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Scientia Silvae Sinicae ›› 2011, Vol. 47 ›› Issue (5): 23-30.doi: 10.11707/j.1001-7488.20110504

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A Genetic Frame Map of Salix suchowensis × S. erioclada Based on SSR and SRAP Markers

Liu Enying1,3, Wang Yuanxiu1,2, Xu Li1, Huang Minren1   

  1. 1. Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education Nanjing Forestry University Nanjing 210037;2. College of Life Sciences, Anhui Normal University Wuhu 241000;3. Forest Biotechnology Group, North Carolina State University Raleigh NC 27695 USA
  • Received:2010-01-28 Revised:2010-03-24 Online:2011-05-25 Published:2011-05-25

Abstract:

Based on a mapping population derived from Salix suchowensis × S. erioclada F1 family, a genetic frame map of willow with 117 markers (56 SSR, 54 SRAP, 5 SCAR and 2 sex markers) was constructed in this study. The map was totally 1 631.4 cM with average distance between markers of 14.1cM and covered 72.14% of the total length of the genome which was estimated to be as long as 2 261.39 cM. Two sex markers were located on group 1 and 17 and the marker SCAR_AE08-780-5 was co-segregated with the female marker Sex-F on group 1. Alignment of this frame map to the Populus trichocarpa physical map revealed 46 homologous markers, 15 homological linkage groups and 34 of 42 homologous markers (80.95%) were at high co-linearity. Overall, the genome of the Salicaceae family was well conserved in the course of evolution.

Key words: Salix suchowensis, Salix erioclada, SRAP, SSR, genetic linkage map

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