Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (5): 93-101.doi: 10.11707/j.1001-7488.20220510
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Lina Han,Xianan Xie,Hui Chen,Ming Tang*
Received:
2021-09-09
Online:
2022-05-25
Published:
2022-08-19
Contact:
Ming Tang
CLC Number:
Lina Han,Xianan Xie,Hui Chen,Ming Tang. Molecular Characteristics and Function of the Metal Tolerant Protein, EgMTP6 in Eucalyptus grandis[J]. Scientia Silvae Sinicae, 2022, 58(5): 93-101.
Table 1
Sequences of the primers used in this work"
引物名称 Primer names | 引物序列(5′-3’) Primer sequences (5′-3′) | 用途 Usage |
EgMTP6-F | ATGGGCTTCAGATTTTGGAAC | 片段扩增 |
EgMTP6-R | TCATTTCTGATTCATT TCTTCTAA | Fragment amplified |
EgMTP6-TF | CGGGTTCGAAATCGATGGATCCATGGGCTTCAGATTTTGGAAC | 烟草亚细胞定位 |
EgMTP6-TR | CGCGTCCTAGGCTACGTAGGATCCTCATTTCTGATTCATTTCTTCTAA | Subcellular localization in tobacco |
EgMTP6-YF | TTGCGGCCGCATGGGCTTCAGATTTTGGAAC | 酵母异源表达 |
EgMTP6-YR | TTGCGGCCGCTCATTTCTGATTCATTTCTTCTAA | Heterologous expression in yeast |
EgMTP6-qF | TGGTTGCTCTTCTTGGTGTAG | 实时荧光定量PCR |
EgMTP6-qR | CCAGTTTCAAGTCCAGCCTTA | Quantitative Real-time PCR |
EgUBI3-qF | TCACCTACGTCTACCAGAAGG | 实时荧光定量PCR |
EgUBI3-qR | TCCTCGAAAGCTGTAAACATGG | Quantitative Real-time PCR |
Fig.1
Structure features of EgMTP6 A. ATG: Start codon; TGA: Stop codon; Black box: Exons; Gray lines: Introns; B. PCR-amplified product were gel electrophoresed; C. HD motifs were indicated by arrows; TM1-TM5: Transmembrane domain; N: -NH2; C: -COOH; D. The 3D model of EgMTP6 was constructed with 3h90.1.A as template."
Fig.4
Subcellular localization of EgMTP6 A. the green fluorescence protein of 35S∷eGFP。B. fluorescence of co-transfering with endoplasmic reticulum marker (HEDL∷mCherry) of EgMTP6; GFP: Green fluorescence protein channe; BF: Bright field; GFP+BF: Green fluorescence protein channel overlapped with bright field; mCherry: Red fluorescence protein channel; GFP+mCherry: Green fluorescence protein channel overlapped with red fluorescence protein channel. Bar: 50 μm."
Fig.7
Effect of AMF on EgMTP6 expression A. colonization morphology of AMF, a: Arbuscules, ih: Intraradical hyphae; B. AMF colonization, F%: The frequency of colonization, A%: The abundance of arbuscule abundance; C. Effect of R. irregularis on EgMTP6 expression, AM: AMF colonized roots, NM: AMF uncolonized roots."
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