Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (2): 148-158.doi: 10.11707/j.1001-7488.20220215
• Research papers • Previous Articles Next Articles
Shunfu Li,Huimin Wang,Lisha Fang,Zhen Liu*
Received:
2021-02-03
Online:
2022-02-25
Published:
2022-04-26
Contact:
Zhen Liu
CLC Number:
Shunfu Li,Huimin Wang,Lisha Fang,Zhen Liu. Gene Expression Related to Terminal Bud Death Induced by Short-Day in Paulownia fortunei[J]. Scientia Silvae Sinicae, 2022, 58(2): 148-158.
Fig.1
The changes of terminal bud and average height of Paulownia fortunei induced by short day A: 25 ℃, 8 h light/16 h dark for 14 days(SDa: Height growth period), 28 days(SDb: Height growth cessation period), 42 days(SDc: Terminal bud death period); B: Measure the height of P. fortunei every 7 days."
Table 1
The reads quality statistics of terminal buds of Paulownia fortunei induced by short day at three stages"
样品 Sample | 原始数据 Raw reads | 有效数据 Clean reads | 有效碱基数 Clean bases/Gb | Q30(%) | 有效读数比例 Clean reads ratio (%) |
SDa-1 | 47 170 900 | 46 155 682 | 6.45 | 93.51 | 97.85 |
SDa-2 | 47 296 568 | 46 028 158 | 6.43 | 93.84 | 97.32 |
SDa-3 | 47 694 770 | 46 368 244 | 6.48 | 93.96 | 97.22 |
SDb-1 | 48 454 868 | 47 249 798 | 6.60 | 92.85 | 97.51 |
SDb-2 | 45 454 586 | 44 452 908 | 6.21 | 93.76 | 97.80 |
SDb-3 | 45 592 110 | 44 391 996 | 6.20 | 93.77 | 97.37 |
SDc-1 | 49 038 356 | 47 567 810 | 6.64 | 94.58 | 97.00 |
SDc-2 | 48 460 682 | 46 921 468 | 6.55 | 93.50 | 96.82 |
SDc-3 | 44 069 318 | 42 734 802 | 6.01 | 93.71 | 96.97 |
Table 2
The functional annotation statistical results of the differentially expressed genes"
被注释基因 Annotated genes | 总数 Total | 数据库 Databases | 总体 Overall | ||||||
NR | NT | GO | EggNOG | KEGG | UniProt | Pfam | |||
数目 Number | 44 937 | 33 810 | 20 559 | 22 111 | 13 328 | 12 540 | 25 396 | 19 101 | 37 076 |
百分比 Percentage(%) | 100 | 75.24 | 45.75 | 49.20 | 29.66 | 27.91 | 56.51 | 42.51 | 83.51 |
Fig.3
GO clustering analysis of differentially expressed genes at different stages 1-17: Cellular component; 18-43: Biological process; 44-57: Molecular function. 1. Extracellular region; 2. Cell; 3. Nucleoid; 4. Membrane; 5. Cell junction; 6. Membrane-enclosed lumen; 7. Macromolecular complex; 8. Organelle; 9. Other organism part; 10. Extracellular region part; 11. Organelle part; 12. Virion part; 13. Membrane part; 14. Synapse part; 15. Cell part; 16. Synapse; 17. Supramolecular complex; 18. Reproduction; 19. Cell killing; 20. Immune system process; 21. Behavior; 22. Metabolic process; 23. Cell proliferation; 24. Cellular process; 25. Carbon utilization; 26. Nitrogen utilization; 27. Reproductive process; 28. Biological adhesion; 29. Signaling; 30. Multicellular organismal process; 31. Developmental process; 32. Growth; 33. Locomotion; 34. Pigmentation; 35. Rhythmic process; 36. Response to stimulus; 37. Localization; 38. Multi-organism process; 39. Biological regulation; 40. Cellular component organization or biogenesis; 41. Cell aggregation; 42. Detoxification; 43. Presynaptic process involved in chemical synaptic transmission; 44. Catalytic activity; 45. Signal transducer activity; 46. Structural molecule activity; 47. Transporter activity; 48. Binding activity; 49. Antioxidant activity; 50. Protein tag; 51. Translation regulator activity; 52. Nutrient reservoir activity; 53. Molecular transducer activity; 54. Molecular function regulator; 55. Hijacked molecular function; 56. Molecular carrier activity; 57. Transcription regulator activity."
Table 3
KEGG enrichment analysis of differentially expressed genes"
通路名称 Pathway | 上调基因数 UP regulated genes | 下调基因数 Down regulated genes | 校正P值 Corrected P value | 通路位置 Pathway ID |
SDb vs SDa | ||||
植物激素信号转导Plant hormone signal transduction | 36 | 59 | 1.06×10-10 | map04075 |
角质、木栓质和蜡的合成Cutin, suberine and wax biosynthesis | 19 | 7 | 2.56×10-7 | map00073 |
光合作用天线蛋白Photosynthesis-antenna proteins | 0 | 18 | 5.07×10-6 | map00196 |
苯丙烷类合成Phenylpropanoid biosynthesis | 36 | 20 | 8.72×10-6 | map00940 |
MAPK信号通路MAPK signaling pathway-plant | 46 | 21 | 9.74×10-5 | map04016 |
乙醛酸和二羧酸代谢Glyoxylate and dicarboxylate metabolism | 64 | 11 | 0.000 2 | map00630 |
光合作用Photosynthesis | 1 | 19 | 0.000 7 | map00195 |
碳代谢作用Carbon metabolism | 175 | 12 | 0.007 1 | map01200 |
SDc vs SDb | ||||
植物激素信号转导Plant hormone signal transduction | 26 | 84 | 0.000 2 | map04075 |
氨基酸合成Biosynthesis of amino acids | 197 | 76 | 0.001 5 | map01230 |
淀粉和蔗糖代谢Starch and sucrose metabolism | 95 | 58 | 0.001 9 | map00500 |
苯丙氨酸等合成Phenylalanine, tyrosine and tryptophan biosynthesis | 40 | 15 | 0.004 6 | map00400 |
SDc vs SDa | ||||
植物激素信号转导Plant hormone signal transduction | 59 | 93 | 3.94×10-11 | map04075 |
淀粉和蔗糖代谢Starch and sucrose metabolism | 146 | 67 | 1.25×10-10 | map00500 |
苯丙烷类合成Phenylpropanoid biosynthesis | 59 | 42 | 1.94×10-9 | map00940 |
MAPK信号通路MAPK signaling pathway-plant | 72 | 44 | 9.89×10-5 | map04016 |
氰氨基酸代谢Cyanoamino acid metabolism | 48 | 21 | 1.77×10-5 | map00460 |
吲哚生物碱生物合成Indole alkaloid biosynthesis | 9 | 13 | 5.27×10-5 | map00901 |
角质、木栓质和蜡的合成Cutin, suberine and wax biosynthesis | 17 | 14 | 0.000 2 | map00073 |
苯丙氨酸等合成Phenylalanine, tyrosine and tryptophan biosynthesis | 45 | 19 | 0.001 6 | map00400 |
乙醛酸和二羧酸代谢Glyoxylate and dicarboxylate metabolism | 103 | 19 | 0.001 9 | map00630 |
氨基糖核苷酸糖代谢Amino sugar and nucleotide sugar metabolism | 103 | 56 | 0.007 3 | map00520 |
Fig.4
KEGG pathway of hormone signal transduction and heat map of the differentially expressed genes induced by short day IAA_DN64977_c0_g1 and IAA_DN70584_c2_g2 indicate that the two differentially expressed genes of IAA are enriched in the KEGG pathway in three samples, and the gene number was assembled by Trinity software."
Table 4
Function of differentially expressed genes in KEGG pathway of plant hormone signal transduction"
植物激素 Hormone | 基因编号 Gene number | KO ID | 基因功能描述 Function description |
生长素Auxin | DN80863_c0_g1 DN81206_c0_g2 DN64977_c0_g1 DN70584_c2_g2 DN77909_c1_g1 DN79226_c1_g1 DN67543_c0_g1 DN63863_c0_g1 | K13946 K14485 K14484 K14486 K14487 K14488 | 生长输入载体AUX1 Auxin influx carrier AUX1(AUX1) 转运响应抑制因子1 Transport inhibitor response 1 (TIR1) 生长素响应蛋白IAA Auxin responsive protein (IAA) 生长素响应因子 Auxin response factor (ARF) 生长素响应GH3基因家族 Auxin responsive GH3 gene family (GH3) SAUR家族蛋白SAUR family protein(SAUR) |
细胞分裂素Cytokinin | DN68870_c1_g5 | K14491 | 双组分响应调节器ARR-B家族 Two component response regulator ARR-B family (ARR-B) |
赤霉素Gibberellin | DN82220_c1_g1 DN82158_c1_g2 DN81860_c1_g1 DN81860_c1_g2 | K14493 K14494 | 赤霉素受体GID1 Gibberellin receptor GID1 (GID1) DELLA蛋白 DELLA protein (DELLA) |
脱落酸Abscisic acid | DN67794_c1_g1 DN59959_c0_g1 DN81200_c0_g1 DN79605_c0_g2 DN79605_c0_g3 DN83661_c2_g2 | K14496 K14497 K14498 K14432 | 脱落酸受体PYR/PYL蛋白家族Abscisic acid receptor PYR/PYL family (PYL) 蛋白磷酸酶2C Protein phosphatase 2C (PP2C) 蔗糖非酵解型蛋白激酶2 Sucrose non-fermenting1-related protein kinase 2 (SNRK2) ABA反应元件结合因子 ABA responsive element binding factor (ABF) |
乙烯Ethylene | DN75732_c0_g1 DN75455_c0_g1 DN82109_c0_g1 DN72495_c1_g1 DN72737_c2_g4 | K14510 K14513 K14514 K14516 | 丝氨酸/苏氨酸蛋白激酶CTR1 Serine/threonine-protein kinase CTR1 (CTR1) 乙烯不敏感蛋白2 Ethylene insensitive protein 2 (EIN2) 乙烯不敏感蛋白3 Ethylene insensitive protein 3 (EIN3) 乙烯应答转录因子1 Ethylene responsive transcription factor 1 (ERF1) |
油菜素内酯Brassinolide | DN80256_c0_g5 | K13416 | 油菜素内酯不敏感相关受体激酶1 Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) |
DN74700_c1_g1 | K14499 | BRI1激酶抑制子1 BRI1 kinase inhibitor 1 (BKI1) | |
DN80006_c0_g7 | K14500 | BR信号激酶BR signaling kinase (BSK) | |
DN78469_c0_g1 | K14503 | 油菜素内酯抗性因子1/2 Brassinosteroid resistant 1/2 (BZR1/2) | |
DN80006_c0_g7 | K14504 | 木糖葡萄糖基转移酶TCH4 Xyloglucosyl transferase TCH4 (TCH4) | |
DN78469_c0_g1 | K14505 | 细胞周期蛋白D3 Cyclin D3 (CYCD3) |
Table 5
The gene ID and primers of differentially expressed genes by qRT-PCR"
基因编号 Gene number | 基因功能描述 Function description | 引物序列 Primer sequence (5’- 3’) | KO ID |
DN82158_c1_g1 | 赤霉素受体GID1 Gibberellin receptor GID1 (GID1) | F: TGCGGGGTGAAAACAATAG R: TCAGAGTAACACGGGAAGAAAT | K14493 |
DN81200_c0_g1 | 蔗糖非酵解型蛋白激酶2 Sucrose non-fermenting1-related protein kinase 2 (SNRK2) | F: TGTTTTGCCTCTTTGATTGTTC R: CTCACCTTCTTGCCCCTCT | K14498 |
DN81546_c5_g3 | 光系统Ⅰ亚单位F Photosystem I subunit F (PSI-F) | F: AGTGAGGCAAACCATCAGAAC R: CGAGAAGCAGGAGATCAAGAA | K02694 |
DN67975_c4_g7 | 茉莉酸氨基合成酶JAR1 Jasmonic acid resistant 1 (JAR1) | F: GCTTGGCTGCTGCTTCTACA R: TCAAGGGTTCCACAACTCCAC | K14506 |
DN68587_c3_g1 | 采光复合物II叶绿素a/b结合蛋白2 Light harvesting complex II chlorophyll a/b binding protein 2 (LHCB2) | F: GCCTCCTCCAACTCTGTATCC R: CGTCTTCCCCGAAATCCT | K08913 |
DN74090_c1_g2 | 采光复合物II叶绿素a/b结合蛋白4 Light harvesting complex II chlorophyll a/b binding protein 4 (LHCB4) | F: CTGGATGGCTCCCTCGTT R: GCTGTAAGGCTGGAATGGC | K08915 |
DN68271_c1_g1 | 采光复合物II叶绿素a/b结合蛋白6 Light harvesting complex II chlorophyll a/b binding protein 6 (LHCB6) | F: GTTGCTTTTCCTACTCGGTTTA R: GCTTCTGGACTCTGGCACTAT | K08917 |
DN80256_c0_g5 | 油菜素内酯不敏感相关受体激酶1 Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) | F: CGCTGCCCTTCTATTTGCT R: GAACTTCGGGGTCCTCCTC | K13416 |
DN67597_c0_g3 | 光系统Ⅰ亚单位D Photosystem I subunit D (PSI-D) | F: GAGAATTGTGCAAGGGACTGT R: CCACCCCTCCGAACTCAT | K02692 |
DN68054_c0_g4 | TGA转录因子TGA transcription factor (TGA) | F: TGCTTGCTTTCGGGGAGT R: GCTGCTTGTGCCATTTTGTAG | K14431 |
DN81860_c1_g2 | DELLA蛋白DELLA protein (DELLA) | F: TTGCATATCTGCCTCCCTAAAT R: AATGAACCCGAAAATCGTGAC | K14494 |
18S rRNA | 18S核糖体RNA 18S ribosomal RNA | F: ACATAGTAAGGATTGACAGA R: TAACGGAATTAACCAGACA |
曹喜兵. 2014. 甲基磺酸甲酯对丛枝病泡桐基因表达影响研究. 郑州: 河南农业大学. | |
Cao X B. 2014. Research of the gene expression of Paulownia witches' broom under methyl methane sulfonate treatment. Zhengzhou: Henan Agricultural University. [in Chinese] | |
侯元凯, 翟明普, 聂爱社, 等. 兰考泡桐苗木顶芽水分变化规律研究. 北京林业大学学报, 2001, 23 (6): 17- 21. | |
Hou Y K , Zhai M P , Nie A S , et al. Law of water change of top buds of Paulownia elongata seedlings. Journal of Beijing Forestry University, 2001, 23 (6): 17- 21. | |
简令成, 卢存福, 邓江明, 等. 木本植物休眠的诱导因子及其细胞内Ca2+水平的调节作用. 应用与环境生物学报, 2004, 10 (1): 1- 6. | |
Jian L C , Lu C F , Deng J M , et al. Inducing factor and regulating role of intracellular Ca2+ level for woody plant bud dormancy. Chinese Journal of Applied and Environmental Biology, 2004, 10 (1): 1- 6. | |
蒋建平. 泡桐栽培学. 北京: 中国林业出版社, 1990: 263- 283. | |
Jiang J P . Paulownia cultivation. Beijing: China Forestry Publishing House, 1990: 263- 283. | |
刘杭, 杨贺忠, 李亮, 等. 梨休眠DELLA蛋白PpGAI基因的克隆与表达分析. 分子植物育种, 2016, 14 (8): 1995- 2002. | |
Liu H , Yang H Z , Li L , et al. Cloning and expression analysis of PpGAI gene of DELLA protein related to dormancy from Pyrus. Molecular Plant Breeding, 2016, 14 (8): 1995- 2002. | |
劉震. 亜熱帯域に分布するイイギリの休眠に関する研究. 三重大学生物資源学部演習林報告, 2000, 24, 107- 161. | |
Liu Z . Studies on the dormancy in Idesia polycarpa distributing in the subtropical zone. Bulletin of the Mie University Forests, 2000, 24, 107- 161. | |
刘震, 何松林, 王艳梅, 等. 泡桐顶侧芽休眠发育的温度特性研究. 林业科学, 2004, 40 (3): 46- 50. | |
Liu Z , He S L , Wang Y M , et al. Study on the temperature characteristics of dormancy development of terminal and lateral buds in Paulownia. Scientia Silvae Sinicae, 2004, 40 (3): 46- 50. | |
刘震, 王玲. 不同种源山桐子冬芽休眠的温度特性. 河南农业大学学报, 2000, 34 (3): 252- 254. 252-254, 297 | |
Liu Z , Wang L . Temperature characteristics of winter buds dormancy in Idesia polycarpa of different origins. Journal of Henan Agricultural University, 2000, 34 (3): 252- 254. 252-254, 297 | |
王国霞, 耿晓东, 张娜, 等. 秋冬季节泡桐顶芽形态及显微结构变化分析. 西北林学院学报, 2017, 32 (1): 165- 171. | |
Wang G X , Geng X D , Zhang N , et al. Morphology and microstructure changes of terminal buds of Paulownia in autumn and winter. Journal of Northwest Forestry University, 2017, 32 (1): 165- 171. | |
王艳梅, 牛晓峰, 刘震, 等. 泡桐生长停止和顶芽死亡过程中的光合特性研究. 北京林业大学学报, 2009, 31 (6): 121- 127. | |
Wang Y M , Niu X F , Liu Z , et al. Photosynthetic characteristics of Paulownia during growth cessation and terminal buds'death. Journal of Beijing Forestry University, 2009, 31 (6): 121- 127. | |
王艳梅, 刘震, 牛晓峰. 一年生泡桐不同部位顶芽内源激素的动态变化. 林业科学, 2012, 48 (7): 61- 65. | |
Wang Y M , Liu Z , Niu X F . Dynamic change of endogenous hormones in terminal buds from different crown position of one-year-old Paulownia. Scientia Silvae Sinicae, 2012, 48 (7): 61- 65. | |
王艳梅, 马天晓, 刘震. 泡桐顶芽死亡机制研究进展. 林业科学, 2013, 49 (4): 117- 122. | |
Wang Y M , Ma T X , Liu Z . Progress in the death mechanism research of Paulownia spp. terminal buds. Scientia Silvae Sinicae, 2013, 49 (4): 117- 122. | |
王艳梅, 张龙冲, 马天晓, 等. 人工调控对泡桐顶芽生长发育的影响. 林业科学, 2016, 52 (8): 53- 59. | |
Wang Y M , Zhang L C , Ma T X , et al. Effect of artificial regulation on terminal bud growth of Paulownia spp. Scientia Silvae Sinicae, 2016, 52 (8): 53- 59. | |
叶金山, 杨文萍. 泡桐假二叉分枝机理. 东北林业大学学报, 2009, 32 (2): 6- 7. | |
Ye J S , Yang W P . Mechanism of false dichotomous branching for Paulownia. Journal of Northeast Forestry University, 2009, 37 (2): 6- 7. | |
永田洋, 中岛敦司, 万木丰. 树木の芽の休眠. 三重大学演习林报告, 1994, 18, 17- 42. | |
Nagata H , Nakashima A , Yurugi Y . Bud dormancy in woody plants. Report of Experiment Forest of Mie-University, 1994, 18, 17- 42. | |
竺肇华. 泡桐属植物的分布中心及区系成分的探讨. 林业科学, 1981, 17 (3): 271- 280. | |
Zhu Z H . A discussion on the distribution centre and flora of Paulownia genus. Scientia Silvae Sinicae, 1981, 17 (3): 271- 280. | |
Cooke J E K , Eriksson M E , Junttila O , et al. The dynamic nature of bud dormancy in trees: environmental control and molecular mechanisms. Plant, Cell and Environment, 2012, 35 (10): 1707- 1728.
doi: 10.1111/j.1365-3040.2012.02552.x |
|
den Boer B G , Murray J A . Control of plant growth and development through manipulation of cell-cycle genes. Current Opinion in Biotechnology, 2000, 11 (2): 138- 145.
doi: 10.1016/S0958-1669(00)00072-0 |
|
Gutierrez C , Ramirez P E , Castellano M M , et al. G1 to S transition: more than a cell cycle engine switch. Current Opinion in Plant Biology, 2002, 6 (5): 480- 486. | |
Horvath D P , Anderson J V , Chao W S , et al. Knowing when to grow: signals regulating bud dormancy. Trends in Plant Science, 2003, 8 (11): 534- 540.
doi: 10.1016/j.tplants.2003.09.013 |
|
Hubbarb K E , Nishimura N , Hitomi K , et al. Early abscisic acid signal transduction mechanisms: newly discovered components and newly emerging questions. Genes and Development, 2010, 24 (16): 1695- 1708.
doi: 10.1101/gad.1953910 |
|
Jian L C , Li P H , Sun L H , et al. Alterations in ultrastructure and subcellular localization of Ca2+ in poplar apical bud cells during the induction dormancy. Journal of Experimental Botany, 1997, 48 (6): 1195- 1207.
doi: 10.1093/jxb/48.6.1195 |
|
Johnson P R , Ecker J R . The ethylene gas signal transduction pathway: a molecular perspective. Annual Review of Genetics, 1998, 32 (1): 227- 254.
doi: 10.1146/annurev.genet.32.1.227 |
|
Li J Z , Xu Y , Niu Q F , et al. Abscisic acid (ABA) promotes the induction and maintenance of pear (Pyrus pyrifolia white pear group) flower bud endodormancy. International Journal of Molecular Sciences, 2018, 19 (1): 310.
doi: 10.3390/ijms19010310 |
|
Li W , Ma M , Feng Y , et al. EIN2-directed translational regulation of ethylene signaling in Arabidopsis. Cell, 2015, 163 (3): 670- 683.
doi: 10.1016/j.cell.2015.09.037 |
|
Olsen J E . Light and temperature sensing and signaling in induction of bud dormancy in woody plants. Plant Molecular Biology, 2010, 73 (s1-2): 37- 47. | |
Perry T O . Dormancy of trees in winter. Science, 1971, 3966 (171): 29- 36. | |
Rohde A , Montagu M V , Inze D , et al. Factors regulating the expression of cell cycle genes in individual buds of Populus. Planta, 1997, 201 (1): 43- 52.
doi: 10.1007/BF01258679 |
|
Rohde A , Prinsen E , Rycke R D , et al. PtABI3 impinges on the growth and differentiation of embryonic leaves during bud set in poplar. Plant Cell, 2002, 14 (8): 1885- 1901.
doi: 10.1105/tpc.003186 |
|
Ruonala R , Rinne P L , Baghour M , et al. Transitions in the functioning of the shoot apical meristem in birch (Betula pendula) involve ethylene. Plant Journal, 2006, 46 (4): 628- 640.
doi: 10.1111/j.1365-313X.2006.02722.x |
|
Ruttink T , Arend M , Morreel K , et al. A molecular timetable for apical bud formation and dormancy induction in poplar. Plant Cell, 2007, 19 (8): 2370- 2390.
doi: 10.1105/tpc.107.052811 |
|
Wang J Y , Wang H Y , Tao D , et al. Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia. Scientific Reports, 2019, 9 (1): 16602.
doi: 10.1038/s41598-019-53283-2 |
|
Wang W F , Bai M Y , Wang Z Y . The brassinosteroid signaling network-a paradigm of signal integration. Current Opinion in Plant Biology, 2014, 21, 147- 153.
doi: 10.1016/j.pbi.2014.07.012 |
|
Wang Y M , Ma T X , Liu Z . The relationship between terminal bud death and programmed cell death in Paulownia spp. Polish Journal of Environmental Studies, 2018, 27 (3): 1413- 1417.
doi: 10.15244/pjoes/76310 |
|
Xu W , Mary M P , Diana H , et al. Arabidopsis TCH4, regulated by hormones and the environment encodes a xyloglucan endotransglycosylase. Plant Cell, 1995, 7 (10): 1557- 1567. |
[1] | Zijing Zhou,Fuhua Fan,Xianwen Shang,Huijuan Qin,Conghui Wang,Guijie Ding,Jianhui Tan. Effects of Exogenous IAA on Stem Secondary Growth of Pinus massoniana Seedlings [J]. Scientia Silvae Sinicae, 2021, 57(9): 42-51. |
[2] | Xingzhou Chen,Guoying Zhou,Xinggang Chen,Lingyu Jiang,Anhua Bao,Jun Liu. Screening of Effectors of Colletotrichum fructicola in Camellia oleifera [J]. Scientia Silvae Sinicae, 2021, 57(9): 110-120. |
[3] | Xin Peng,Hantang Wang,Chunhui Guo,Zhende Yang,Jing Zhou,Xue Wang,Zhirou Ding. EST-SSR Development and Cryptic Species Identification of the Invasive Gall-Causing Pest Leptocybe invasa (Hymenopetra: Eulophidae) [J]. Scientia Silvae Sinicae, 2021, 57(9): 140-151. |
[4] | Jinfeng Cai,Xiaoming Yang,Wanwen Yu,Guibin Wang,Fuliang Cao. Development of SSR Molecular Markers Based on Transcriptome Sequencing of Melia azedarach [J]. Scientia Silvae Sinicae, 2021, 57(6): 85-92. |
[5] | Peihuang Zhu,Yu Chen,Lingzhi Zhu,Rong Li,Kongshu Ji. Codon Usage Bias and Its Influencing Factors in Pinus massoniana Transcriptome [J]. Scientia Silvae Sinicae, 2020, 56(4): 74-81. |
[6] | Xiaoyu Lu,Zhu Chen,Fei Tang,Songling Fu,Jie Ren. Combined Transcriptomic and Metabolomic Analysis Reveals Mechanism of Anthocyanin Changes in Red Maple(Acer rubrum) Leaves [J]. Scientia Silvae Sinicae, 2020, 56(1): 38-53. |
[7] | Zhao Qingquan, Chi Yujie, Zhang Jian, Feng Lianrong. Transcriptome Construction and Related Gene Expression Analysis of Lenzites gibbosa in Woody Environment [J]. Scientia Silvae Sinicae, 2019, 55(8): 95-105. |
[8] | Han Xiaohong, Lu Ciding, Hua Yin, Lin Haoyu, Shi Yufei, Wu Songqing, Zhang Feiping, Liang Guanghong. Phylogenetic Analysis of Transcriptome and Three Detoxification Enzyme Families Related Genes in Anoplophora chinensis (Coleoptera: Cerambycidae) [J]. Scientia Silvae Sinicae, 2019, 55(5): 104-113. |
[9] | Zhang Enliang, Ma Lingling, Yang Rutong, Li Linfang, Wang Qing, Li Ya, Wang Peng. Transcriptome Profiling of IBA-Induced Adventitious Root Formation in Softwood Cuttings of Catalpa bungei ‘Yu-1’ [J]. Scientia Silvae Sinicae, 2018, 54(5): 48-61. |
[10] | Mao Weibing, Chen Faju, Wang Changlan, Liang Hongwei. Transcriptome Sequencing and Analysis of Male Sterile Flower Buds in Catalpa bungei [J]. Scientia Silvae Sinicae, 2017, 53(6): 141-150. |
[11] | Shi Xiaodong, Zhu Xuehui, Sheng Yuzhen, Zhuang Guoqing, Chen Fang. Development of SSR Markers Based on Transcriptome Sequence of Phoebe zhennan [J]. Scientia Silvae Sinicae, 2016, 52(11): 71-78. |
[12] | Zhang Zhen, Zhang Hanguo, Mo Chi, Zhang Lei. Transcriptome Sequencing Analysis and Development of EST-SSR Markers for Pinus koraiensis [J]. Scientia Silvae Sinicae, 2015, 51(8): 114-120. |
[13] | Chen Hao, Tan Xiaofeng. Identification of α-Linolenic Acid Metabolism Pathway Based on Transcriptome Data of Vernicia fordii Kernels during Tung Oil Synthesis Stage [J]. Scientia Silvae Sinicae, 2015, 51(3): 41-48. |
[14] | Wen Yafeng, Han Wenjun, Zhou Hong, Xu Gangbiao. SSR Mining and Development of EST-SSR Markers for Cunninghamia lanceolata Based on Transcriptome Sequences [J]. Scientia Silvae Sinicae, 2015, 51(11): 40-49. |
[15] | Hu Yulin, Yao Xiaohua, Ren Huadong, Wang Kailiang, Lin Ping. Sequencing of Transcriptome Relevant to Flowering and Analysis of Floral-Related Genes Expression in Camellia oleifera [J]. Scientia Silvae Sinicae, 2014, 50(9): 36-43. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||