油茶炭疽病复合病菌鉴定及室内药剂评价

doi:10.11707/j.1001-7488.LYKX20220324

摘要/Abstract

摘要：

目的: 探讨油茶炭疽病复合病菌种类，筛选油茶炭疽病复合病菌的高效杀菌剂，为科学防治油茶炭疽病提供依据。方法: 从湖南、广西、广东、江苏，海南等地油茶成林采集炭疽病叶样本，采用组织分离法分离纯化致病菌，结合形态学和多基因联合系统发育分析进行种类鉴定；通过复合侵染试验研究致病菌之间的关系；采用菌丝生长速率法测定杀菌剂咪鲜胺、苯醚甲环唑、嘧菌酯、啶氧菌酯，氟吡菌酰胺和啶酰菌胺对油茶致病菌的室内毒力。结果: 1）从106个油茶炭疽病病斑中分离获得186株致病菌，其中主要病原菌是炭疽属真菌130株，拟盘多毛孢属真菌18株，新拟盘多毛孢属真菌28株，假拟盘多毛孢属真菌4株，链格孢属真菌6株；分离出单一炭疽病菌的病斑55个，而能同时分离出炭疽病菌和其他致病菌的病斑有51个，其中同一个病斑能同时分离出同属不同种或不同属致病菌的病斑有51个，分离的不同病菌有7种组合方式：同属不同种炭疽菌、炭疽菌和拟盘多毛孢属真菌、炭疽菌和新拟盘多毛孢属真菌、炭疽菌和假拟盘多毛孢属真菌、炭疽菌和链格孢属真菌两两组合以及炭疽菌和拟盘多毛孢属和新拟盘多毛孢属、炭疽菌和拟盘多毛孢属和链格孢属真菌3种菌的组合。2）将炭疽菌属和拟盘多毛孢属，炭疽菌属和新拟盘多毛孢属，炭疽菌同属不同种这3种分离率最多的组合方式致病菌回接到油茶上，发现炭疽病菌和其他致病菌复合接种的病斑显著大于只接同种病菌的病斑，表明油茶炭疽病斑中存在炭疽病菌与其他病原菌复合侵染协同增效的现象。3）室内毒力测定结果显示，苯醚甲环唑和咪鲜胺对炭疽菌属，拟盘多毛孢和新拟盘多毛孢致病菌均具有较强的抑制作用，平均EC₅₀在0.011~0.555 μg·mL^?1之间；其次是嘧菌酯和啶氧菌酯，这2种药剂对拟盘多毛孢和新拟盘多毛孢的室内毒力最强，平均EC₅₀在0.007~0.020 μg·mL^?1之间，但对炭疽菌的抑制作用较差；而啶酰菌胺和氟吡菌酰胺对油茶炭疽菌和拟盘多毛孢属致病菌几乎没有抑制作用。结论: 油茶炭疽病斑中存在炭疽菌和其他病菌复合侵染现象，咪鲜胺和苯醚甲环唑可作为防治油茶炭疽病复合侵染的主选药剂。

关键词: 油茶, 炭疽病, 致病菌, 复合侵染, 药剂筛选

Abstract:

Objective: This study aims to identify the compound pathogens causing anthracnose of Camellia oleifera and to screen effective fungicides for compound pathogens on C. oleifera, in order to provide targeted strategy for controlling C. oleifera anthracnose. Method: Leaf samples with anthracnose symptoms were collected from C. oleifera plantations in Hunan, Guangxi, Guangdong, Jiangsu and Hainan Provinces, China. Tissue separation method was used to isolate and purify the pathogenic fungi, and the morphological characteristics combined with and multi gene phylogenetic analysis were used to identify species. The composite infection experiments were used to study the relationship between pathogenic fungi and pathogenicity. The mycelial growth rate method was used to determine the indoor virulence of fungicides such as prochloraz, difenoconazole, azoxystrobin, picoxystrobin, fluopyram and boscalid to pathogens of C. oleifera. Result: 1）The result showed that a total of 186 isolates were obtained from 106 symptomatic lesions, among which 130 isolates of Colletotrichum genus were the main pathogens, followed by 18 isolates in Pestalotiopsis genus, 28 isolates in Neopestalotiopsis genus, 4 isolates in Pseudopestalotiopsis genus and 6 isolates in Alternaria genus. There were 55 lesions from which only species of Colletotrichum genus were isolated, and 51 lesions from which both Colletotrichum species and other pathogens were isolated. There were 51 lesions from which multiple pathogens with different species in the same genus or from the different genus were simultaneously isolated. There were 7 combinations of different strains isolated: different species of Colletotrichum genus, Colletotrichum sp. and Pestalotiopsis sp. or Neopestalotiopsis sp. or Pseudopestalotiopsis sp. or Alternaria sp., Colletotrichum sp., Pestalotiopsis sp. and Neopestalotiopsis sp. or Alternaria sp. 2）Further co-infection experiment showed that the lesion size caused by inoculation with mixed strains was significantly larger than that caused by inoculation with single strain, indicating that there was synergistic effect of compound infection of Colletotrichum sp. and other pathogens in C. oleifera anthracnose lesions. 3）The virulence test results showed that difenoconazole and prochloraz had the strongest inhibitory effects on Colletotrichum, Pestalotiopsis and Neopestalotiopsis pathogens of C. oleifera, with EC₅₀ mean values of 0.011 to 0.555 μg·mL^?1. Azoxystrobin and picoxystrobin had the highest indoor toxicity to Pestalotiopsis and Neopestalotiopsis, with an average EC₅₀ from 0.007 to 0.020 μg·mL^?1, but had weak inhibitory effect on Colletotrichum. Boscalid and fluopyram had almost no inhibitory effects. Conclusion: The results indicate that there is compound infection of Colletotrichum sp. and other pathogens in anthracnose lesions of C. oleifera, and prochloraz and difenoconazole can be used as the main fungicides to control C. oleifera anthracnose caused by compound infection of pathogens.

Key words: Camellia oleifera, anthracnose, pathogens, co-infection, fungicide selection

中图分类号:

S763.11

李玲玲,朱原野,张盛培,李河. 油茶炭疽病复合病菌鉴定及室内药剂评价[J]. 林业科学, 2023, 59(5): 100-108.

Lingling Li,Yuanye Zhu,Shengpei Zhang,He Li. Identification of the Compound Pathogens Causing Anthracnose of Camellia oleifera and Evaluation of Fungicides in Laboratory[J]. Scientia Silvae Sinicae, 2023, 59(5): 100-108.

图/表 5

表1

引物序列和PCR扩增程序"

基因 Gene	引物序列（5’-3’） Sequence	PCR扩增程序 PCR cycling conditions
核糖体转录间隔区 Internal transcribed spacers（ITS）	F: TCCGTAGGTGAACCTGCGG	95 ℃预变性5 min; 95 ℃变性30 s, 56 ℃退火30 s, 72 ℃延伸1 min, 32个循环; 72 ℃延伸10 min。 An initial denaturation at 95 ℃ for 5 min, followed by 32?cycles of denaturation at 95 ℃ for 30?s, annealing at 56 ℃ for 30?s and extension at 72 ℃ for 1 min, coupled with a final extension step of 10 min at 72 ℃.
核糖体转录间隔区 Internal transcribed spacers（ITS）	R: TCCTCCGCTTATTGATATGC
apn2和MAT1-2-1基因（ApMat）	F: TCATTCTACGTATGTGCCCG
apn2和MAT1-2-1基因（ApMat）	R: CCAGAAATACACCGAACTTGC
β-微管蛋白基因 β-tubulin（TUB2）	F: AACATGCGTGAGATTGTAAGT	95 ℃预变性8 min; 95 ℃变性30s, 55 ℃退火30 s, 72 ℃延伸1 min, 35个循环; 72 ℃延伸5 min。 An initial denaturation at 95 ℃ for 8 min, followed by 35?cycles of denaturation at 95 ℃ for 30?s, annealing at 55 ℃ for 30?s and extension at 72 ℃ for 1 min, coupled with a final extension step of 5 min at 72 ℃.
β-微管蛋白基因 β-tubulin（TUB2）	R: ACCCTCAGTGTAACCCTTGGC
延伸因子 Elongation factor-1α（EF-1α）	F: GTCGTYGTYATYGGHCAYGT	95 ℃预变性8 min; 95 ℃变性15 s, 55 ℃退火20 s, 72 ℃延伸1 min, 35个循环; 72 ℃延伸5 min。 An initial denaturation at 95 ℃ for 8 min, followed by 35?cycles of denaturation at 95 ℃ for 15?s, annealing at 55 ℃ for 20?s and extension at 72 ℃ for 1 min, coupled with a final extension step of 5 min at 72 ℃.
延伸因子 Elongation factor-1α（EF-1α）	R:ACHGTRCCRATACCACCRATCTT
组蛋白 Histone-3	F: ACTAAGCAGACCGCCCGCAGG	96 ℃预变性2 min; 96 ℃变性15 s, 55 ℃退火30 s, 72 ℃延伸35 s, 30个循环; 72 ℃延伸2 min。 An initial denaturation at 96 ℃ for 2 min, followed by 30?cycles of denaturation at 96 ℃ for 15?s, annealing at 55 ℃ for 30?s and extension at 72 ℃ for 35 s, coupled with a final extension step of 2 min at 72 ℃.
组蛋白 Histone-3	R: GCGGGCGAGCTGGATGTCCTT

表1

图1

图2

表2

表3

参考文献 0

	曹志华, 束庆龙, 程义明, 等. 12种农药对油茶炭疽菌的室内毒力测定. 农药, 2012, 51 (4): 304- 306. doi: 10.3969/j.issn.1006-0413.2012.04.024
	Cao Z H, Shu Q L, Cheng Y M, et al. Toxicity measurement of 12 fungicides on Colletotrichum gloeosporioides from Camellia oleifera . Agrochemicals, 2012, 51 (4): 304- 306. doi: 10.3969/j.issn.1006-0413.2012.04.024
	陈绍红, 孙　思, 王　军. 14种杀菌剂对油茶炭疽病的防治研究. 广东林业科技, 2017, 22 (2): 42- 45.
	Chen S H, Sun S, Wang J. Study on control of anthracnose of Camellia oleifera by 14 fungicides . Guangdong Forestry Science and Technology, 2017, 22 (2): 42- 45.
	陈永忠, 罗　建, 王　瑞, 等. 中国油茶产业发展的现状与前景. 粮食科技与经济, 2013, 38 (1): 10- 12. doi: 10.3969/j.issn.1007-1458.2013.01.035
	Chen Y Z, Luo J, Wang R, et al. Present situation and prospect of oil tea industry in China. Grain Science and Technology and Economy, 2013, 38 (1): 10- 12. doi: 10.3969/j.issn.1007-1458.2013.01.035
	冯宝珍, 李培谦. 月季黑斑病病原菌鉴定及室内药剂初步筛选. 植物保护学报, 2019, 46 (5): 1147- 1154. doi: 10.13802/j.cnki.zwbhxb.2019.2018149
	Feng B Z, Li P Q. Identification of the pathogen causing black spot of Chinese rose and fungicide screening for the disease control. Journal of Plant Protection, 2019, 46 (5): 1147- 1154. doi: 10.13802/j.cnki.zwbhxb.2019.2018149
	高杨杨. 2021. 琥珀酸脱氢酶抑制剂类杀菌剂对炭疽病菌的毒力差异机制. 泰安: 山东农业大学博士论文, 56-63.
	Gao Y Y. 2021. Molecular basis of selective activity of succinate dehydrogenase inhibitor fungicides against Colletotrichum spp. . Taian: PhD thesis of Shandong Agricultural University, 56-63. [ in Chinese]
	李　河, 李司政, 王悦辰, 等. 油茶苗圃炭疽病原菌鉴定及抗药性. 林业科学, 2019, 55 (5): 85- 94. doi: 10.11707/j.1001-7488.20190510
	Li H, Li S Z, Wang Y C, et al. Identification of the pathogens causing anthracnose of Camellia Oleifera in nursery and their resistance to fungicides . Scientia Silvae Sinicae, 2019, 55 (5): 85- 94. doi: 10.11707/j.1001-7488.20190510
	李　河, 李　杨, 蒋仕强, 等. 湖南省油茶炭疽病病原鉴定. 林业科学, 2017, 53 (8): 43- 53. doi: 10.11707/j.1001-7488.20170806
	Li H, Li Y, Jiang S Q, et al. Pathogen of oil-tea trees anthracnose caused by Colletotrichum spp . in Hunan Province. Scientia Silvae Sinicae, 2017, 53 (8): 43- 53. doi: 10.11707/j.1001-7488.20170806
	李　河, 周国英, 徐建平, 等. 一种油茶新炭疽病原的多基因系统发育分析鉴定. 植物保护学报, 2014, 41 (5): 602- 607. doi: 10.13802/j.cnki.zwbhxb.2014.05.034
	Li H, Zhou G Y, Xu J P, et al. Pathogen identification of a new anthracnose of Camellia oleifera in China based on multiple-gene phylogeny . Journal of Plant Protection, 2014, 41 (5): 602- 607. doi: 10.13802/j.cnki.zwbhxb.2014.05.034
	李　河, 周国英, 徐建平. 一种新油茶炭疽病原多基因序列鉴定. 植物保护, 2015, 41 (2): 92- 96. doi: 10.3969/j.issn.0529-1542.2015.02.016
	Li H, Zhou G Y, Xu J P. Pathogen identification of a new anthracnose of Camellia oleifera in China based on multiple-gene sequences . Plant Protection, 2015, 41 (2): 92- 96. doi: 10.3969/j.issn.0529-1542.2015.02.016
	李培琴, 明　洁, 张舒瑶, 等. 黄帝陵侧柏叶枯病的病原菌鉴定及防治药剂筛选. 西北农林科技大学学报(自然科学版), 2021, 49 (1): 74- 84. doi: 10.13207/j.cnki.jnwafu.2021.01.009
	Li P Q, Ming J, Zhang S Y, et al. Pathogen identification and selection of controlling fungicides of leaf blight of Platycladus orientalis at the Mausoleum of Yellow Emperor . Journal of Northwest A & F University (Natural Science Edition), 2021, 49 (1): 74- 84. doi: 10.13207/j.cnki.jnwafu.2021.01.009
	刘　霞, 杨克强, 朱玉凤, 等. 8种杀菌剂对核桃炭疽病病原菌胶孢炭疽菌的室内毒力. 农药学学报, 2013, 15 (4): 412- 420. doi: 10.3969/j.issn.1008-7303.2013.04.08
	Liu X, Yang K Q, Zhu Y F, et al. Laboratory toxicity of eight fungicides against Colletotrichum gloeosporioides causing walnut anthracnose . Chinese Journal of Pesticide Science, 2013, 15 (4): 412- 420. doi: 10.3969/j.issn.1008-7303.2013.04.08
	钱恒伟, 徐鹏程, 迟梦宇, 等. 尖孢镰刀菌与细极链格孢复合侵染引起甘薯茎枯病. 植物保护学报, 2017, 44 (5): 867- 868.
	Qian H W, Xu P C, Chi M Y, et al. Mixed infection by Fusarium oxysporum and Alternaria tenuissima on sweet potato Fusarium wilt . Journal of Plant Protection, 2017, 44 (5): 867- 868.
	王义勋, 刘　伟, 陈京元, 等. 不同杀菌剂对油茶炭疽病田间防治试验. 湖北林业科技, 2014, 43 (6): 35- 37. doi: 10.3969/j.issn.1004-3020.2014.06.011
	Wang Y X, Liu W, Chen J Y, et al. Fungicidal control of Colletotrichum gloeosporioides in field Camellia oleifera . Hubei Forestry Science and Technology, 2014, 43 (6): 35- 37. doi: 10.3969/j.issn.1004-3020.2014.06.011
	温　浩, 魏佳爽, 张桂军, 等. 九种杀菌剂对新拟盘多毛孢病菌的室内毒力作用. 农药学学报, 2019, 21 (4): 437- 443. doi: 10.16801/j.issn.1008-7303.2019.0071
	Wen H, Wei J S, Zhang G J, et al. Laboratory toxicity of nine fungicides against Neopestalotiopsis clavispora . Chinese Journal of Pesticide Science, 2019, 21 (4): 437- 443. doi: 10.16801/j.issn.1008-7303.2019.0071
	吴会杰, 严蕾艳, 郭　珍, 等. 黑点根腐病菌和镰孢菌复合侵染引起的甜瓜根腐病的鉴定. 中国瓜菜, 2021, 34 (10): 15- 19. doi: 10.3969/j.issn.1673-2871.2021.10.003
	Wu H J, Yan L Y, Guo Z, et al. Identification of melon root rot pathogen of Monosporascus cannonballus and Fusarium oxysporum f . sp. melonis. China Cucurbits and Vegetables, 2021, 34 (10): 15- 19. doi: 10.3969/j.issn.1673-2871.2021.10.003
	徐从英, 王　萌, 梁晓宇, 等. 云南西双版纳地区橡胶树炭疽病菌的系统进化分析和室内药剂筛选. 西南农业学报, 2021, 34 (12): 2653- 2658. doi: 10.16213/j.cnki.scjas.2021.12.014
	Xu C Y, Wang M, Liang X Y, et al. Phylogenetic analysis and fungicide screening for Colletotrichum causing rubber anthracnose in Xishuangbanna, Yunnan . Southwest China Journal of Agricultural Sciences, 2021, 34 (12): 2653- 2658. doi: 10.16213/j.cnki.scjas.2021.12.014
	徐娜娜, 庄治国, 孔　月, 等. 2021. 9种杀菌剂对牡丹炭疽病菌的效果评价. 现代农药, 20(5): 48−50.
	Xu N N, Zhuang Z G, Kong Y, et al. Effects of nine types of fungicides on mycelial growth of Colletotrichum gloeosporioides in peony. Modern Agrochemicals, Modern Agrochemicals, 20(5): 48−50.［in Chinese］
	严　明, 柏亚罗. 甲氧基丙烯酸酯类等四大类杀菌剂市场概况及前景展望. 现代农药, 2016, 15 (6): 1- 8. doi: 10.3969/j.issn.1671-5284.2016.06.001
	Yan M, Bai Y L. Market overview and prospect outlook on four fungicide sectors including methoxyacrylates. Modern Agrochemicals, 2016, 15 (6): 1- 8. doi: 10.3969/j.issn.1671-5284.2016.06.001
	张立伟, 王辽卫. 我国油茶产业的发展现状与展望. 中国油脂, 2021, 46 (6): 6- 9. doi: 10.19902/j.cnki.zgyz.1003-7969.2021.06.002
	Zhang L W, Wang L W. Prospect and development status of oil-tea camellia industry in China. China Oils and Fats, 2021, 46 (6): 6- 9. doi: 10.19902/j.cnki.zgyz.1003-7969.2021.06.002
	张永乐, 刘会香, 许永玉, 等. 围小丛壳菌山茶专化型与葡萄座腔菌复合侵染引致茶褐枯病. 茶叶科学, 2018, 38 (1): 87- 93. doi: 10.3969/j.issn.1000-369X.2018.01.009
	Zhang Y L, Liu H X, Xu Y Y, et al. The tea brown blight disease caused by co-infection of Glomerella cingulate f . sp. camellia and Botryosphaeria dothidea. Journal of Tea Science, 2018, 38 (1): 87- 93. doi: 10.3969/j.issn.1000-369X.2018.01.009
	赵丽玲, 钟　静, 施章吉, 等. 复合侵染水茄的两种菜豆金色花叶病毒属病毒基因组结构特征分析. 植物保护学报, 2020, 47 (2): 355- 364. doi: 10.13802/j.cnki.zwbhxb.2020.2019119
	Zhao L L, Zhong J, Shi Z J, et al. Characterization of the genome organization of two begomoviruses mixedly infecting wild eggplant Solanum torvum . Journal of Plant Protection, 2020, 47 (2): 355- 364. doi: 10.13802/j.cnki.zwbhxb.2020.2019119
	Cai L, Hyde K D, Taylor P W J, et al. A polyphasic approach for studying Colletotrichum . Fungal Diversity, 2009, 39 (1): 183- 204.
	Li H, Zhou G Y, Qi X Y, et al. First report of Colletotrichum henanense causing anthracnose on tea-oil trees in China . Plant Disease, 2018, 102 (5): 1040.
	Li J, Wang J C, Ding T B, et al. Synergistic Effects of a Tomato chlorosis virus and Tomato yellow leaf curl virus mixed infection on host tomato plants and the whitefly vector . Frontiers in plant science, 2021, 12, 672400. doi: 10.3389/fpls.2021.672400
	Li S Z, Li H. First report of Colletotrichum nymphaeae causing anthracnose on Camellia oleifera in China . Plant Disease, 2020, 104 (6): 1860.
	Maharachchikumbura S S N, Laringnonl P, Hyde K D, et al. Characterization of Neopestalotiopsis, Pestalotiopsis and Truncatella species associated with grapevine trunk diseases in France . Phytopathologia Mediterranea, 2016, 55, 380- 390.
	Ontiveros I, LopezMoya J J, DíazPendón J A. Co-infection of tomato plants with Tomato yellow leaf curl virus and Tomato chlorosis virus affects the interaction with host and whiteflies . Phytopathology, 2021, 112 (4): 944- 952.
	Woudenberg J H C, Groenewald J Z, Binder M, et al. Alternaria redefined . Studies in Mycology, 2013, 75 (1): 171- 212.
	Xu, J R, Hamer, J E. MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea . Genes & Development, 1996, 10 (21): 2696- 2706.

杀菌剂 Fungicide	质量浓度 Mass concentration/（μg·mL^?1）	不同病原种类的菌丝生长抑制率 Inhibition rate of different species（%）
杀菌剂 Fungicide	质量浓度 Mass concentration/（μg·mL^?1）	果生炭疽菌 C. fructicola	胶胞炭疽菌 C. gloeosporioides	暹罗炭疽菌 C. siamense	山茶炭疽菌 C. camelliae	新拟盘多毛孢属 Neopestalotiopsis sp.
啶酰菌胺 Boscalid	50	20.0±0.00b	17.0±0.00c	12.7±1.86d	15.6±0.72c	25.5±1.02a
氟吡菌酰胺 Fluopyram	50	33.0±1.06c	31.0±1.00d	25.3±0.29e	36.0±0.40b	43.3±0.96a
嘧菌酯 Azoxystrobin	50	57.4±0.57c	51.2±0.25d	40.5±0.69e	72.2±1.49b	100.0±0.00a
啶氧菌酯 Picoxystrobin	50	60.9±0.31d	64.1±1.05c	49.5±0.47e	86.8±0.49b	100.0±0.00a

杀菌剂 Fungicide	不同病原种类的 EC₅₀ Median effective concentration of different species/（μg·mL^?1）
杀菌剂 Fungicide	果生炭疽菌 C. fructicola	胶胞炭疽菌 C. gloeosporioides	暹罗炭疽菌 C. siamense	山茶炭疽菌 C. camelliae	新拟盘多毛孢属 Neopestalotiopsis sp.	拟盘多毛孢属 Pestalotiopsis sp.
嘧菌酯 Azoxystrobin	－	－	－	－	0.011±0.001	0.007±0.001
啶氧菌酯 Picoxystrobin	－	－	－	－	0.011±0.003	0.020±0.004
咪鲜胺 Prochloraz	0.043±0.006	0.023±0.001	0.022±0.002	0.011±0.009	0.110±0.040	0.026±0.007
苯醚甲环唑D ifenoconazole	0.306±0.023	0.228±0.012	0.153±0.007	0.123±0.039	0.555±0.145	0.150±0.044

[1]	张守科,李子坤,殷昊,张威,舒金平,王浩杰,周旭东,汪阳东. 油茶抗性无性系茶皂素含量对茶籽象幼虫肠道菌群结构的影响[J]. 林业科学, 2022, 58(7): 120-127.
[2]	李艳民,袁德义,叶天文,陈雅,韩春霞,肖诗鑫. 油茶种间杂交F₁代18个优良单株核型分析[J]. 林业科学, 2022, 58(4): 165-174.
[3]	李梓杨,陈晓琳,李丽丽,许诗萍,何苑皞. 油茶根系与内生细菌枯草芽孢杆菌互作早期的转录组分析[J]. 林业科学, 2022, 58(3): 48-58.
[4]	陈永忠,刘彩霞,许彦明,张震,彭映赫,陈隆升,苏以荣,王瑞,唐炜. 生草栽培对油茶林土壤微生物群落结构和稳定性的影响[J]. 林业科学, 2022, 58(11): 61-70.
[5]	陈星州,周国英,陈行钢,江玲玉,包安华,刘君昂. 油茶炭疽病菌果生刺盘孢效应子的筛选[J]. 林业科学, 2021, 57(9): 110-120.
[6]	李茜雅,张盛培,李河. 油茶果生刺盘孢液泡分选蛋白CfVps26的功能[J]. 林业科学, 2021, 57(8): 94-101.
[7]	叶天文,袁德义,李艳民,肖诗鑫,龚守富,张健,李素芳,罗健. 海南油茶的倍性鉴定[J]. 林业科学, 2021, 57(7): 61-69.
[8]	谢佰承,郭凌曜,杜东升,谭俨,王国栋. 油茶产量对关键生长时期热积温和高温日数的响应[J]. 林业科学, 2021, 57(5): 34-42.
[9]	陈新,王敏,傅茂润,王贵芳,相昆,刘庆忠,焦文晓,张美勇,许海峰. 核桃炭疽病发生相关的酚类物质代谢分析[J]. 林业科学, 2021, 57(10): 71-80.
[10]	高亚兰,何苑皋,李河. 调控油茶果生刺盘孢bZIP转录因子CfAp1的生物学功能[J]. 林业科学, 2020, 56(9): 30-39.
[11]	罗凡,陈志吉,蓝丽丽,杜孟浩,王超. 加热对油茶籽油及饼粕总酚及其抗氧化能力的影响[J]. 林业科学, 2020, 56(2): 61-68.
[12]	张守科,方林鑫,王毅,张威,舒金平,汪阳东,王浩杰. 基于组成型抗性性状的油茶抗茶籽象的评价模型[J]. 林业科学, 2020, 56(12): 67-74.
[13]	李河, 李司政, 王悦辰, 刘君昂, 徐建平, 周国英. 油茶苗圃炭疽病原菌鉴定及抗药性[J]. 林业科学, 2019, 55(5): 85-94.
[14]	李河, 李杨, 蒋仕强, 刘君昂, 周国英. 湖南省油茶炭疽病病原鉴定[J]. 林业科学, 2017, 53(8): 43-53.
[15]	金勤, 朱丹雪, 周国英, 李河, 何苑皞, 张茜. 绿色荧光蛋白标记枯草芽孢杆菌Y13^UV在油茶体内的定殖[J]. 林业科学, 2017, 53(7): 111-117.