植物促生微生物对枫香叶色素组成和根际微生物群落的影响

doi:10.11707/j.1001-7488.LYKX20230447

Abstract

Abstract:

Objective: In this study, plant growth-promoting microorganisms (PGPM) were used to inoculate into the rhizosphere of Liquidambar formosana to investigate effects of PGPM on soil physicochemical properties and the leaf color of L. formosana, as well as changes in the composition and diversity characteristics of the rhizosphere microbial community, in order to study the effect of PGPM on plants and soil microorganisms. Method: A field inoculation experiment was conducted on 7-year-old L. formosana seedlings with consistent growth in Jurong Forest Farm of Jurong City with phosphate solubilizing bacteria Serratia marcescens subsp. marcescens (NJ2D) and Funneliformis mosseae (BJ04), and the effects on soil physical and chemical properties, leaf color parameters in autumn and soil microbial community were analyzed. Result: 1) The total nitrogen, total phosphorus, soil organic matter, available phosphorus content, soil moisture content (%), and soil pH of the rhizosphere soil of L. formosana in the treatment group were significantly increased compared to the control group (P<0.05). 2) The content of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in leaves of L. formosana inoculated once with BJ04 significantly increased (P<0.05), while NJ2D+BJ04 inoculation significantly increased the anthocyanin level in the leaves (P<0.05). 3) There were significant differences in the rhizosphere soil microbial community diversity between control group (CK), NJ2D, BJ04 and NJ2D+BJ04 treatments (P<0.05). The number of OTUs (operating taxonomic units) of rhizosphere bacteria in L. formosana trees inoculated with NJ2D, BJ04, and NJ2D+BJ04 was significantly increased compared to the control group (P<0.05), while the number of OTUs of arbuscular mycorrhizal fungi (AMF) was significantly reduced compared to the control group (P<0.05). 4) The high-throughput sequencing results of rhizosphere microorganisms showed that Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria and Gemmatimonadetes dominated the bacterial communities on the rhizosphere of L. formosana. Within the AMF community, Glomus, Rhizophagous and Dominika were dominant genera. However, after inoculation, the species abundances of Acidobacteria, Chloroflexi and Planctomycetes were significantly reduced within the rhizosphere of L. formosana (P<0.05), whereas Proteobacteria and Actinobacteria were significantly increased (P<0.05). Conclusion: Inoculation with phosphorus solubilizing bacteria, arbuscular mycorrhizal fungi, and composite microbial agents NJ2D+BJ04 can all influence the soil microenvironment and have an impact on the autumn colour of L. formosana leaves.

Key words: rhizosphere microbiome, soil physicochemical properties, leaf pigment, biological interaction, Liquidambar formosana

CLC Number:

S725
Q938

Zhuhua Wu,Juan Song,Shulin Zhu,Xing Zhao,Xuexiang Yang,Jiahong Ren,Fengmao Chen. Effects of Plant Growth-Promoting Microorganisms on Rhizosphere Microbial Community and the Leaf Pigment Composition of Liquidambar formosana[J]. Scientia Silvae Sinicae, 2023, 59(12): 125-136.

Figures/Tables 8

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References 0

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处理Treatment	全氮含量 Total nitrogen content /(g·kg^?1)	全磷含量 Total phosphorus content /(g·kg^?1)	全钾含量 Total potassium content /(g·kg^?1)	有机质含量 Organic matter content /(g·kg^?1)	有效磷含量 Available phosphorus content /(mg·kg^?1)	含水率 Water content （%）	pH
CK	0.82±0.03b	0.114±0.00 b	14.25±0.09a	7.14 ±0.18c	0.22±0.02d	2.5±0.2b	6.88±0.12d
NJ2D	0.94±0.05ab	0.116 ±0.002ab	13.99±0.65a	9.78 ±0.26b	0.78 ±0.02b	2.8±0.1ab	8.40±0.08a
BJ04	0.88±0.06b	0.120 ±0.001a	14.93±0.42a	13.21±0.99a	1.05 ±0.03a	3.0±0.1a	7.22 ±0.03c
NJ2D+BJ04	1.09±0.07a	0.119 ±0.001ab	13.96±0.62a	13.88 ±0.67a	0.31 ±0.03c	3.1±0.5a	7.88±0.05b

项目Item	多样性指数Diversity	CK	NJ2D	BJ04	NJ2D+BJ04
细菌 Bacteria	chao1	3 345.01 ±105.89b	4 335.96 ±158.30a	4 239.23 ±78.57 a	4 379.43 ±266.44 a
	Shannon	9.24 ±0.05b	9.69 ±0.09a	9.54 ±0.10 a	9.68 ±0.11 a
	goods_coverage	0.987 ±0.000a	0.981 ±0.001b	0.982 ±0.001 b	0.986 ±0.001a
AMF	chao1	286.93 ±1.08a	227.25 ±10.42c	251.07 ±5.06 b	232.32 ±8.20 bc
	Shannon	3.62 ±0.03b	3.40 ±0.06c	4.03 ±0.04 a	3.89 ±0.02 a
	goods_coverage	0.999 ±0.000a	0.999 ±0.000a	0.999 ±0.000 a	0.999 ±0.000 a

指标Item	pH	含水率 Water content	有效磷含量 Available phosphorus	有机质含量 Organic matter	全钾含量 Total potassium
土壤全磷含量 Total phosphorus content	?0.323	?0.072	0.214	0.495	0.235
土壤全氮含量 Total nitiogen content	?0.037	0.296	?0.351	0.632**	0.497
类胡萝卜素含量 Carotenoids content	?0.683**	0.174	0.662**	0.160	0.168
花青素含量 Anthocyanin content	0.087	0.128	?0.491	0.477	?0.203
叶绿素a含量 Chlorophyll a content	?0.654*	0.207	0.649**	0.242	0.136
叶绿素b含量 Chlorophyll b content	?0.637**	0.211	0.654*	0.252	0.128
总叶绿素含量 Total chlorophyll content	?0.650**	0.208	0.650*	0.245	0.134
chao1(Bacteria)	0.538	0.014	0.073	0.042	0.030
chao1(AMF)	?0.789**	?0.125	0.175	?0.298	0.011

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Effects of Plant Growth-Promoting Microorganisms on Rhizosphere Microbial Community and the Leaf Pigment Composition of Liquidambar formosana

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