Abstract:

Embryo rescue of interspecific hybrids between wild diploid species Dendranthema lavandulifolium,D.nankingense,D.dichrum and cultivated species D.morifolium was studied in this paper.The results indicated that the genotypes and the days of embryo rescue were important factors.13～18 days after pollination were the suitable time for embryo rescue.Medium had little effect on ovary culture.Beneficial proportion of hormones was BA 2.0 mg·L^-1+NAA 2.0 mg·L^-1.The rescue result was better when wild diploid species used as female parents than as male parents.RAPD and morphological analysis showed that they were real hybrids.

Key words: Dendranthema, wild diploid species, cultivated species(Dendranthema morifolium), interspecific hybrid, embryo rescue, RAPD