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Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (2): 148-158.doi: 10.11707/j.1001-7488.20220215

• Research papers • Previous Articles     Next Articles

Gene Expression Related to Terminal Bud Death Induced by Short-Day in Paulownia fortunei

Shunfu Li,Huimin Wang,Lisha Fang,Zhen Liu*   

  1. College of Forestry, Henan Agricultural University Zhengzhou 450002
  • Received:2021-02-03 Online:2022-02-25 Published:2022-04-26
  • Contact: Zhen Liu


Objective: The transcriptome sequencing analysis of Paulownia fortunei terminal bud induced by short days was carried out to explore the molecular mechanism of terminal bud death, so as to provide a theoretical basis for solving the problem of Paulownia "crown big stem low" Method: Under the condition of constant temperature of 25 ℃, the one-year-old seedlings of P. fortunei were induced by short days (SD) so that the terminal buds were in three periods: growth period (SDa), growth cessation period (SDb), and terminal bud death period (SDc). Terminal buds of each period were tested for transcriptome sequencing, and fluorescent quantitative PCR (qRT-PCR) was used to verify the expression of 11 genes. Result: A total of 57.47 Gb clean data were obtained from the transcriptome sequencing of P. fortunei. A total of 44 397 differentially expressed genes were screened out by comparing the samples of three periods using the software DESeq2. Among them, 37076 genes (83.51%) were annotated into seven databases (NR, NT, GO, EggNOG, KEGG, UniProt, Pfam). KEGG pathway enrichment analysis showed that the main enrichment was in the plant hormone signaling pathway. Through the analysis of the expressed genes in the plant hormone signaling pathway of SDc during the terminal bud death stage, it was concluded that the abscisic acid (ABA) signal transduction factor ABF was down-regulated, the ethylene (ETH) signal transduction factor ERF1 was up-regulated, brassinolide(BR) hormone signal transduction of TCH4 encoding xyloglucan endoglycosyltransferase and cell cycle-specific protein gene CYCD3 were up-regulated, which were different from those in most plants during bud dormancy. The result of qRT-PCR and transcriptome sequencing had deviation, but the overall trend was consistent, which proved that transcriptome sequencing result were correct and reliable. Conclusion: The expression of ABF, ERF1, TCH4 and CYCD3 in the plant hormone signal transduction pathway may lead to the continued division and differentiation of the shoot apical meristem in SDc. At the same time, the young leaves that wrapped the apical meristem fall off, so that the terminal bud of Paulownia fortunei cannot form dormant buds and result in death.

Key words: Paulownia fortunei, terminal bud death, short day, hormone signal transduction, transcriptome

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