林业科学 ›› 2020, Vol. 56 ›› Issue (4): 109-120.doi: 10.11707/j.1001-7488.20200412
刘昌霖,周国英,肖柏,刘君昂
收稿日期:
2019-03-14
出版日期:
2020-04-25
发布日期:
2020-05-29
基金资助:
Changlin Liu,Guoying Zhou,Bai Xiao,Jun Liu
Received:
2019-03-14
Online:
2020-04-25
Published:
2020-05-29
摘要:
目的: 研究降香黄檀心材和边材中真菌群落结构和多样性及其与木材内环境因子的关系,为降香黄檀内生真菌价值评价和资源利用提供依据,为指导降香黄檀人工促进心材形成提供参考。方法: 采用高通量测序技术,结合木材理化性质,研究降香黄檀心材和边材内生真菌的群落结构和多样性,并采用方差分析、冗余分析等手段分析其与降香黄檀内环境因子的关系。结果: 经过高通量测序,共获得2 193 336条序列,碱基总数为761 438 850 bp,有效序列平均长度为385 bp。得到3 621个OTUs,分属26纲67目116科167属186种。纲水平上,心材内生真菌优势菌群为伞菌纲、粪壳菌纲和散囊菌纲,边材内生真菌优势菌群为粪壳菌纲、座囊菌纲、伞菌纲、地舌菌纲和银耳纲;属水平上,心材内生真菌优势菌群为暗枝顶孢壳属、镰刀菌属、孢子丝菌属、黑酵母样真菌属和球托霉属,边材内生真菌优势菌群为镰刀菌属、毛色二孢属、枝孢属、念珠菌属、德巴利酵母属和木霉属;种水平上,心材内生真菌优势菌群为孢子丝菌环境种、卵形孢球托霉菌和尖孢镰刀菌,边材内生真菌优势菌群为可可毛色二孢菌、酵母菌和镰刀菌。方差分析、冗余分析等结果表明,pH、灰分和钾元素与降香黄檀内生真菌属水平的群落结构呈负相关,热水抽提物、镁和钙元素与大部分属真菌群落呈正相关,与少量属真菌群落呈负相关。含水量、热水抽提物、矿质元素钙和镁与多样性指数Chaol、Shannon和Simpson指数呈正相关,pH、灰分和钾元素与其呈负相关。结论: 降香黄檀心材和边材含有丰富的内生真菌群落,群落结构丰度和多样性均为心材大于边材。引起心材和边材内生真菌群落结构性差异的主要木材内环境因子为pH、灰分和矿质元素钾,引起多样性差异的主要木材内环境因子为pH、含水率、矿质元素钙和钾,且含水平影响最大。
中图分类号:
刘昌霖,周国英,肖柏,刘君昂. 降香黄檀心材和边材内生真菌多样性[J]. 林业科学, 2020, 56(4): 109-120.
Changlin Liu,Guoying Zhou,Bai Xiao,Jun Liu. Diversity of Endophytic Fungi in Heartwood and Sapwood of Dalbergia odorifera[J]. Scientia Silvae Sinicae, 2020, 56(4): 109-120.
表3
木材理化与内生真菌多样性的相关性分析①"
丰度指数 Chao1 | 香侬指数 Shannon | 辛普森指数 Simpson | 含水率 Moisture content | pH | 灰分 Ash | 热水抽提物 Hot water extract | Ca | Mg | K | |
Chao1 | 1 | |||||||||
Shannon | 0.375 | 1 | ||||||||
Simpson | 0.321 | 0.996** | 1 | |||||||
MC | 0.449 | 0.966** | 0.970** | 1 | ||||||
pH | -0.339 | -0.985** | -0.993** | -0.965** | 1 | |||||
Ash | -0.089 | -0.851* | -0.844* | -0.734 | 0.839* | 1 | ||||
HWE | 0.046 | 0.889* | 0.925** | 0.876* | -0.930** | -0.725 | 1 | |||
Ca | 0.257 | 0.979** | 0.993** | 0.962** | -0.995** | -0.841* | 0.953** | 1 | ||
Mg | 0.121 | 0.916* | 0.938** | 0.930** | -0.928** | -0.820* | 0.910* | 0.956** | 1 | |
K | -0.286 | -0.987** | -0.997** | -0.966** | 0.997** | 0.842* | -0.944** | -0.999** | -0.948** | 1 |
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