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林业科学 ›› 2020, Vol. 56 ›› Issue (9): 51-57.doi: 10.11707/j.1001-7488.20200906

• 论文与研究报告 • 上一篇    下一篇

赤松纯林不同松枯梢病病级针叶的内生真菌多样性及群落结构

谢宪1,梁军1,2,*,朱彦鹏3,胡瑞瑞1,程元1,张星耀1,2   

  1. 1. 中国林业科学研究院森林生态环境与保护研究所 国家林业和草原局森林保护学重点实验室 北京 100091
    2. 昆嵛山森林生态系统国家定位观测研究站 烟台 264100
    3. 中国环境科学研究院 北京 100012
  • 收稿日期:2019-12-30 出版日期:2020-09-25 发布日期:2020-10-15
  • 通讯作者: 梁军
  • 基金资助:
    国家重点研发计划课题(2018YFC1200400);山东昆嵛山森林生态系统国家定位观测研究站运行补助(2019132127)

Diversity and Community Structure of Endophytic Fungi in the Pure Forest of Pinus densiflora Infected by Different Incidences of Sphaeropsis sapinea

Xian Xie1,Jun Liang1,2,*,Yanpeng Zhu3,Ruirui Hu1,Yuan Cheng1,Xingyao Zhang1,2   

  1. 1. Key Laboratory of Forest Protection of National Forestry and Grassland Administration Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry Beijing 100091
    2. Kunyushan Forest Ecosystem Research Station Yantai 264100
    3. Chinese Research Academy of Environmental Sciences Beijing 100012
  • Received:2019-12-30 Online:2020-09-25 Published:2020-10-15
  • Contact: Jun Liang

摘要:

目的: 研究赤松纯林中不同枯梢病病级的赤松针叶内生真菌多样性及群落结构,分析病—非病针叶内生真菌的多样性以及群落结构差异,为赤松纯林经营提供依据。方法: 采用高通量测序技术对真菌ITS1区进行测序,利用生物信息学方法分析赤松纯林中赤松无病斑和不同染病程度的针叶内生真菌的多样性和群落结构。结果: 在赤松纯林中,赤松无病斑针叶内生真菌的多样性高于染病针叶的内部真菌多样性;无病斑针叶中内生真菌的优势菌为多臂菌属和暗色球菌属,在枯梢病菌的侵染后群落结构发生改变;主坐标分析结果显示,无病斑针叶内生真菌与受侵染的针叶内真菌的群落构成差异显著;染病较轻的针叶与无病斑针叶相比,共生营养型真菌占比下降,病理营养型真菌占比增高,而腐生营养型真菌在全部针叶样本均占有较高的比例(37.15%~65.87%)。结论: 在赤松纯林中,赤松针叶内生真菌的多样性及群落结构受到枯梢病侵染的影响,无病斑针叶与染病针叶内生真菌群落结构差异显著。真菌营养型分类证实腐生营养型真菌和共生营养型真菌在无病斑针叶中为主要真菌,而在染病较轻针叶中以病理营养型真菌为主。

关键词: 赤松, 松枯梢病, 内生真菌, 群落结构

Abstract:

Objective: The diversity and community structure of endophytic fungi in the needles of Pinus densiflora with different incidences of Sphaeropsis sapinea were studied, and the differences of endophytic fungal diversity and community structure was analyzed, so as to provide basis for the management of pure forests of P. densiflora. Method: High-throughput sequencing technology was used to sequence the ITS1 region of the fungal rRNA gene, and further the diversity and community structure of endophytic fungi in different level of disease of P. densiflora were analyzed by using bioinformatics technology. Result: In the pure pine stands, the diversity of endophytic fungi in the asymptomatic needles of P. densiflora was higher than that of infected needles. The dominant fungi in the asymptomatic needles were Trichomerium and Phaeococomyces. The community structure changed after the infection of the pathogens. The principal coordinate analysis showed that the clustering distance within the asymptomatic endophytic fungi or the infected endophytic fungi was closer than that between the asymptomatic and the infected endophytic fungi. Compared with the asymptomatic needles, the proportion of symbiotroph fungi decreased, and the proportion of pathotrophic fungi was increased, and the proportion of saprotroph fungi accounted for a higher proportion (37.15% -65.87%) in all needles. Conclusion: In pure pine forest, the diversity and community structure of the endophytic fungi of P. densiflora are affected by S. sapinea, and the difference in the structure of endemic fungi between symptomatic needles and asymptomatic needles is significant. Fungal trophic classification confirms that saprotroph fungi and symbiotroph fungi are the main fungi in asymptomatic conifers, while pathotroph fungi are the dominant in lighter infected needles.

Key words: Pinus densiflora, Sphaeropsis sapinea, endophytic fungi, community structure

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