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林业科学 ›› 2008, Vol. 44 ›› Issue (1): 164-168.doi: 10.11707/j.1001-7488.20080126

• 论文 • 上一篇    下一篇

浙江马尾松毛虫不同地理种群间基因差异的ISSR分析

翁宏飚1 牛宝龙1 何丽华1 蒋平2 孟智启1   

  1. 1.浙江省农业科学院蚕桑研究所昆虫分子生物学实验室,杭州310021;2.浙江省林业有害生物防治检疫局,杭州310020
  • 收稿日期:2007-02-26 修回日期:1900-01-01 出版日期:2008-01-25 发布日期:2008-01-25

Genetic Diversity among the Dendrolimus punctatus Populations Sampled From Different Region of Zhejiang Province

Weng Hongbiao1,Niu Baolong1,He Lihua1,Jiang Ping2,Meng Zhiqi1   

  1. 1.Laboratory of Entomo-Molecular Biology,Zhejiang Academy of Agricultural Sciences Hangzhou 310021;2.Forest Pest Control and Quarantine Bureau of Zhejiang Province Hangzhou 310020
  • Received:2007-02-26 Revised:1900-01-01 Online:2008-01-25 Published:2008-01-25

关键词: 马尾松毛虫, ISSR, 遗传差异, 多态性

Abstract: Genetic diversity of Dendrolimus punctatus over five regions in Zhejiang Province were studied by ISSR-PCR.Thirteen primers were screened on the template of total DNA of the insects and total 74 bands of DNA fragments were amplified,of which the half(37 bands)was polymorphic.The genetic distance among the sampled populations ranged 0.114 4~0.475 4.The dendrogram was constructed based on their genetic distance using UPGMA method.The results indicated that there was low genetic diversity among the four populations from the main land,while the Dongtou population sampled from Dongtou island showed higher genetic diversity.

Key words: Dendrolimus punctatus, ISSR, genetic diversity, polymorphic