Welcome to visit Scientia Silvae Sinicae，Today is

Scientia Silvae Sinicae ›› 2015, Vol. 51 ›› Issue (4): 126-133.doi: 10.11707/j.1001-7488.20150416

Previous Articles     Next Articles

Identification of Pathogen Causing Beef Heart Persimmon Anthracnose in Shandong and Its Pathogenicity

Yu Xianmei1, Hou Changming2, Wang Jie1, Wang Hairong1, An Miao1, Ai Chengxiang1   

  1. 1. Shandong Institute of Pomology Tai'an 271000;
    2. Shandong Academy of Agricultural Sciences Ji'nan 250100
  • Received:2013-12-16 Revised:2014-07-02 Online:2015-04-25 Published:2015-05-20

PDF (PC)

605

Abstract:

【Objective】 This study aims to isolate and identify a pathogen which infects the persimmon plants and causes persimmon anthracnose, then investigate the pathogenicity so as to analyze its infect spectrum, and provide the theoretical basis and technical assistance for the pathogenic diagnosis and disease control of persimmon anthracnose in persimmon productivity.【Methods】 The pathogen was isolated from anthracnose lesions on beef heart persimmon fruits, leaves and twigs in the persimmon orchards in Zhujiapo town, Linju county, Zhuge town, Yishui county and Wanjishan experimental station of Shandong Institute of Pomology with tissue separation and single spore separation, and identified by morphological characteristics and rDNA-ITS sequence analysis. The pathogenicity was investigated via in vitro inoculation test by disk-wound inoculation, disk-no-wound inoculation and spore-suspension inoculation. 【Results】 Nine isolates were obtained from persimmon fruits, leaves and twigs via tissue separation and single spore separation. The analysis of morphological characteristics showed that the 9 isolates belong to genus Colletotrichum. Then rDNA-ITS sequence analysis, via PCR amplification with the universal primers of ITS6 and ITS4, showed that the 9 isolates obtained were in full accord with the morphological observation, and the nucleotide sequence of rDNA-ITS gene (GenBank accession No. KF010811) shared 100%, 100% and 99.8% identity with that of anthracnose pathogens on Jiro persimmon (JQ957543), 'Wuheshi' persimmon in Zhejiang (AY787483) and New Zealand isolate (GQ329690) respectively. The phylogenetic analysis based on ITS sequences showed that beef heart persimmon isolate was located at the same branch of phylogenetic tree with the isolates of Jiro persimmon (JQ957543), 'Wuheshi' persimmon in Zhejiang (AY787483, AY791890) and New Zealand persimmon (GQ329687, GQ329688 and GQ329690), which indicated that the pathogen causing the anthracnose disease of beef heart persimmon could be identified as Colletotrichum horii. The in vitro inoculation test showed that there was equal pathogenicity on different persimmon cultivars between 'Niuxinshi' isolate and 'Cilang' isolate of C.horii, and they have no pathogenicity on peach, apple and pear. On the 7th day after inoculation, the lesions on persimmon fruits were much larger than that on leaves, there were no symptoms on leaves via disk-no-wound inoculation, and lesions were small via spore suspension inoculation. The lesion size on fruits and leaves via different inoculation was inthe following order of:disk-wound inoculation > spore-suspension inoculation > disk-no-wound inoculation. 【Conclusions】C.horii possessed a certain degree of obligate parasitic and genetic conservatism, with no pathogenicity to other fruit trees besides persimmon and little variation due to the geographic distance. These characteristics were significant for the control of persimmon anthracnose, which could play an important role in avoiding and weakening the resistance to chemicals due to the genetic variation of the pathogen, and thus facilitate the control of persimmon anthracnose, and guarantee quality of fruits and increase persimmon productivity. The wound infection by the pathogen will occurred most easily, followed by spore infecton in raining days or under the high humidity condition, which indicated that the pathogen infection, the occurrence and popularity of persimmon anthracnose will be avoided or eliminated largely by reducing persimmon wound in the field management and avoiding pruning in raining days. Thus, the measures would to some extent solve the problem of lost production in persimmon due to the popularity of persimmon anthracnose.

Key words: beef heart persimmon, persimmon anthracnose, pathogen isolation and identification, rDNA-ITS sequence analysis, in vitro inoculation

CLC Number: