核桃炭疽病发生相关的酚类物质代谢分析

doi:10.11707/j.1001-7488.20211007

Abstract

Abstract:

Objective: This study aimed to provide references for exploring the mechanism of walnut resistance to anthracnose. For this end, we identified 130 phenolic compounds after the infection of Colletotrichum gloeosprioides into the walnut husk, carried out absolute quantification of the compounds, analyzed their changes, and screened the potential effective components against anthracnose. Method: In this study, walnut (Juglans regia) cultivars, 'Xiangling' and 'Taile', were used as the materials, and the walnut husk were inoculated with C. gloeosporioides, then the contents and related changes of phenolic compounds in walnut husk after infection were analyzed by targeted metabonomics. Result: With the increase of days after infection of C. gloeosporioides, the plaques on 'Xiangling' husk gradually increased and changed significantly on the 6th day, while the plaques on 'Taile' husk remained basically unchanged. The total amount of phenolic compounds in 'Xiangling' and 'Taile' husk was basically the same. The compounds were divided into 9 categories, among which were mainly procyanidins/anthocyanins, benzoic acid derivatives, flavonols and phenylpropanoids. 'Xiangling' had the most benzoic acids, accounting for more than 60%, and they were mainly gallic acid, syringic acid, ellagic acid and vanillic acid. While 'Taile' contained the most flavonols, nearly 30%, and they were mainly hyperin, quercitrin, avicularin and myricetin. On the 6th day of anthracnose infection, comparing with 'Xiangling', there were 60 different metabolites in 'Taile', among which52 different metabolites were up-regulated and 8 different metabolites were down-regulated. Among them, epicatechin 3-O-gallate, 4-Hydroxycinnamic acid, proanthocyanidin B1/2/3, phlorizin, syringic acid, ellagic acid and ferulic acid had significant differences. In addition, 47 substances such askaempferol and m-coumaric acid were not detected. On analysis of the differential metabolites in the 'Xiangling' and 'Taile' husk infected for 4-6 days with anthracnose, it was found that 6 compounds of caffeic acid, naringenin, eriodictyol, avicularin, quercetin and gallic acid changedobviously, which might be related to the significant changes in husk plaques. Conclusion: We have identified 130 phenolic compounds in the anthracnose resistant cultivar 'Taile' and the susceptible cultivar 'Xiangling'. Potential effective components against anthracnose, including proanthocyanidin B1/2/3, caffeic acid, naringenin, eriodictyol, avicularin, quercetin and gallic acid, have been screened by analyzing the dynamic changes of substances after anthracnose infection by using metabolomics. This study would provide references for the later development of anthracnose-related natural medicines and for exploring the mechanism of anthracnose.

Key words: metabonomics, walnut, anthracnose, phenolic compounds

CLC Number:

S718.4

Xin Chen,Min Wang,Maorun Fu,Guifang Wang,Kun Xiang,Qingzhong Liu,Wenxiao Jiao,Meiyong Zhang,Haifeng Xu. Metabolic Analysis of Phenolic Compounds Associated with Walnut Anthracnose[J]. Scientia Silvae Sinicae, 2021, 57(10): 71-80.

Figures/Tables 6

Fig.1

Fig.2

Fig.3

Fig.4

Table 1

Different metabolites between 'Xiangling' and 'Taile' husk after infection with C. gloeosporioides for 6 days ng·g-1"

酚类物质 Phenolic compounds	‘泰勒’青皮(A) ‘Taile’ husk	‘香玲’青皮(S) ‘Xiangling’husk	A_S FC
表儿茶素没食子酸酯(-)-Epicatechin gallate	280.14±23.18 a	5.65±0.73 b	49.56
对香豆酸p-Coumaric acid	2 216.28±179.74 a	45.30±6.00 b	48.93
原花青素B2 Procyanidin B2	5 719.79±426.19 a	145.06±11.95 b	39.43
原花青素B1 Procyanidin B1	7 103.90±891.17 a	186.64±16.57 b	38.06
异樱花亭Isosakuranetin	13.40±0.32 a	0.36±0.03 b	36.77
樱花亭Sakuranetin	13.49±0.54 a	0.43±0.04 b	31.52
原花青素B3 Procyanidin B3	3 572.87±195.48 a	126.54±27.47 b	28.24
根皮苷Phlorizin	125.84±11.15 a	6.05±0.26 b	20.80
柚皮素Naringenin	102.10±7.86 a	5.60±0.47 b	18.24
表没食子儿茶素没食子酸酯Epigallocatechin gallate	37.70±3.49 a	2.62±0.48 b	14.40
咖啡酸Caffeic acid	13 428.80±527.38 a	963.40±76.81 b	13.94
表儿茶素Epicatechin	3 425.07±298.76 a	301.48±11.72 b	11.36
单咖啡酰酒石酸Caftaric acid	397.97±8.72 a	37.78±2.66 b	10.54
(S)-圣草酚Eriodictyol	78.14±3.08 a	8.30±0.90 b	9.41
芹菜素-7-O-β-D-吡喃葡萄糖苷(大波斯菊苷)Cosmosiin	2.84±0.13 a	0.30±0.05 b	9.39
杨梅素-3-O-半乳糖苷Myricetin 3-O-galactoside	5 147.34±305.84 a	558.18±53.50 b	9.22
扁蓄苷Avicularin	19 733.39±974.32 a	2 186.02±318.06 b	9.03
阿福豆苷Afzelin	1 059.41±51.54 a	117.27±7.27 b	9.03
儿茶素Catechin	15 254.58±995.10 a	1 756.35±63.42 b	8.69
7-羟基香豆素7-Hydroxycoumarin	226.63±16.21 a	30.13±2.84 b	7.52
香橙素Aromadendrin	556.69±44.16 a	78.33±8.14 b	7.11
花旗松素Taxifolin	1 277.19±95.28 a	182.89±15.37 b	6.98
杨梅苷Myricitrin	10 536.44±1 078.14 a	1 573.40±131.69 b	6.70
木犀草素Luteolin	51.98±5.52 a	7.88±0.48 b	6.60
异鼠李素-3-O-葡萄糖苷Isorhamnetin-3-O-glucoside	52.41±2.96 a	8.03±1.24 b	6.53
表没食子儿茶素(-)-Epigallocatechin	228.95±16.17 a	37.11±2.90 b	6.17
槲皮素Quercetin	198.21±11.88 a	36.23±3.61 b	5.47
没食子儿茶素(±)-Gallocatechin	532.04±30.67 a	103.08±6.87 b	5.16
紫云英苷Astragalin	555.28±22.14 a	109.19±9.22 b	5.09
桑色素Morin	189.66±11.76 a	42.24±2.34 b	4.49
瑞香素Daphnetin	7 859.44±136.79 a	1 878.56±50.63 b	4.18
山柰酚-3-O-芸香糖苷Kaempferol 3-O-rutinoside	39.51±1.38 a	9.56±1.45 b	4.13
金丝桃苷Hyperoside	67 206.66±3 097.02 a	17 156.62±836.22 b	3.92
隐绿原酸Cryptochlorogenic acid	108 225.60±7 123.72 a	29 877.01±3 384.46 b	3.62
氯化飞燕草素-3-O-葡萄糖苷Delphinidin-3-O-glucoside chloride	90 358.19±4 157.00 a	25 354.43±3 534.47 b	3.56
槲皮素3-O-葡萄糖酸苷Quercetin 3-O-glucuronide	1 091.22±92.93 a	319.10±36.04 b	3.42
紫苏醇Perillyl alcohol	1 096.52±69.28 a	348.78±43.45 b	3.14
槲皮苷Quercitrin	54 309.70±3 669.72 a	17 416.75±1 489.66 b	3.12
对羟基苯甲酸p-Hydroxybenzoic acid	22 831.83±697.61 a	7 800.56±244.09 b	2.93
氯化矢车菊素-3-O-芸香糖苷Cyanidin 3-O-rutinoside chloride	37.76±2.32 a	12.99±1.50 b	2.91
松柏醛Coniferaldehyde	1 071.67±44.49 a	436.55±30.59 b	2.45
水仙苷Narcissin	39.33±2.78 a	16.16±1.49 b	2.43
异鼠李素Isorhamnetin	1.98±0.13 a	0.83±0.11 b	2.38
二氢杨梅素Dihydromyricetin	65.07±4.89 a	28.03±2.93 b	2.32
牡荆素Vitexin	6.16±0.66 a	2.70±0.35 b	2.28
3, 4-二羟基苯甲醛3, 4-Dihydroxybenzaldehyde	1 089.56±40.36 a	484.21±8.20 b	2.25
香兰素Vanillin	2 113.21±85.33 a	1 061.65±80.67 b	2.00
樱桃甙Prunin	351.77±33.00 a	175.70±8.32 b	2.00
没食子酸甲酯Methyl gallate	554.25±37.72 b	1 222.48±122.18 a	0.45
丁香酸Syringic acid	84 040.53±1 839.92 b	188 943.60±4 031.86 a	0.44
乔松素(S)-Pinocembrin	2.86±0.37 b	7.24±0.87 a	0.39
阿魏酸Ferulic acid	4 728.12±298.64 b	13 909.08±430.68 a	0.34
2, 6-二羟基苯甲酸2, 6-Dihydroxybenzoic acid	17.36±0.61 b	63.72±4.42 a	0.27
鞣花酸Ellagic acid	15 159.33±910.95 b	75 323.18±4 279.05 a	0.20
杨梅素Myricetin	49.57±2.00	0
原花青素A2 Proanthocyanidin A2	27.67±255	0
橙皮苷Hesperidin	15.19±0.75	0
根皮素Phloretin	8.01±0.46	0
刺芒柄花苷Ononin	0.18±0	0
1, 3-二咖啡酰奎宁酸(洋蓟素)1, 3-Dicaffeoylquinic acid	0	1.97±0.06
毛蕊异黄酮Calycosin	0	20.32±1.39

Table 1

Fig.5

References 0

	李敏, 刘媛, 孙翠, 等. 核桃营养价值研究进展. 中国粮油学报, 2009, 24 (6): 167- 170.
	Li M , Liu Y , Sun C , et al. Research progress on nutrient value of walnut. Journal of the Chinese Cereals and Oils Association, 2009, 24 (6): 167- 170.
	李晓娜, 曾小红, 谢龙莲, 等. 世界芒果炭疽病防治技术研究概况. 热带农业科学, 2017, (11): 69- 75.
	Li X N , Ceng X H , Xie L L , et al. The review on anthracnose prevention and control technology of mango. Chinese Journal of Tropical Agriculture, 2017, (11): 69- 75.
	柳凤, 詹儒林, 何衍彪, 等. 抑菌物质肉桂醛防治杧果炭疽病机制研究. 果树学报, 2011, 28 (4): 651- 656.
	Liu F , Zhan R L , He Y B , et al. Mechanism of biological control to anthracnoses in mango by antimicrobial cinnamaldehyde. Journal of Fruit Science, 2011, 28 (4): 651- 656.
	刘霞, 杨克强, 朱玉凤, 等. 8种杀菌剂对核桃炭疽病病原菌胶孢炭疽菌的室内毒力. 农药学学报, 2013, 15 (4): 412- 420. doi: 10.3969/j.issn.1008-7303.2013.04.08
	Liu X , Yang K Q , Zhu Y F , et al. Laboratory toxicity of eight fungicides against Colletotrichum gloeosporioides causing walnut anthracnose. Chinese Journal of Pesticide Science, 2013, 15 (4): 412- 420. doi: 10.3969/j.issn.1008-7303.2013.04.08
	陆俊, 赵安琪, 成策, 等. 核桃营养成分与生理活性及开发利用. 食品与机械, 2014, 30 (6): 238- 242.
	Lu J , Zhao A Q , Cheng C , et al. Nutrient composition, physiological activity, and development and utilization on walnut. Food & Machinery, 2014, 30 (6): 238- 242.
	马庆国, 乐佳兴, 宋晓波, 等. 新中国果树科学研究70年—核桃. 果树学报, 2019, 36 (10): 1360- 1368.
	Ma Q G , Le J X , Song X B , et al. Fruit scientific research in new China in the past 70 years: walnut. Journal of Fruit Science, 2019, 36 (10): 1360- 1368.
	米嘉琦, 王笑寒, 韩欣怡, 等. 肉桂醛对芒果胶孢炭疽病的抑制作用及其涂膜应用. 食品工业科技, 2020, 41 (5): 250- 256, 261.
	Mi J Q , Wang X H , Han X Y , et al. Inhibitory effect of cinnamaldehyde on mango anthracnose and its application of incorporating with wax. Science and Technology of Food Industry, 2020, 41 (5): 250- 256, 261.
	曲文文, 杨克强, 刘会香, 等. 山东省核桃主要病害及其综合防治. 植物保护, 2011, 37 (2): 136- 140. doi: 10.3969/j.issn.0529-1542.2011.02.030
	Qu W W , Yang K Q , Liu H X , et al. Main diseases of walnut and integrated management in Shandong. Plant Protection, 2011, 37 (2): 136- 140. doi: 10.3969/j.issn.0529-1542.2011.02.030
	万政敏. 2007. 核桃青皮中多酚类物质及其抗氧化性的分析. 呼和浩特: 内蒙古农业大学硕士学位论文.
	Wan Z M. 2007. Determination of the polyphenols and the antioxidant activity in the walnut green rind. Hohhot: MS thesis of Inner Mongolia Agricultural University. [in Chinese]
	汪爱娥. 2005. 不同寄主炭疽菌的生物学特性及辣椒抗性机制的研究. 合肥: 安徽农业大学硕士学位论文.
	Wang A E. 2005. Studies on biological characteristics of Colletotrichumspp from diverse hosts and the resistance mechanism of pepper to anthracnose. Hefei: MS thesis of Anhui Agricultural University. [in Chinese]
	杨光道, 段琳, 束庆龙, 等. 油茶果皮花青素、糖含量和PAL活性与炭疽病的关系. 林业科学, 2007, 43 (6): 100- 104.
	Yang G D , Duan L , Shu Q L , et al. Relationship of anthocyanin content, sugar content, PAL activity and Colletotrichum gloeosporioides in peel of oil tea tree. Scientia Silvae Sinicae, 2007, 43 (6): 100- 104.
	杨光道. 2009. 油茶品种对炭疽病的抗性机制研究. 合肥: 安徽农业大学硕士学位论文.
	Yang G D. 2009. Study on resistance of Camellia oleifera varieties to anthracnose. Hefei: MS thesis of Anhui Agricultural University. [in Chinese]
	姚瑞祺. 植物多酚的分类及生物活性的研究进展. 农产品加工, 2011, 4, 99- 100.
	Yao R Q . Research progress of plant polyphenols classification and biological function. Academic Periodical of Farm Products Processing, 2011, 4, 99- 100.
	余启明, 谢代祖, 蔡锦源, 等. 19种不同产地核桃的营养成分及脂肪酸的分析比较研究. 食品研究与开发, 2020, 41 (2): 149- 156.
	Yu Q M , Xie D Z , Cai J Y , et al. Analysis and comparison of the nutrients and fatty acids components in 19 walnuts(Juglans regia L.) from different regions. Food Research and Development, 2020, 41 (2): 149- 156.
	曾祥国, 张鹏, 韩永超, 等. 草莓新品种'晶玉'抗炭疽病相关的生理因子分析. 中国农学通报, 2016, 32 (34): 113- 118. doi: 10.11924/j.issn.1000-6850.casb16060025
	Zeng X G , Zhang P , Han Y C , et al. Physiological factors involved in resistance of strawberry cultivar'Jingyu'to anthracnose. Chinese Agricultural Science Bulletin, 2016, 32 (34): 113- 118. doi: 10.11924/j.issn.1000-6850.casb16060025
	赵梅. 2013. 梨果实对炭疸病和轮纹病抗性生理的研究. 南京: 南京农业大学硕士学位论文.
	Zhao M. 2013. Study on the resistance physiology against the pathogens of anthracnose and ring rot on pear fruit. Nanjing: MS thesis of Nanjing Agricultural University. [in Chinese]
	郑渝川, 王超, 程建伟, 等. 核桃青皮活性成分及分离纯化的研究进展. 四川林业科技, 2018, 39 (1): 22- 26.
	Zheng Y C , Wang C , Cheng J W , et al. Progress of research on the active ingredients, separation and purification of walnut green husk. Journal of Sichuan Forestry Science and Technology, 2018, 39 (1): 22- 26.
	Cosmulescu S N , Ion T , Achim G , et al. Phenolics of green husk in mature walnut fruits. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2010, 38 (1): 53- 56.
	Dicko M H , Gruppen H , Barro C , et al. Impact of phenolic compounds and related enzymes in sorghum varieties for resistance and susceptibility to biotic and abiotic stresses. Journal of Chemical Ecology, 2005, 31 (11): 2671- 2688. doi: 10.1007/s10886-005-7619-5
	Dowling M , Peres N , Villani S , et al. Managing Colletotrichum on fruit crops: a "complex" challenge. Plant Disease, 2020, 104, 2301- 2316. doi: 10.1094/PDIS-11-19-2378-FE
	Lima M C , Paiva D , Fernandez-Prada C , et al. A review of the current evidence of fruit phenolic compounds as potential antimicrobials against pathogenic bacteria. Microbial Pathogenesis, 2019, 130, 259- 270. doi: 10.1016/j.micpath.2019.03.025
	Lio D D , Cobodiaz J F , Masson C , et al. Combined metabarcoding and multi-locus approach for genetic characterization of Colletotrichum species associated with common walnut(Juglans regia)anthracnose in France. Scientific Reports, 2018, 8 (1): 10765- 10765. doi: 10.1038/s41598-018-29027-z
	Ma Y , Njike V Y , Millet J . Effects of walnut consumption on endothelial function in type 2 diabetic subjects. Diabetes Care, 2010, 33 (2): 227- 232. doi: 10.2337/dc09-1156
	Mikulic-Petkovsek M , Schmitzer V , Slatnar A , et al. Alteration of the content of primary and secondary metabolites in strawberry fruit by Colletotrichum nymphaeae infection. Journal of Agricultural & Food Chemistry, 2013, 61 (25): 5987- 5995.
	Oliveira I , Sousa A , Ferreira I C , et al. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food & Chemical Toxicology, 2008, 46 (7): 23- 26.
	Roy S , Nuckles E M , Archbold D D . Effects of phenolic compounds on growth of Colletotrichum spp. in vitro. Current Microbiology, 2018, 75, 550- 556. doi: 10.1007/s00284-017-1415-7
	Wang Q , Fan K , Li D , et al. Walnut anthracnose caused by Colletotrichum siamense in China. Australasian Plant Pathology, 2017, 46 (6): 585- 595. doi: 10.1007/s13313-017-0525-9
	Wang Q H , Fan K , Li D W , et al. Identification, virulence and fungicide sensitivity of Colletotrichum gloeosporioides s. s. responsible for walnut anthracnose disease in China. Plant Disease, 2020, 104, 1358- 1368. doi: 10.1094/PDIS-12-19-2569-RE
	Wang Q H , Li D , Duan C H , et al. First report of walnut anthracnose caused by Colletotrichum fructicola in China. Plant Disease, 2018, 102 (1): 247- 247.
	Zhu Y F , Yin Y F , Qu W W , et al. Morphological and molecular identification of Colletotrichum gloeosporioides causing walnut anthracnose in Shandong Province, China. Acta Horticulturae, 2014, 1050, 353- 359.

[1]	Xingzhou Chen,Guoying Zhou,Xinggang Chen,Lingyu Jiang,Anhua Bao,Jun Liu. Screening of Effectors of Colletotrichum fructicola in Camellia oleifera [J]. Scientia Silvae Sinicae, 2021, 57(9): 110-120.
[2]	Zhengrong Gong,Yifeng Wang,Han Wang,Wei Li,Mingjian Geng,Wenming Zhang,Lu Liu. Research Progress on Mineral Nutrition of Walnut [J]. Scientia Silvae Sinicae, 2021, 57(1): 178-190.
[3]	Lin Wang,Yongxin Dai,Jinsong Zhang,Ping Meng,Sheng Sun,Hao Li,Xianchong Wan. Effects of Water and Light Conditions on Photosynthesis and Growth of Soybean in Walnut-Soybean Agroforestry System [J]. Scientia Silvae Sinicae, 2020, 56(4): 188-196.
[4]	Li He, Li Sizheng, Wang Yuechen, Liu Jun, Xu Jianping, Zhou Guoying. Identification of the Pathogens Causing Anthracnose of Camellia oleifera in Nursery and Their Resistence to Fungicides [J]. Scientia Silvae Sinicae, 2019, 55(5): 85-94.
[5]	Li He, Li Yang, Jiang Shiqiang, Liu Jun'ang, Zhou Guoying. Pathogen of Oil-Tea Trees Anthracnose Caused by Colletotrichum spp. in Hunan Province [J]. Scientia Silvae Sinicae, 2017, 53(8): 43-53.
[6]	Li Shiliang, Ni Zhanglin, Mo Runhong, Qu Minghua, Tang Fubin, Liu Yihua. The Contents and Risk Assessments of Heavy Metals in Walnuts from the Main Producing Areas of Yunnan, Guizhou, Sichuan Provinces [J]. Scientia Silvae Sinicae, 2017, 53(11): 52-59.
[7]	Shen Yifan, Qian Jinfang, Zheng Xiaoping, Yuan Ziqian, Huang Jianqin, Wen Guosheng, Wu Jiasen. Spatial-Temporal Variation of Soil Fertility in Chinese Walnut (Carya cathayensis) Plantation [J]. Scientia Silvae Sinicae, 2016, 52(7): 1-12.
[8]	Zhang Junpei, Wang Zi, Zhou Xianwu, Zhao Rongjun, Xu Huige, Jia Zhiming, Pei Dong. Wood Physical and Mechanical Properties of American Black Walnut of Different Strains [J]. Scientia Silvae Sinicae, 2016, 52(6): 108-114.
[9]	Xiang Kun, Xu Ying, Li Guotian, Wang Xiaofang, Zhang Meiyong. Effects of Exogenous Nitric Oxide on Reactive Oxygen Metabolism of Walnut Seedlings under Low Temperature Stress [J]. Scientia Silvae Sinicae, 2016, 52(1): 143-149.
[10]	Liu Fangchun, Ma Hailin, Ma Bingyao, Du Zhenyu, Jing Dawei, Xing Shangjun. Effect of Plant Growth-Promoting Rhizobacteria on Photosynthetic Characteristics in Walnut Seedlings under Drought Stress [J]. Scientia Silvae Sinicae, 2015, 51(7): 84-90.
[11]	Yu Xianmei, Hou Changming, Wang Jie, Wang Hairong, An Miao, Ai Chengxiang. Identification of Pathogen Causing Beef Heart Persimmon Anthracnose in Shandong and Its Pathogenicity [J]. Scientia Silvae Sinicae, 2015, 51(4): 126-133.
[12]	Ren Junjie, Zhao Shuang, Li Meimei, Qi Guohui, Li Baoguo. Evaluation of Frost Resistance Ability of Different Walnut Cultivars [J]. Scientia Silvae Sinicae, 2014, 50(9): 67-72.
[13]	Li Wenjuan;Gao Haiyan;Tao Fei;Mu Honglei;Zhai Wenjing;. Effects of Storage Temperature and Initial Moisture Content on Oil Oxidation and Lipofuscin-like Pigments Accumulation of Walnut (Carya cathayensis) [J]. , 2013, 49(5): 62-70.
[14]	Cui Cui;Cai Jing;Zhang Shuoxin. Isolation and Identification of the Allelochemicals in Walnut (Juglans regia) Root Exudates [J]. , 2013, 49(2): 54-60.
[15]	Wu Nan;Liu Jun'ang;Zhou Guoying;Yan Ruikun;Dong Wentong. Inversion Anthracnose Disease Indices of Chinese Fir Based on Hyperspectral Derivative Indices [J]. Scientia Silvae Sinicae, 2012, 48(8): 94-98.

Metabolic Analysis of Phenolic Compounds Associated with Walnut Anthracnose

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 0

Related Articles 15

Recommended Articles

Metrics

Comments