感染油茶象幼虫的高致病力金龟子绿僵菌菌株筛选

doi:10.11707/j.1001-7488.20150807

Abstract

Abstract:

[Objective] Curculio chinensis (Coleoptera: Curculionidae) is one of the most serious pests of oil-tea Camellia fruits.Screening of Metarhizium anisopliae strains with high virulence against this pest is important for using fungi as biocontrol agent to suppress this pest.[Method] Colony growth and sporulation of 7 strains of M. anisopliae were measured and their virulence against C. chinensis larvae were investigated in laboratory by using two inoculating methods ( Being dipped on larvae with conidia suspension of 10⁷ spores·mL^-1and being mixed in soil with 10⁵spores·g^-1). The infection effects in field were evaluated through spraying conidia suspension of two superior strains. [Result] Bioassay results showed that larvae of C. chinensis were susceptible to different M. anisopliae strains. The larval mortalities reached 100% on the 13th day after inoculation using dipping inoculative method and the 9th day for conidia-soil mixing inoculative method. The median lethal time (LT₅₀) of dipping inoculative method was 1.65 days to 4.26 days, and that of conidia-soil mixing inoculative method was 1.96 days to 3.51 days. The FJMa201101 and FJMa201205 strains of M. anisopliae were the most virulent against larvae of C. chinensis. When larvae inoculated with FJMa201101, the LT₅₀ was 1.65 days, and the infection rate was 86.6% when using dipping inoculative method, while the LT₅₀ was 2.11 days and the infection rate was 94.5% respectively for conidia-soil mixing inoculative method. When inoculated with FJMa201205, the LT₅₀ was 1.71 days with the infection rate of 91.1% by using dipping inoculative method, whilst it was 1.96 days with the infection rate of 88.9% with conidia-soil mixing inoculative method. In field test, there was no significant difference in infection rate between the two superior strains, but the infection rates of larvae introduced to soil after spraying conidia suspension were significantly higher than those introduced before spraying. Meanwhile, the two strains were excellent at growth performance. [Conclusion] Therefore, the two strains of M. anisopliae, FJMa201101 and FJMa201205, could be the promising candidate in controlling C. chinensis larvae.

Key words: Curculio chinensis, Metarhizium anisopliae, virulence, dipping inoculative method, conidia-soil mixing inoculative method, pest of oil-tea Camellia fruit

CLC Number:

S763.306

He Xueyou, Cai Shouping, Du Yuefei, Chen Delan, Huang Jinshui, Li Kongquan. Screening of Metarhizium anisopliae Strain with High Virulence against Larvae of Curculio chinensis (Coleoptera: Curculionidae)[J]. Scientia Silvae Sinicae, 2015, 51(8): 52-59.

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Screening of Metarhizium anisopliae Strain with High Virulence against Larvae of Curculio chinensis (Coleoptera: Curculionidae)

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