落羽杉属树种及其杂交后代亲缘关系的RAPD分析

doi:10.11707/j.1001-7488.20070209

Scientia Silvae Sinicae ›› 2007, Vol. 43 ›› Issue (02): 48-51.doi: 10.11707/j.1001-7488.20070209

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Genetic Relationships of Taxodium Species and Their Hybrids Based on RAPD Analysis

Li Han^1,2 Yin Yunlong¹ Xu Langlai² Yu Chaoguang¹ Chen Yonghui¹

1.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences Nanjing 210014;2.College of Life Sciences,Nanjing Agriculture University Nanjing 210095

Received:2005-12-07 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

Abstract

Abstract:

The genetic relationship of thirteen species and hybrids of Taxodium was analyzed by random amplified polymorphic DNA(RAPD). Thirteen better primers were selected from one hundred arbitrary 10 bp primers. Total 92 bands were produced, with which 72 were polymorphic. According to the cluster analysis, the results showed that the genetic relationship of first crossed generation (F₁) Taxodium ‘Zhongshansha 302'(T.distichum×T.mucronatum)was closer to the female parent T. distichum. Among the hybrids of first backcrossed generations(BCF₁), the genetic similarity coefficients of RAPD between BCF₁118 and BCF₁140 was the highest, and BCF₁1 was closer to the female parent Taxodium ‘Zhongshansha 302’, while BCF₁118 was closer to the male parent T. mucronatum.

Key words: Taxodium, hybrids, RAPD, genetic relationship

Li Han;Yin Yunlong Xu Langlai Yu Chaoguang Chen Yonghui. Genetic Relationships of Taxodium Species and Their Hybrids Based on RAPD Analysis[J]. Scientia Silvae Sinicae, 2007, 43(02): 48-51.

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Genetic Relationships of Taxodium Species and Their Hybrids Based on RAPD Analysis

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