Abstract:

RAPD markers was used to study the phylogenetic relationship among Chinese wild species and cultivars of Paeonia sect. Moutan. 18 primers selected from 210 decamer primers with clear and reproducible patterns generated 153 polymorphic bands(80.1%) among a total of 191 that revealed abundant genetic diversity among sect. Moutan. The number of the RAPD bands was recorded for every accession and 25 special makers could promote classification and identification of sect. Moutan. The cluster analysis based on the RAPD results was performed by UPGMA. The 23 OTUs (Operational taxonomic units) were divided into 3 groups. 5 wild species ( P. rockii, P. jishanensis, P. ostii, P. decomposita, P. qiui) and 11 cultivars got into one group. The genetic distances between every wild species and cultivars were also calculated, and the sequence based on genetic distance (0.48～0.57) from big to small was as follows: P. ostii, P. decomposita, P. qiui, P. rockii and P. jishanensis.

Key words: Paeonia sect. Moutan, Phylogenetic relationship, RAPD