Abstract:

triploids had been obtained by using different concentrations of colchicine solution and different methods for treating female flower buds of Populus tomentosa×P.bolleana,P.alba×P.glandulosa and P.abba×P.tomentosa.The results were as follows:①It was a moderate treating time for chromosome doubling of female gamete from female flower buds on the female flower branches which had been cultured in water for 1～5 days.This period of time was a sensitive phase of female gamete that was treated.② 3 methods of treating female flower buds with colchicine solution were effective.The better method was dipping female flower bud in the bottle of colchicine solution.③ The effective colchicine concentrations were 0.25 % and 0.50 %,the better one was 0.50 %.④The selected triploids had rapid growing,gigantic and vigorous properties at seedling period time.

Key words: White Poplar, Female gamete, Colchicine, Triploids