热胁迫对连翘离体叶圆片光系统Ⅱ活性的影响

doi:10.11707/j.1001-7488.20150406

Abstract

Abstract:

【Objective】In this study, we investigated the effects of high temperature on photosystemⅡkinetics activity and de-epoxidation level of xanthophyll cycle components in leaves of Forsythia suspensa to reveal the behavioral characteristics of photosynthetic apparatus under heat stress, and thereby enrich the research field on plant efficient utilization of light energy. 【Method】The leaf discs collected from near the midrib of F. suspensa leaves were used as the material and subjected to nine different temperature levels (26 ℃, 31 ℃, 34 ℃, 37 ℃, 40 ℃, 43 ℃, 46 ℃, 49 ℃ and 52 ℃) with three different treatment time levels (5 min, 25 min and 45 min). After the treatments, the leaf discs were maintained in darkness for 30 minutes at 26 ℃, and then their chlorophyll fluorescence parameters were measured using MINI-IMAGING-PAM system and their diffusing reflectance was tested by QE65 spectrometer, respectively. 【Result】The maximal photochemical efficiency (F_v/F_m) obviously decreased at 43 ℃, 37 ℃ and 37 ℃, respectively treated for 5min, 25min and 45min, while the actual photochemical efficiency (Y(Ⅱ)) decreased steadily, at 43℃, 31℃ and 31℃ under the heat stress time of 5 min, 25 min and 45 min. With the increase of treatment temperature and time, the minimum fluorescence of dark adaptation (F_o) and the photochemical fluorescence quenching coefficient (q_P) firstly increased and then decreased; The maximum fluorescence of dark adaptation (F_m) decreased significantly; The efficiency of excitation energy capture by open PSⅡreaction centers (F'_v/F'_m) and the electronic transport activity of PSII (F_m/F_o) reduced sharply; The sum of the quantum yield of regulated energy dissipation (Y(NPQ)) and the quantum yield of nonregulated energy dissipation (Y(NO)) increased with a trend to saturation; The relative deviation from full balance between PSⅡand PSⅠ(β/α-1) presented a sudden rise firstly and then sudden drop tendency. However the photochemical reflectance index (PRI) and Y(NO) showed the opposite trend.【Conclusion】The heat stress not only led to multiple sites injury of PSII protein complexes, but also destroyed the photosynthetic apparatus under a longer-time treatment with higher temperatures condition. In addition, the stress also turned the limiting factor from enzyme activity decay in dark reaction to PSⅡactivity decay. At the same time, the protection mechanisms against heat stress changed from Y(NPQ) to Y(NO) as the main form of energy dissipation, on the other hand, the protection mechanisms might also present by adjusting a balance of excitation energy between PSⅡand PSⅠin the form of state transition of antenna system.

Key words: heat stress, Forsythia suspense, chlorophyll fluorescence, photosystemⅡ, photosystemⅠ, xanthophyll cycle

CLC Number:

S718.43

Ren Zibei, Wang Junling, Shi Baosheng. Effects of Heat Stress on PhotosystemⅡActivity in Leaves of Forsythia suspensa[J]. Scientia Silvae Sinicae, 2015, 51(4): 44-51.

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Effects of Heat Stress on PhotosystemⅡActivity in Leaves of Forsythia suspensa

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