Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (8): 126-135.doi: 10.11707/j.1001-7488.20220813
• Research papers • Previous Articles Next Articles
Yang Wang,Fuhao Zhang,Min Dou,Ting Xu,Hao Chen*
Received:
2021-10-13
Online:
2022-08-25
Published:
2022-12-19
Contact:
Hao Chen
CLC Number:
Yang Wang,Fuhao Zhang,Min Dou,Ting Xu,Hao Chen. Optimization of Plant Regeneration System of Litsea cubeba[J]. Scientia Silvae Sinicae, 2022, 58(8): 126-135.
Table 1
Effects of plant growth regulators on callus induction of L. cubeba stems"
处理编号 Treatment No. | 植物生长调节剂质量浓度 Mass concentration of plant growth regulator/(mg·L-1) | 诱导率 Induction rate (%) | 平均褐化率 Average browning rate (%) | 愈伤组织生长状况 The growth of the callus | ||||
6-BA | NAA | 2, 4-D | 质地 Texture | 诱导量 Induced amount | 状态 State | |||
CK | 0.0 | 0.0 | 0.0 | 0 | 0.00±0.00f | — | — | — |
1 | 0.2 | 0.0 | 0.5 | 100 | 0.00±0.00f | 较致密 More compact | 少 Few | 一般 General |
2 | 0.2 | 0.3 | 0.0 | 100 | 0.00±0.00f | 致密 Compact | 多 Many | 好 Good |
3 | 0.2 | 0.5 | 1.0 | 100 | 7.78±6.94ef | 致密 Compact | 少 Few | 好 Good |
4 | 1.0 | 0.0 | 1.0 | 100 | 27.78±11.70c | 较致密 More compact | 极少 Minute | 一般 General |
5 | 1.0 | 0.3 | 0.5 | 100 | 23.33±6.67c | 致密 Compact | 少 Few | 一般 General |
6 | 1.0 | 0.5 | 0.0 | 100 | 21.11±5.09c | 致密 Compact | 少 Few | 一般 General |
7 | 2.0 | 0.0 | 0.0 | 100 | 66.67±11.55a | 疏松 Friable | 极少 Minute | 差 Poor |
8 | 2.0 | 0.3 | 1.0 | 100 | 61.11±1.92ab | 疏松 Friable | 极少 Minute | 差 Poor |
9 | 2.0 | 0.5 | 0.5 | 100 | 52.22±8.39b | 疏松 Friable | 极少 Minute | 差 Poor |
K1 | 2.59 | 31.48 | 29.26 | |||||
K2 | 24.08 | 28.15 | 25.18 | |||||
K3 | 60.00 | 27.04 | 32.22 | |||||
R | 57.41 | 4.44 | 7.04 |
Fig.1
Process of callus induction and plant regeneration from stem segments of L. cubeba A: Callus in 7 days of induction culture; B: Callus in 28 days of induction culture; C: Callus in 7 days of proliferation culture; D: Callus in 28 days of proliferation culture; E: Adventitious bud in 7 days of differentiation culture; F: Adventitious bud in 14 days of differentiation culture; G: Adventitious bud in 21 days of differentiation culture; H: Adventitious bud in 28 days of differentiation culture; I: Plantlet in 28 days of rooting culture; J: Regenerated plants; K: Regenerated plants in 7 days after transplanting; L: Regenerated plants in 28 days after transplanting."
Table 2
Effects of plant growth regulators on the subculture of callus of L. cubeba"
处理编号 Treatment No. | 植物生长调节剂质量浓度 Mass concentration of plant growth regulator/(mg·L-1) | 增殖系数 Proliferation coefficient | 愈伤组织质地及生长状态 Callus texture and growth status | |
6-BA | NAA | |||
1 | 0.5 | 0.2 | 2.33±0.48bc | 疏松颗粒状,生长状态一般 Loose granular,general growth |
2 | 1.0 | 0.2 | 1.81±0.32cd | 疏松颗粒状,生长状态一般 Loose granular,general growth |
3 | 1.5 | 0.2 | 1.10±0.05d | 疏松水渍状,生长状态差 Loose water stains,poor growth |
4 | 0.5 | 0.5 | 2.55±0.26b | 致密型,生长状态良好 Compact,good growth |
5 | 1.0 | 0.5 | 2.14±0.39bc | 疏松颗粒状,生长状态良好 Loose granular,good growth |
6 | 1.5 | 0.5 | 1.25±0.10d | 疏松水渍状,生长状态差 Loose water stains,poor growth |
7 | 0.5 | 1.0 | 3.43±0.89a | 致密型,生长状态良好 Compact,good growth |
8 | 1.0 | 1.0 | 3.80±0.22a | 疏松颗粒状,生长状态良好 Loose granular,good growth |
9 | 1.5 | 1.0 | 1.30±0.09d | 疏松水渍状,生长状态差 Loose water stains,poor growth |
K1 | 2.77 | 1.75 | ||
K2 | 2.58 | 1.98 | ||
K3 | 1.21 | 2.84 | ||
R | 1.56 | 1.09 |
Table 3
Effect of plant growth regulators on adventitious bud differentiation of L. cubeba"
处理编号 Treatment No. | 植物生长调节剂质量浓度 Mass concentration of plant growth regulator/(mg·L-1) | 不定芽分化率 Adventitious bud differentiation rate (%) | 平均不定芽数 Average adventitious bud number | 不定芽分化及生长状态 Adventitious bud differentiation and growth status | |
6-BA | IBA | ||||
CK | 0 | 0 | 0.00±0.00f | 0.00±0.00f | 未分化 Undifferentiated |
1 | 0.5 | 0.1 | 45.59±3.82a | 8.14±0.82a | 分化旺盛,长势好 Exuberant differentiation,good growth |
2 | 1.0 | 0.1 | 25.56±5.09bc | 5.75±0.25bc | 分化旺盛,长势一般 Exuberant differentiation,general growth |
3 | 2.0 | 0.1 | 18.89±1.92d | 5.38±0.21bc | 分化一般,长势一般 General differentiation,general growth |
4 | 0.5 | 0.5 | 31.11±5.09b | 6.06±0.35b | 分化旺盛,长势好 Exuberant differentiation,good growth |
5 | 1.0 | 0.5 | 23.33±3.34cd | 4.82±0.79cd | 分化一般,长势一般 Normal differentiation,general growth |
6 | 2.0 | 0.5 | 17.78±3.85d | 4.08±0.22d | 分化一般,长势一般 Normal differentiation,general growth |
7 | 0.5 | 1.0 | 0.00±0.00f | 0.00±0.00f | 未分化 Undifferentiated |
8 | 1.0 | 1.0 | 7.78±1.92e | 2.67±1.04e | 分化差,长势一般 Poor differentiation,general growth |
9 | 2.0 | 1.0 | 5.55±3.85ef | 1.78±0.69e | 分化差,长势差 Poor differentiation,poor growth |
K1 | 25.57 | 30.01 | |||
K2 | 18.89 | 24.07 | |||
K3 | 14.07 | 4.44 | |||
R | 11.50 | 25.57 | |||
k1 | 4.73 | 6.42 | |||
k2 | 4.41 | 4.99 | |||
k3 | 3.75 | 1.48 | |||
r | 0.98 | 4.94 |
Table 4
Effects of IBA and activated carbon on rooting of adventitious buds of L. cubeba"
处理编号 Treatment No. | IBA/(mg·L-1) | 活性炭/(g·L-1) | 平均生根率 Average rooting rate (%) | 平均根数 The average number of roots | 根系生长状况 Root growth status |
CK | 0 | 0 | 23.33±11.55f | 2.11±0.77c | 根数少、细长 Few and slender roots |
1 | 0.2 | 0 | 66.67±5.77b | 4.94±0.31b | 根数多、粗壮 Many and stout roots |
2 | 0.5 | 0 | 93.33±5.77a | 6.74±0.79a | 根数多、粗壮 Many and stout roots |
3 | 1.0 | 0 | 73.33±11.55b | 6.13±0.76a | 根数多、粗壮 Many and stout roots |
4 | 0.2 | 0.5 | 56.67±15.28bcd | 2.33±0.50c | 根数少、细长 Few and slender roots |
5 | 0.5 | 0.5 | 63.33±15.28bc | 2.39±0.22c | 根数少、细长 Few and slender roots |
6 | 1.0 | 0.5 | 60.00±20.00bc | 2.89±0.72c | 根数少、细长 Few and slender roots |
7 | 0.2 | 1.0 | 40.00±10.00de | 2.20±0.37c | 根数少、细长 Few and slender roots |
8 | 0.5 | 1.0 | 53.33±5.77bcd | 2.19±0.02c | 根数少、细长 Few and slender roots |
9 | 1.0 | 1.0 | 43.33±5.77cde | 2.17±0.14c | 根数少、细长 Few and slender roots |
K1 | 54.45 | 77.78 | |||
K2 | 70.00 | 60.00 | |||
K3 | 58.89 | 45.55 | |||
R | 15.55 | 32.23 | |||
k1 | 3.16 | 5.94 | |||
k2 | 3.77 | 2.54 | |||
k3 | 3.73 | 2.19 | |||
r | 0.61 | 3.75 |
Table 5
Effects of substrate on survival of transplanting regenerated plants of L. cubeba"
处理编号 Treatment No. | 移栽基质 Transplanting substrate | 移栽数量 Transplanting number | 成活数量 Survival number | 成活率 Survival rate (%) |
1 | 营养土 Nutrient soil | 18 | 13 | 72.22 |
2 | 营养土∶珍珠岩= 3∶1(V/V) Nutrient soil∶ perlite = 3∶1(V/V) | 18 | 15 | 83.33 |
3 | 红壤土∶营养土∶珍珠岩= 2∶1/1(V/V) Red soil∶ nutrient soil∶ perlite = 2∶1∶1(V/V) | 18 | 5 | 27.78 |
4 | 红壤土∶营养土∶珍珠岩= 1∶1∶1(V/V) Red soil∶ nutrient soil∶ perlite = 1∶1∶1(V/V) | 18 | 7 | 38.89 |
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