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林业科学 ›› 2012, Vol. 48 ›› Issue (12): 134-140.doi: 10.11707/j.1001-7488.20121221

• 研究简报 • 上一篇    下一篇

一株绿僵菌的鉴定及其生物学特性

张亚波, 吴盼盼, 王鹏, 刘剑, 徐天森, 王浩杰, 舒金平   

  1. 中国林业科学研究院亚热带林业研究所 富阳 311400
  • 收稿日期:2011-12-17 修回日期:2012-03-01 出版日期:2012-12-25 发布日期:2012-12-25
  • 通讯作者: 舒金平

Identification and Biological Characteristics of A Metarhizium pingshaense Strain Isolated from Melanotus cribricollis Larva

Zhang Yabo, Wu Panpan, Wang Peng, Liu Jian, Xu Tiansen, Wang Haojie, Shu Jinping   

  1. Institute of Subtropical Forestry, CAF Fuyang 311400
  • Received:2011-12-17 Revised:2012-03-01 Online:2012-12-25 Published:2012-12-25

关键词: 平沙绿僵菌, EF- 1 &alpha, , 筛胸梳爪叩甲, 生物防治

Abstract:

An entomopathogenic fungal strain isolated from infected Melanotus cribricollis larvae was identified as Metarhizium pingshaense by both morphological and molecular methods. The 5' end of EF- 1 α of the isolate was sequenced, and submitted to GenBank with accession number JQ081264. Study on biological characteristics of the isolate showed that the optimum culture medium was PPDA. The range of optimum temperature for mycelium growth and conidia germination was from 25 ℃ to 30 ℃, respectively and the optimum temperature for conidia production was 25 ℃. The most suitable pH for conidia germination was 7.0. Conidia germination of this isolate could be obviously inhibited by ultraviolet radiation for less than 30 minutes.

Key words: Metarhizium pingshaense, EF- 1 &alpha, , Melanotus cribricollis, biological control

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