physiological characteristics of Eucalyptus grandis in response to Funneliformis mosseae and Ralstonia solanacearum, so as to provide some theoretical support for application mycorrhizal technology to prevent and control Eucalyptus bacterial wilt disease. Method: E. grandis seedlings were used to study the effects of F. mosseae mycorrhizal during the infection course of R. solanacearumon the host plant growth and defense-related enzyme activities. Result: 1) F. mosseae was well symbiotic with the root of E. grandis. The plant height, ground diameter, dry weight and root-shoot ratio of mycorrhizal Eucalyptus were 2.30, 4.38, 2.75 and 1.71 times higher than those of non-mycorrhizal Eucalyptus, respectively. Compared with non-mycorrhizal E. grandis seedlings, the root length, root diameter, root surface area, root volume, root-shoot ratio, and nitrogen, phosphorus and potassium content in roots and leaves of mycorrhizal E. grandis seedlings were significantly increased (P<0.05). 2) With the increase ofR. solanacearum infection time, activities of defense-related enzymes in mycorrhizal Eucalyptus leaves were significantly higher than those in the corresponding non-mycorrhizal Eucalyptus leaves. Peroxidase (POD), polyphenol oxidase (PPO) and β-1,3-glucanase activity increased firstly and then decreased in tissues of mycorrhizal Eucalyptus, reaching the peak at 48, 24 and 144 hpi, respectively. In leaves of mycorrhizal Eucalyptus, activities of superoxide dismutase (SOD), phenylalanine ammonia-lyase (PAL) and chitinase were first increased then declined, reaching the peak after 96 hpi of R. solanacearum infection. 3) The control effect of inoculating F. mosseae on bacterial wilt of Eucalyptus was 81.67%. Conclusion: Inoculation with F. mosseae significantly promotes the growth of Eucalyptus seedlings. After infection with R. solanacearum, activities of defense-related enzymes rapidly and greatly increase in the mycorrhizal Eucalyptus seedlings, enhancing the host defense ability against R. solanacearum.

Key words: Eucalyptus grandis, Ralstonia solanacearum, arbuscular mycorrhizal fungi, disease resistance, physiological and biochemical indicators

CLC Number:

S723.1

Di Huang,Yuan Chen,Luolong Zhong,Jiajun Liang,Zhengmu Wang,Zujing Chen. Growth and Defense-related Enzymes of Eucalyptus in Responses to Funneliformis mosseae and Ralstonia solanacearum[J]. Scientia Silvae Sinicae, 2023, 59(11): 68-75.

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处理Treatments	非菌根化 Non-mycorrhizal	菌根化Mycorrhizal
根长Root length/cm	13.18±1.12	23.55±1.83*
根直径Root diameter/mm	13.89±0.00	19.24±0.04*
根表面积Root surface area/cm²	275.57±15.58	453.00±35.35*
根体积Root volume/cm³	0.31±0.05	1.10±0.09*
根冠比Root shoot ratio	1.06±0.06	2.29±0.08*
地上氮含量 Aboveground nitrogen content/(g·kg^?1)	10.49±0.04	28.61±0.24*
地上磷含量 Aboveground phosphorus content /(g·kg^?1)	0.55±0.03	1.62±0.01*
地上钾含量 Aboveground potassium content/(g·kg^?1)	26.71±0.21	45.87±0.12*
地下氮含量 Underground nitrogen content /（g·kg^?1）	4.12±0.03	7.09±0.11*
地下磷含量 Underground phosphorus content / （g·kg^?1）	0.43±0.02	0.82±0.01*
地下钾含量 Underground potassium content /(g·kg^?1）	6.92±0.12	9.92±0.16*

处理Treatments	发病率 Incidence rate (%)	病情指数 Disease index (%)	防控效果 Control effect (%)
非菌根化 Non-mycorrhizal	100%	100%	—
菌根化 Mycorrhizal	18.33%±1.45%	15%±1.15%	81.67%±1.45%

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Growth and Defense-related Enzymes of Eucalyptus in Responses to Funneliformis mosseae and Ralstonia solanacearum

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