23个油橄榄品种的RAPD分析*

doi:10.11707/j.1001-7488.20080115

Scientia Silvae Sinicae ›› 2008, Vol. 44 ›› Issue (1): 85-89.doi: 10.11707/j.1001-7488.20080115

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RAPD Analysis of 23 Olive (Olea europaea) Cultivars

Qiu Yuan^1.2,Han Huabo³,Li Junqiang⁴,Hou Chunxia¹,Wang Yongqing¹

(1.College of Forestry and Horticulture, Sichuan Agricultural University Ya'an 625014； 2.Zigong Agricultural Bureau, Sichuan Province Zigong 643000; 3.Sichuan Academy of Forestry Chengdu 610081; 4Yibin Vocational and Technical College, Sichuan Province Yibin 644003)

Received:2006-10-16 Revised:1900-01-01 Online:2008-01-25 Published:2008-01-25

Abstract

Abstract:

Random amplified polymorphic DNA (RAPD) was used to study the classification and identification of 23 olive (Olea europaea) cultivars. Eleven 10 bp primers selected from eighty arbitrary primers were applied for amplifying the olive DNA. Total 127 bands were produced, among which 78 bands (61.4%) were polymorphic. The average numbers of polymorphic DNA bands amplified by each primer were 7.09 There were specific bands in four cultivars which can be used in olive germplasm identification. A dendrogram showing genetic relationships was constructed through an unweighted pairgroup method （UPGMA） based on the DNA polymorphism and the 23 cultivars were clustered into 2 main groups.

Key words: olive (Olea europaea), RAPD analysis, cultivar classification, genetic diversity

Qiu Yuan.;Han Huabo;Li Junqiang;Hou Chunxia;Wang Yongqing. RAPD Analysis of 23 Olive (Olea europaea) Cultivars [J]. Scientia Silvae Sinicae, 2008, 44(1): 85-89.

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RAPD Analysis of 23 Olive (Olea europaea) Cultivars

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