模拟酸雨不同处理对秃瓣杜英幼苗生物量和生理的影响

doi:10.11707/j.1001-7488.20160511

Abstract

Abstract:

[Objective] Comparisons of direct, indirect and composition effects of acid rain treatments on biomass allocation and physiological characteristics in Elaeocarpus glabripetalus seedlings were carried out in order to provide a scientific basis to study the mechanism of E. glabripetalus seedlings responding to simulated acid rain. [Method] Potted plants were used to establish the experiment, treatments included 2 different pH values of acid rain (pH2.5 and pH5.6) and 4 different models of exposure to acid rain, including 1) aboveground exposure: only the aboveground of seedlings were exposed to acid rain; 2) soil exposure: only the soil around seedlings was exposed to acid rain; 3)whole exposure: the seedlings and soil were both exposed to acid rain; 4) Control: exposed to distilled water with pH7.0. Biomass accumulation and allocation, chlorophyll content and chlorophyll fluorescence characteristics, malondialdehyde(MDA) content and membrane permeability, the activity of antioxidant enzymes were measured. [Result] 1)The growth of seedlings were inhabited to different extents when exposed to acid rain at pH2.5 level, and significant differences were found among the 3 models of exposure to acid rain. Compared with the control, total biomass, height increment, diameter increment of seedlings for the model of aboveground exposure decreased by 16.7%, 28.1% and 25.7% respectively; those of soil exposure decreased by 10.9%, 4.7% and 17.5% respectively; those of whole exposure decreased by 18.5%, 5.2% and 27.5% respectively. For the underground exposure, the ratio of leaf biomass to total biomass increased, and those of stem and root biomass decreased. The chlorophyll a (Chl a) content, total chlorophyll (total Chl) content, chlorophyll a/b(Chl a/b) were significantly lower than those of the control. For the aboveground exposure, primary photochemical efficiency of PSⅡ(F_v/F_m) and potential activity of PSⅡ(F_v/F_o) were significantly lower than those for the control; for the whole exposure the F_v/F_o and actual photochemical efficiency of PSⅡ (Φ_PS_Ⅱ) were significantly lower than those for the control; No significant differences were found between the soil exposure and the control in F_v/F_m, F_v/F_o and Φ_PS_Ⅱ. For all the 3 exposure models, membrane permeability, MDA content and antioxidant enzymes activity were significantly higher than those for the control, and those for the soil exposure were the highest. 2)At the pH5.6 level, there were no significant difference in biomass of seedlings between the 3 exposure models and the control, but Chl a content, total Chl content and Chl a/b decreased significantly, and membrane permeability, MDA content, peroxidase (POD), catalase (CAT) activity increased significantly compared with the control. For the aboveground exposure and the whole exposure, F_v/F_o and Φ_PS_Ⅱ were significantly lower than those for the control. [Conclusion] The soil exposure had both negative and positive effect on the seedlings, while the aboveground and whole exposures inhabited the seedlings, and the effect of whole exposure was greater than that of aboveground exposure, but the whole exposure did not change the biomass allocation of seedlings. The effect of acid rain at pH5.6 on seedlings was significantly lower than that of acid rain at pH2.5.

Key words: acid rain, Elaeocarpus glabripetalus, biomass allocation, chlorophyll fluorescence, membrane permeability, MDA, antioxidant enzyme activity

CLC Number:

S718.43

Yu Fei, Song Qi, Liu Meihua, Dong Lianchun, Yi Lita. Effects of Different Acid Rain Treatments on Biomass Allocation and Physiological Characteristics in Elaeocarpus glabripetalus Seedlings[J]. Scientia Silvae Sinicae, 2016, 52(5): 92-100.

References

陈娟, 曾青, 朱建国, 等. 2011. 施氮肥缓解臭氧对小麦光合作用和产量的影响. 植物生态学报, 35(5): 523-530.
(Chen J, Zeng Q, Zhu J G, et al. 2011. Nitrogen supply mitigates the effects of elevated[O₃] on photosynthesis and yield in wheat. Chinese Journal of Plant Ecology, 35(5): 523-530.[in Chinese])
高俊凤. 2006. 植物生理学实验指导. 北京: 高等教育出版社.
(Gao J F. 2006. Plant physiology experimental guidance. Beijing: Higher Education Press.[in Chinese])
郭春爱, 刘芳, 许晓明. 2006. 叶绿素b 缺失与植物的光合作用. 植物生理学通讯, 42(5): 967-973.
(Guo C A, Liu F, Xu X M. 2006. Chlorophyll-b deficient and photosynthesis in plants. Plant Physiology Communications,42(5): 967-973.[in Chinese])
孔繁翔, 刘营, 王连生,等. 1999. 酸沉降对马尾松菌根共生蛋白及营养关系影响. 环境科学, 20(6): 1-6.
(Kong F X, Liu Y, Wang L S, et al. 1999. Effects of artificial acid rain and different Ca/Al ratios on the symbiosis and nutrient relationship in mycorrhizae of Pinus massoniana. Environmental Science, 20(6): 1-6.[in Chinese])
李会合, 杨肖娥. 2009. 硫对超积累东南景天镉累积、亚细胞分布和化学形态的影响. 植物营养与肥料学报, 15(2): 395-402.
(Li H H, Yang X E. 2009. Effects of sulfur on accumulation, sub-cellular distribution and chemical forms of cadmium in hyperaccumulator-Sedum alfredii Hance. Plant Nutrition and Fertilizer Science,15(2): 395-402.[in Chinese])
李佳, 江洪, 余树全, 等. 2009. 模拟酸雨胁迫对青冈幼苗光合特性和叶绿素荧光参数的影响. 应用生态学报, 20(9): 2092-2096.
(Li J, Jiang H, Yu S Q, et al. 2009. Effects of simulated acid rain on Quercus glauca seedlings photosynthesis and chlorophyll fluorescence. Chinese Journal of Applied Ecology, 20(9): 2092-2096.[in Chinese])
灵君, 徐坤, 王磊, 等. 2013. 氮硫互作对越冬大葱生长及品质的影响. 植物营养与肥料学报, 19(5): 1272-1278.
(Ling J, Xu K, Wang L, et al. 2013. Influence of nitrogen and sulfur interaction on growth and quality of Chinese spring onion. Plant Nutrition and Fertilizer Science,19(5): 1272-1278.[in Chinese])
刘建芳, 周瑞莲, 赵梅, 等. 2011. 氮肥、钙肥和盐处理在冬小麦融冻胁迫适应中的生理调控作用. 生态学报, 31(23): 7161-7169.
(Liu J F, Zhou R L, Zhao M, et al. 2011. The role of the fertilizing with nitrogen, calcium and sodium chloride in winter wheat leaves adaptation to freezing-thaw stress. Acta Ecologica Sinica, 31(23): 7161-7169.[in Chinese])
刘可慧, 彭少麟, 莫江明, 等. 2005. 酸沉降对森林植物影响过程和机理. 生态环境, 14(6): 953-960.
(Liu K H, Peng S L, Mo J M, et al. 2005. The process and mechanism of rain deposition upon forest plants. Ecology and Environment,14(6): 953-960.[in Chinese])
路翔, 项文化, 刘聪. 2012. 中亚热带4种森林类型土壤有机碳氮贮量及分布特征. 水土保持学报,26(3):169-173.
(Lu X, Xiang W H, Liu C. 2012. Storage and distribution of soil organic carbon and nitrogen in four subtropical forests in central southern China. Journal of Soil and Water Conservation, 26(3):169-173.[in Chinese])
宋莉英, 柯展鸿, 孙兰兰, 等. 2013. 模拟酸雨对3种菊科入侵植物光合特性的影响. 植物学报, 48(2): 160-167.
(Song L Y, Ke Z H, Sun L L, et al. 2013. Effect of simulated acid rain on gas exchanges of three Compositae invasive plants. Bulletin of Botany, 48(2): 160-167.[in Chinese])
宋学贵, 胡庭兴, 鲜骏仁, 等. 2009. 川南天然常绿阔叶林土壤酶活性特征及其对模拟N沉降的响应. 生态学报, 29(3): 1234-1240.
(Song X G, Hu T X, Xian J R, et al. 2009. Soil enzyme activities and its response to simulated nitrogen deposition in an evergreen broad-leaved forest, southern Sichuan. Acta Ecologica Sinica, 29(3): 1234-1240.[in Chinese])
孙磊, 郝秀珍, 周东美, 等. 2014. 不同氮肥对污染土壤玉米生长和重金属Cu、Cd吸收的影响. 玉米科学, 22(3): 137-141.
(Sun L, Hao X Z, Zhou D M, et al. 2014. Effects of different nitrogen fertilizers on growth and heavy metals uptake by corn grown in contaminated soil. Journal of Maize Sciences, 22(3): 137-141.[in Chinese])
汪赛, 伊力塔, 余树全, 等. 2014. 模拟酸雨对青冈光合及叶绿素荧光参数的影响. 应用生态学报, 25(7): 2183-2192.
(Wang S, Yi L T, Yu S Q, et al. Effects of simulating acid rain on photosynthesis and chlorophyll fluorescence parameters of Quercus glauca. Chinese Journal of Applied Ecology, 25(7): 2183-2192.[in Chinese])
王利, 杨洪强, 张召, 等. 2011. 根区酸化对平邑甜茶叶片光系统Ⅱ活性及光合速率的影响. 林业科学, 47(10): 167-171.
(Wang L, Yang H Q, Zhang Z, et al. 2011. Effects of rhizosphere acidity on activity of photosystem Ⅱ and photosynthetic rate in Malus hupehensis leaves. Scientia Silvae Sinicae, 47(10): 167-171.[in Chinese])
解淑艳, 王瑞斌, 郑皓皓. 2012.2005-2011年全国酸雨状况分析. 环境监控与预警, 4(5): 33-37.
(Xie S Y, Wang R B, Zheng H H. 2012. Analysis on the acid rain from 2005 to 2011 in China. Environmental Monitoring and Forewarning, 4(5): 33-37.[in Chinese])
殷秀敏, 余树全, 江洪, 等. 2010. 酸雨胁迫对秃瓣杜英幼苗叶片叶绿素荧光特性和生长的影响. 应用生态学报, 21(6): 1374-1380.
(Yin X M, Yu S Q, Jiang H, et al. 2010. Effects of acid rain stress on Eleocarpus glabripetalus seedlings leaf chlorophyll fluorescence characteristics and growth. Chinese Journal of Applied Ecology, 21(6): 1374-1380.[in Chinese])
赵伟, 康丽莉, 林惠娟, 等. 2012. 临安大气本底站酸雨污染变化特征与影响因素分析. 中国环境监测, 28(4): 9-13.
(Zhao W, Kang L L, Lin H J, et al. 2012. Characteristics and affecting factors analysis of acid rain at Lin'an atmosphere watch regional station. Environmental Monitoring in China, 28(4): 9-13.[in Chinese])
中国科学院上海植物生理研究所. 1999. 现代植物生理学实验指南. 北京: 科学出版社.
(Shanghai Institutes for Plant Physiology, CAS. 1999. Modern plant physiology experimental manual. Beijing: Science Press.[in Chinese])
朱英华, 屠乃美, 肖汉乾, 等. 2008. 硫对烟草叶片光合特性和叶绿素荧光参数的影响. 生态学报, 28(3): 1000-1005.
(Zhu Y H, Tu N M, Xiao H Q, et al. 2008. Effects of sulfur nutrition on photosythesis and chlorophyll fluorescence of tobacco leaves. Acta Ecologica Sinica, 28(3): 1000-1005.)
邹琦. 2007. 植物生理学实验指导. 北京: 中国农业出版社.
(Zou Q. 2007. Plant physiology experimental guidence. Beijing: China Agriculture Press.[in Chinese])
Billen N, Schitzle H, Seufert G, et al. 1990. Performance of some growth variables. Environmental Pollution, 68(3/4): 419-434.
Cobbett C, Goldsbrough P. 2002. Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annual Review of Plant Biology, 53:159-182.
Genty B, Briantais J M, Baker N R. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta, 990(1):87-92.
Guo J H, Liu X J, Zhang Y, et al.2010.Significant acidification in major Chinese croplands.Science, 327(5968): 1008-1010.
Holland E A, Braswell B H, Lamarque J F, et al. 1997. Variations in the predicted spatial distribution of atmospheric nitrogen deposition and their impact on carbon uptake by terrestrial ecosystems. Journal of Geophysical Research: Atmospheres,102(D13):15849-15866.
Jacobson J S, Heller L I, Yamada K E, et al. 1990. Foliar injury and growth response of red spruce to sulfate and nitrate acidic mist. Canadian Journal of Forest Research, 20(1):58-65.
Knops J M, Reinhart K. 2000. Specific leaf area along a nitrogen fertilization gradient. American Midland Naturalist, 144(2): 265-272.
Ková D? ik J, Klejdus B, Ba D? kor M, et al. 2011. Physiological responses of root-less epiphytic plants to acid rain. Ecotoxicology, 20(2): 348-357.
Lebauer D S, Treseder K K. 2008. Nitrogen limitation of net primary productivity in terrestrial ecosystems is globally distributed. Ecology, 89(2):371-379.
Liu M H, Yi L T, Yu S Q, et al. 2015. Chlorophyll fluorescence characteristics and the growth response of Elaeocarpus glabripetalus to simulated acid rain. Photosynthetica, 53(1): 23-28.
Magill A H, Aber J D, Hendricks J J, et al. 1997. Biogeochemical response of forest ecosystems to simulated chronic nitrogen deposition. Ecological Applications, 7(2): 402-415.
Rausch T, Wachter A. 2005. Sulfur metabolism: a versatile platform for launching defence operations. Trends in Plant Science, 10(10): 503-509.
Rüdiger W. 2002. Biosynthesis of chlorophyll b and the chlorophyll cycle. Photosynthesis Research, 74(2): 187-193.
Schreiber U, Bilger W, Neubauer C. 1995. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. Ecophysiology of Photosynthesis, 100: 49-70.
Shukla J B, Sundar S, Naresh R. 2013. Modeling and analysis of the acid rain formation due to precipitation and its effect on plant species. Natural Resource Modeling, 26(1): 53-65.
Singh A, Agrawal M. 2008. Acid rain and its ecological consequences. Journal of Environmental Biology, 29(1): 15-24.
Solberg S, Dobbertin M, Reinds G J, et al. 2009. Analyses of the impact of changes in atmospheric deposition and climate on forest growth in European monitoring plots: a stand growth approach. Forest Ecology and Management, 258(8):1735-1750.
Sun Z G, Wang L H, Zhou Q, et al. 2013. Effects and mechanisms of the combined pollution of lanthanum and acid rain on the root phenotype of soybean seedlings. Chemosphere, 93(2): 344-352.
Thomas R Q, Canham C D, Weathers K C, et al. 2010. Increased tree carbon storage in response to nitrogen deposition in the US. Nature Geoscience, 3(1): 13-17.
Tomlinson G H. 2003. Acidic deposition, nutrient leaching and forest growth. Biogeochemistry, 65(1): 51-81.
Townsend A R, Braswell B H, Holland E A, et al. 1996. Spatial and temporal patterns in terrestrial carbon storage due to deposition of fossil fuel nitrogen. Ecological Applications, 6(3): 806-814.
Wang R L, Rehman S U, Liang X T, et al. 2012. Effects of simulated acid rain on the allelopathic potential of invasive weed Wedelia trilobata. Allelopathy Journal, 30(1):23-32.
Warren C R, Dreyer E, Adams M A. 2003. Photosynthesis-Rubisco relationships in foliage of Pinus sylvestris in response to nitrogen supply and the proposed role of Rubisco and amino acids as nitrogen stores. Trees, 17(4): 359-366.
Wen K J, Liang C J, Wang L H, et al. 2011. Combined effects of lanthanumion and acid rain on growth, photosynthesis and chloroplast ultrastructure in soybean seedlings. Chemosphere, 84(5): 601-608.
Wright I J, Reich P B, Westoby M, et al. 2004. The worldwide leaf economics spectrum. Nature, 428(6985): 821-827.

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Effects of Different Acid Rain Treatments on Biomass Allocation and Physiological Characteristics in Elaeocarpus glabripetalus Seedlings

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