外源NO对低温胁迫下核桃幼苗活性氧代谢的影响

doi:10.11707/j.1001-7488.20160117

Abstract

Abstract: [Objective] Effects of exogenous nitric oxide(NO) on the active oxygen metabolism system of walnut seedlings under low temperature stress were studied, in order to investigate the possible mechanism of exogenous NO on the cold resistance of walnut.This study aimed at developing a new method for improving the resistance of fruit trees by applying exogenous NO and providing a theoretical basis for the wide application of NO on walnut in the future. [Method] The effects of sodium nitroprusside (SNP with 200 μmol·L^-1), a NO donor, on the active oxygen metabolism of walnut varieties ‘Xiangling’ and ‘Luguo No. 12’,seedlings with different cold resistance were studied in a artificial climate chamber. The seedling were subjected to low temperature treatments and the leaves were sprayed with SNP. [Result] 1) Under normal growth conditions, the effects of spraying SNP on the membrane permeability, chlorophyll content, production rate of superoxide anion radical(O₂^·-), H₂O₂, MDA and Pro content, and SOD and CAT activity in walnut leaves were not obvious. POD, DHAR, APX and GR activity, AsA and GSH content, GSH/GSSG and AsA/DHA ratio were significantly increased, while DHA and GSH content were reduced. The variation ranges of the two walnut varieties were different.2) Under low temperature stress, SNP treatment significantly increased the activity of POD, CAT, SOD, APX and GR, increased the content of chlorophyll, Pro, AsA, DHA, GSH and GSSG, and decreased the accumulation of MDA and H₂O₂, production rate of superoxide anion radical(O₂^·-) and relative permeability of plasma membrane. The variation ranges of the two walnut varieties were different. [Conclusion] Under low temperature stress, exogenous NO treatment could maintain the stability of AsA-GSH circulation system, decrease the accumulation of MDA and H₂O₂, production rate of superoxide anion radical(O₂^·-) and protect the stability of cell membrane structure by promoting antioxidant enzyme activity and antioxidant content, and there by reduce the damage of the active oxygen to the walnut leaf and enhance the cold resistance.

Key words: walnut, nitric oxide(NO), low temperature stress, active oxygen metabolism

CLC Number:

S718.43

Xiang Kun, Xu Ying, Li Guotian, Wang Xiaofang, Zhang Meiyong. Effects of Exogenous Nitric Oxide on Reactive Oxygen Metabolism of Walnut Seedlings under Low Temperature Stress[J]. Scientia Silvae Sinicae, 2016, 52(1): 143-149.

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Effects of Exogenous Nitric Oxide on Reactive Oxygen Metabolism of Walnut Seedlings under Low Temperature Stress

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