关键词: 橡胶树, cDNA-AFLP, 胁迫, 抗寒性, 分子育种

Abstract:

Rubber tree (Hevea brasiliensis) is the main source of nature rubber. Although rubber planting industry has become one of the important industries of Chinese agriculture in tropical areas, low temperature stress still restricts the development of rubber planting industry in China. The differential transcription of 11 cold-resistant rubber clones and 12 cold susceptible rubber clones was analyzed with and without cold stress by cDNA-AFLP. Through screening 100 primer sets, a total of 12 specific fragments were identified, cloned and sequenced, whose GenBank accession No. was GO269543-GO269554 respectively. By aligning with BLASTn and BLASTx, three fragments, TDF7-8, TDF5-15 and TDF16-10, exhibited the high homology with the resistance related gene ABC transport protein family, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DHAPS) and WRKY genes, respectively. All of the three fragments were up-regulated under cold stress. The other 9 fragments didn’t have high homologous sequences in GenBank. The results would be useful knowledge for rubber cold-resistant molecular breeding in future.

Key words: Hevea brasiliensis, cDNA-AFLP, abiotic stress, cold acclimation, molecular breeding