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林业科学 ›› 2006, Vol. 42 ›› Issue (5): 77-83.doi: 10.11707/j.1001-7488.20060514

• 论文及研究报告 • 上一篇    下一篇

光肩星天牛mtDNA COⅠ基因遗传差异的研究

安榆林1 杨晓军1 林晓佳2 师丽敏3 黄晓明4 陈建东1   

  1. 1.江苏出入境检验检疫局,南京210001;2.浙江出入境检验检疫局,杭州310012;3.南京林业大学,210037;4.东南大学,南京210009
  • 收稿日期:2005-06-20 修回日期:1900-01-01 出版日期:2006-05-25 发布日期:2006-05-25

Genetic Variation of mtDNA COⅠ in Anoplophora glabripennis(Coleoptera:Cerambycidae) Populations

An Yulin1,Yang Xiaojun1,Lin Xiaojia2,Shi Limin3,Huang Xiaoming4,Chen Jiandong1   

  1. 1.Plant Quarantine Laboratory of Jiangsu Entry-Exit Inspection and Quarantine Bureau of China Nanjing 210001;2. Plant Quarantine Laboratory of Zhejiang Entry-Exit Inspection and Quarantine Bureau of China Hangzhou 310012;3. Nanjing Forestry University Nanjing 210037; 4.Dongnan University Nanjing 210009
  • Received:2005-06-20 Revised:1900-01-01 Online:2006-05-25 Published:2006-05-25

摘要:

应用PCR直接测序法对不同地理来源的24个光肩星天牛、4个不同地理来源的黄斑星天牛及8个近缘种样品和花柳曲窄吉丁共37个样品的线粒体mtDNA COⅠ基因中一段504bp的序列进行测序。序列分析结果表明:光肩星天牛与其近缘种、外缘种间存在明显规律性差异;中国、美国、韩国等不同地理来源的光肩星天牛样品间存在一定的基因差异,并呈现出中国北方、中国南方、美国和韩国4个种群的特征,此研究结果不支持“美国的光肩星天牛是从中国传入的”的观点;光肩星天牛和黄斑星天牛样品间的差异较小,并未达到种级分类水平,应归为同1个种。

关键词: 光肩星天牛, 线粒体DNA, 细胞色素c氧化酶亚基Ⅰ基因, 遗传差异

Abstract:

504 bp fragment of mtDNA COⅠ gene of 37 individuals including 24 geographical populations of Anoplophora glabripennis, 4 geographical populations of Anoplophora nobilis, 8 sibling species and Agrilus marcopoli Obenberger were sequenced. The result indicated that there were evidently regular differences between Anoplophora glabripernnis populations and their sibling species. Sequence of samples from China, USA and Korea had their own sequence character, and reveals four populations among those samples: north China,south China,USA and Korea. Thus there weren't any evidence to prove that Anoplophora glabripernnis occurred in the USA were from China. Results of this study also showed that there were little different bases between Anoplophora glabripernnis and Anoplophora nobilis which strongly supports the findings that the two may in fact be a single species.

Key words: Anoplophora glabripernnis, mtDNA, COⅠ, Genetic variation