无花果叶形性状的SCAR分子标记*

doi:10.11707/j.1001-7488.20090628

Scientia Silvae Sinicae ›› 2009, Vol. 12 ›› Issue (6): 158-161.doi: 10.11707/j.1001-7488.20090628

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A SCAR Molecular Marker Related to Leaf Shape of Fig （Ficus carica）

Wang Liang,Wang Caihong,Tian Yike,He Xiaowei,Wang Hui

（College of Horticulture, Qingdao Agricultural University Qingdao 266109）

Received:2008-05-14 Revised:1900-01-01 Online:2009-06-25 Published:2009-06-25

Abstract

Abstract:

A RAPD marker specific to leaf shape of fig (Ficus carica) was developed and converted into SCAR marker for germplasm identification. RAPD amplifications with random primers S-2082 produced a unique fragment linking to the noncordate base leaf shape. The unique fragment was extracted, cloned and sequenced. Sequencing results indicated that the size of the fragment was 1 640 bp. Specific primers were designed based on its sequence and used in SCAR-PCR, and it was showed that the RAPD marker from S2082-1640 was successfully converted into SCAR marker.

Key words: Fig (Ficus carica), leaf shape, RAPD, SCAR

Wang Liang;Wang Caihong;Tian Yike;He Xiaowei;Wang Hui. A SCAR Molecular Marker Related to Leaf Shape of Fig （Ficus carica）[J]. Scientia Silvae Sinicae, 2009, 12(6): 158-161.

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A SCAR Molecular Marker Related to Leaf Shape of Fig （Ficus carica）

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