橡胶树水通道蛋白基因HbPIP1和HbPIP2的功能鉴定及其表达分析

doi:10.11707/j.1001-7488.20140111

Abstract

Abstract:

In this study, Xenopus laevis oocytes were used to test the functional characterization of our previously cloned HbPIP 1 and HbPIP 2 aquaporin genes from rubber trees (Hevea brasiliensis). The result showed that HbPIP 1 had the function of transmembrane water transport however HbPIP 2 has not. We therefore infer that HbPIP 1 is involved in the mechanism of Ethrel-induced latex promotion. An Ethrel sensitive rubber clone PR107 was selected as the plant material. The gene expression profiles of HbPIP 1 at both transcript level and translation level were examined by RT-qPCR and Western blot to investigate their link with the effect of Ethrel on latex flow duration, latex volume, dry rubber content (DRC) and total solids content (TSC). It was found that the expression of HbPIP 1 in latex, bark and xylem was higher than that in petioles and blades of various periods. The expression of HbPIP 1 in PR107 latex presented an increase trend with the Ethrel treatment duration, while it was down-regulated in the bark. This expression might facilitate the water transport into rubber laticifers and therefore can decrease latex DRC and TSC, prolong latex flow and enhance the latex yield.

Key words: Hevea brasiliensis, aquaporin, function characterization, expression analysis, tissue-specific expression, Ethrel stimulation, latex dilution

CLC Number:

S718.46

Wang Jin, An Feng, Cai Xiuqing, Zou Zhi, Zhang Wei, Lin Weifu. Functional Characterization and Expression Analysis of Aquaporin Genes (HbPIP 1 and HbPIP 2) in Hevea brasiliensis[J]. Scientia Silvae Sinicae, 2014, 50(1): 69-75.

References

黄昊, 苏维埃. 1998. 植物水孔蛋白基因PIP 1 a在爪蟾卵母细胞中的表达和功能测定. 植物生理学通讯, 34(6):448-450.
(Huang H, Su W A. 1998. Expression and functional detection of plant AQPs gene PIP1a in Xenopus oocytes. Plant Physiology Communications, 34(6):448-450. [in Chinese] )
李红梅, 万小荣, 何生根. 2010. 植物水孔蛋白最新研究进展. 生物化学与生物物理进展, 37(1): 29-35.
(Li H M, Wan X R, He S G. 2010. Advances in plant aquaporins. Progress in Biochemistry and Biophysics, 37(1): 29-35. [in Chinese] )
李明, 罗世巧, 刘实忠. 2010. 乙烯刺激橡胶树胶乳黄色体蛋白差异初析. 热带作物学报, 31(3):371-375.
(Li M, Luo S Q, Liu S Z. 2010. Differential expression of Hevea lutoid protein under ethylene stimulation. Chinese Journal of Tropical Crops, 31(3):371-375. [in Chinese] )
阮想梅, 李登弟, 李学宝. 2009. 植物水孔蛋白的功能和调控. 植物生理学通讯, 45(1):1-7.
(Ruan X M, Li D D, Li X B. 209. Function and regulation of plant aquaporin. Plant Physiology Communications, 45(1):1-7. [in Chinese] )
肖再云, 刘实忠, 校现周. 2010. 橡胶树PR107气刺微割排胶影响面胶乳生理分析. 林业科学, 46(9):65-72.
(Xiao Z Y, Liu S Z, Xiao X Z. 2010. Analysis on physiological parameters of latex in flow area on rubber tree(Hevea brasiliensis) clone PR107 by minicut with gaseous stimulation. Scientia Silvae Sinicae, 46(9):65-72. [in Chinese] )
徐智娟, 袁坤, 丁璇, 等. 2010. 橡胶树胶乳全蛋白的提取及双向电泳分析. 热带农业科学, 30(4):1-4.
(Xu Z J, Yuan K, Ding X, et al. 2010. The extration and two-dimensional electrophoresis analysis of total proteins from the rubber tree latex. Chinese Journal of Tropical Agriculture, 30(4):1-4. [in Chinese] )
杨华庚. 2010. 橡胶树割胶原理与技术. 海口:海南出版社.
郑亚军, 李艳, 李新菊, 等. 2008. 不同SDS-PAGE分离胶浓度下椰子贮藏蛋白质亚基的分离效果. 中国农学通报, 24(9):452-456.
(Zheng Y J, Li Y, Li X J, et al. 2008. The effect of coconut storage protein subunits under different separation gel concentrations of SDS-PAGE. Chinese Agricultural Science Bulletin ,24(9):452-456. [in Chinese] )
庄海燕, 安锋, 何哲, 等. 2010. 巴西橡胶树水通道蛋白基因cDNA的克隆及序列分析. 西北植物学报, 30(5):861-868.
(Zhuang H Y, An F, He X, et al. 2010. Molecular cloning and sequence analysis of cDNAs encoding aquaporins in Hevea brasiliensis. Acta Botanica Boreali-Occidentalia Sinica, 30(5):861-868. [in Chinese] )
Coupe M, Lambert C, D'Auzac J. 1976. Etude comparative des polyribosomes du latex d'Hevea sous l'action de l'ethrel et d'autres produits augmentant l'ecoulement du latex. Physiol Veg, (14):391-406.
Damerval C, Dervienne D, Zivy M, et al. 1986. Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat seedlings proteins. Electrophoresis, 7(1):52-54.
Fetter K, Van Wilder V, Moshelion M, et al. 2004. Interactions between plasma membrane aquaporins modulate their water channel activity. The Plant Cell, (16):215-228.
Gomez J B. 1977. Demonstration of latex coagulants in bark extracts of Hevea and their possible role in latex flow. J Rubb Res Inst Malaysia, 25(3):109-119.
Hill A E, Shachar-Hill B, Shachar-Hill Y. 2004. What are aquaporins for?. J Membr Biol, 197(1): 1-32.
Horie T, Kaneko T, Sugimoto G. 2011. Mechanisms of water transport mediated by PIP aquaporins and their regulation via phosphorylation events under salinity stress in barley roots. Plant and Cell Physiology, 52(4):663 -675.
Jones J D, Dangl J L. 2006. The plant immune system. Nature, 444(7117): 323-329.
Lecourieux D, Ranjeva R, Pugin A. 2006. Calcium in plant defence-signalling pathways. New Phytologist, 171(2): 249-269.
Li H, Qin Y, Xiao X, et al. 2011. Screening of valid reference genes for real-time RT-PCR data normalization in Hevea brasiliensis and expression validation of a sucrose transporter gene HbSUT 3. Plant Sci, 181(2): 132-139.
Maurel C, Verdoucq L, Luu D T, et al. 2008. Plant aquaporins: membrane channels with multiple integrated functions. Annual Review of Plant Biology, (59): 595-624.
Ribaillier D. 1968. Action in vitro de certain ions mine raux et composes organiques surla stabilite des lutoids du latex d'Hevea. Rev Gen Caout Plast, 45(12): 1395-1398.
Shapiguzov A Y. 2004. Aquaporins: structure, systematics, and regulatory features. Russian Journal of Plant Physiology, 51 (1):127-137.
Tang C R, Qi J Y, Li H P, et al. 2007. A convenient and efficient protocol for isolating high-quality RNA from latex of Hevea brasiliensis (para rubber tree). Journal of Biochemical and Biophysical Methods, 70(5): 749-754.
Tungngoen K, Soulaiman S, Kongsawadworakul P, et al. 2006. Aquaporin genes expression in the trunk phloem and in the laticifers of rubber tree. Ho Chi Minh City, Vietnam: IRRDB Annual Meeting and Conference.
Tungngoen K, Kongsawadworakul P, Viboonjun U, et al. 2009. Involvement of HbPIP2;1 and HbTIP1;1 aquaporins in ethylene stimulation of latex yield, through regulation of water exchanges between inner liber and latex cells in Hevea brasiliensis. Plant Physiology, 151(2): 843-856.
Yip E, Chin H C. 1977. Latex flow studies: Distribution of metallic ions between phase of Hevea latex and effect of yield stimulation on the distribution. J Rubb Res lnst Malaysia, 25(1):31-49.

[1]	Tang Xin, Wang Ruihui, Yang Xiuyan, Zhu Jianfeng, Liu Zhengxiang, Ni Jianwei, Zhang Huaxing. Isolation and Expression Analysis of a Vacuolar Membrane Na⁺/H⁺ Antiporter Gene NtNHX1 from Nitraria tangutorum [J]. Scientia Silvae Sinicae, 2014, 50(3): 38-44.
[2]	Li Changjiang, Sun Fan, Zhang Tong, Zhang Lingyun. Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii [J]. Scientia Silvae Sinicae, 2013, 49(10): 40-47.
[3]	Cui Qinqin;Han Xiaojiao;Chen Yicun;Zhan Zhiyong;Lin Liyuan;Wang Yangdong. Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene(VfBCCP) from Vernicia fordii [J]. Scientia Silvae Sinicae, 2012, 48(8): 155-160.
[4]	Ren Lei;Wang Yan;Zhou Lin;Peng Zhenhua. Isolation and Expression of PsAP 2 Gene in Tree Peony [J]. Scientia Silvae Sinicae, 2011, 47(9): 50-56.
[5]	Li Linling;Cheng Hua;Xu Feng;Wang Yan;Jiang Dezhi;Cheng Shuiyuan. Isolation and Expression of the Chloroplast Copper/Zinc Superoxide Dismutase Gene (GbCuZnSOD ) from Ginkgo biloba [J]. Scientia Silvae Sinicae, 2010, 46(6): 35-42.
[6]	Su Hongyan;Wang Lei;Wang Zhongli;Feng Peiyong;Zhang Ye. Cloning and Characterization of PeMYBL1, an R2R3 MYB Gene from Poplar [J]. Scientia Silvae Sinicae, 2010, 46(1): 142-146.
[7]	Hu Xiaoyi;Tan Xiaofeng;Tian Xiaoming;Liu Qiao;LuoQian;Chen Hongpeng;Hu Fangming. Identification and Analysis of an Aquaporin(CoPIP1-1) in the Seeds of Camellia oleifera [J]. Scientia Silvae Sinicae, 2008, 44(12): 48-56.
[8]	Zhou Zhou;Zhang Deqiang;Lu Mengzhu. Cloning and Expression Analysis of PtFAD2 Gene Encoding the Endoplasmic Reticulum Fatty Acid 18∶1 Desaturase in Populus tomentosa [J]. Scientia Silvae Sinicae, 2007, 43(07): 16-21.

Functional Characterization and Expression Analysis of Aquaporin Genes (HbPIP 1 and HbPIP 2) in Hevea brasiliensis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments