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林业科学 ›› 2023, Vol. 59 ›› Issue (12): 105-116.doi: 10.11707/j.1001-7488.LYKX20220420

• 研究论文 • 上一篇    下一篇

强割和乙烯利刺激对不同品系幼龄橡胶树排胶生理特性的影响

丁欢,杨署光,蒋毅,葛立鑫,田维敏,史敏晶*   

  1. 中国热带农业科学院橡胶研究所 农业农村部橡胶树生物学与遗传资源利用重点实验室 省部共建国家重点实验室培育基地?海南省热带作物栽培生理学重点实验室 海口 571101
  • 收稿日期:2022-06-20 出版日期:2023-12-25 发布日期:2024-01-08
  • 通讯作者: 史敏晶
  • 基金资助:
    海南省基金创新团队项目(320CXTD442);国家重点研发计划课题(2022YFD23012015);国家自然科学基金项目(31870590);现代农业产业技术体系建设专项(CARS-33-YZ1)。

Effects of Intensive Tapping and Ethrel Stimulation on the Physiological Characteristics of Young Rubber Trees of Different Varieties During Latex Flow

Huan Ding,Shuguang Yang,Yi Jiang,Lixin Ge,Weimin Tian,Minjing Shi*   

  1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences  Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs   State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops Haikou 571101
  • Received:2022-06-20 Online:2023-12-25 Published:2024-01-08
  • Contact: Minjing Shi

摘要:

目的: 研究不同割胶方式下不同品系幼龄橡胶树的排胶生理特性,为阐述乳管排胶机制提供理论基础,也为天然橡胶生产中针对不同品系进行合理规划割胶提供参考依据。方法: 选取PR107、RY8-79、Tjir1和RY7-33-97共4个品系的6龄未开割橡胶树,在S/2 d/2(割线长度为1/2茎围,每2天割1刀)的强割和后续添加1%乙烯利刺激割胶方式下,对其橡胶粒子粒径以及干胶含量、排胶体积、排胶时间、排胶初速度、堵塞指数、硫醇含量等常规排胶生理参数进行分析,并比较不同割胶刀次对这些参数的影响。结果: 1) 不同品系胶乳橡胶粒子粒径具有明显差异,起始值最大的是RY8-79和PR107,RY7-33-97 的胶乳粒径较小,Tjir1的胶乳粒径最小。强割后,随着割胶刀次增加,不同品系胶乳粒径普遍短暂上升后降低,其中RY8-79和 Tjir1的胶乳粒径变化较大,PR107 和RY7-33-97的胶乳粒径相对稳定,但均在强割8刀后胶乳粒径降至0.80 μm以下,与最大胶乳粒径之间存在显著差异(P<0.05)。乙烯利刺激后,胶乳粒径维持在较小水平。2) 干胶含量起始值最高为RY7-33-97,其次为RY8-79和PR107,最差为Tjir1。在强割条件下,RY8-79和RY7-33-97干胶含量下降较快,PR107和Tjir1干胶含量下降相对缓慢;乙烯利处理后,PR107干胶含量仅略有下降;RY8-79、RY7-33-97和 Tjir1干胶含量相对下调趋势略明显,尤其是RY7-33-97。3) 强割条件下,不同品系的排胶时间和排胶体积变化规律高度一致,RY8-79排胶总体积和时间明显高于PR107、RY7-33-97和Tjir1;乙烯利处理后所有品系的排胶时间和总体积显著提高(P<0.05),其中PR107和Tjir1这2个排胶少的品系受乙烯利刺激的影响更明显。4) 强割条件下RY8-79和RY7-33-97排胶初速度明显高于PR107和Tjir1,但乙烯利刺激显著降低所有品系的排胶初速度。5) 强割条件下不同品系堵塞指数差异较大,RY8-79最低,RY7-33-97最高,乙烯利刺激明显降低该指数。6) 强割条件下RY8-79硫醇含量最高,其次为RY7-33-97、PR107,Tjir1最低,乙烯利刺激后48 h 4个品系硫醇含量均表现出降低趋势。结论: 不同品系对强割和乙烯利刺激后的排胶反应不同。强割和过度乙烯利刺激均不利于橡胶粒子粒径、干胶含量等产排胶相关参数的恢复。橡胶粒子粒径和干胶含量相对其他参数受周边环境影响较小,可作为衡量排胶是否过度的参考指标。橡胶粒子粒径0.80 μm是一个较有标志性的值,胶乳粒径降到该值之下,预示应降低割胶强度。自然条件下排胶量大的品系通常不耐强割和乙烯利刺激,RY8-79和RY7-33-97作为主栽品种应尽量避免割胶中施加乙烯利,而PR107可配合施用乙烯利刺激剂。利用幼龄树,结合强割和乙烯利刺激,可以作为育种早期快速筛选不同品系是否具有耐割和耐刺激的方法之一,也为不同品系割胶制度的制定提供依据。

关键词: 橡胶树, 强割, 乙烯利, 橡胶粒子, 排胶

Abstract:

Objective: In this paper, the physiological characteristics of latex flow in young rubber trees of different varieties under the various tapping systems were studied, in order to lay the theoretical foundation for elaborating the mechanism of latex flow and provide a guidance for planning rational tapping system to different varieties in natural rubber production. Method: The 6-year-old healthy virgin rubber trees of 4 varieties, PR107, RY8-79, Tjir1 and RY7-33-97, were selected, and the intensive tapping with S/2 d/2 (tapping line length of ? stem circumference and one tapping every two days) and subsequent stimulation with 1% ethrel were applied. The rubber particle size, and conventional physiological parameters of latex flow, such as the dry rubber content (DRC), latex volume, duration of latex flow, initial velocity and plugging index, were investigated, and further the effects of different tapping times were compared. Result: 1) The rubber particle size of different varieties was different obviously. In the virgin tree, the order of rubber particle size was RY8-79 >PR107 > RY7-33-97 > Tjir1. During the intensive tapping, the rubber particle size of different varieties increased firstly then decreased with the increase in tapping times, and the rubber particle size of RY8-79 and Tjir1 changed faster than that of PR107 and RY7-33-97. After the eighth intensive tapping, the rubber particle size decreased to below 0.80 μm usually, which was significantly lower than the largest size (P<0.05). During the ethrel stimulation, rubber particle size generally showed a downward trend and remained at a low level. 2) In the virgin tree, the order of DRC was RY7-33-97>RY8-79 >PR107 >Tjir1. Under the intensive tapping, DRC of RY8-79 and Ry7-33-97 dropped faster than that of PR107 and Tjir1. During the ethrel treatment, DRC of PR107 showed a slight downward trend, but that of RY8-79, Ry7-33-97 and Tjir1 dropped obviously, especially for RY7-33-97. 3) Under the intensive tapping condition, the change rule of duration of latex flow was highly consistent with latex volume, and for these two parameters, RY8-79 was obviously higher than that of PR107, RY7-33-97 and Tjir1. After the ethrel treatment, the duration of latex flow and latex volume of all varieties were significantly increased (P<0.05), especially for PR107 and Tjir1. 4) The initial velocity of latex flow of RY8-79 and RY7-33-97 was obviously higher than that of PR107 and Tjir1 under intensive tapping condition, and etherl significantly reduced the initial velocity of all varieties. 5) The plugging index of RY8-79 was the lowest, while that of RY7-33-97 was the highest amongst the four varieties. Ethrel stimulation obviously reduced the plugging index. 6) Under the intensive tapping condition, the thiol content of RY8-79 was the highest, followed by PR107 and RY7-33-97, Tjir1 was the lowest. In about 48 h after ethrel stimulation, the thiols content of the four varieties generally decreased. Conclusion: The effects of latex flow induced by intensive tapping and ethrel stimulation are different among various varieties. The parameters of rubber particle size and DRC related with yield usually are difficult to recover to the healthy level under intensive tapping and ethrel overstimulation. Compared with other parameters, rubber particle size and DRC are less affected by the surrounding environment and can be used as references to evaluate the overflow. When the rubber particle size drops below 0.80 μm, it always indicates that the tapping intensity should be reduced. The varieties with fluent latex flow and high yield under natural conditions are usually intolerable to the intensive tapping and ethrel overstimulation, thus the varieties of RY8-79 and RY7-33-97 as main cultivars should avoid the application of ethrel while PR107 is fit to stimulate. Using young rubber tree as materials, combined with intensive tapping and ethrel stimulation, can be used as one of the methods for quickly screening the resistance of different varieties to tapping and ethrel stimulation in early breeding, and also provide a basis for the formulation of different variety tapping systems for rubber production.

Key words: rubber tree, intensive tapping, ethrel, rubber particle, latex flow

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