关键词: 日本落叶松, 体细胞胚胎发生, 培养基, 胚性愈伤组织, 成熟体细胞胚

Abstract:

Embryogenic cultures were initiated from immature zygotic embryos of Larix kaempferi on S culture medium containing 6-BA 0.5 mg·L^-1 , KT 0.5 mg·L^-1 and 2,4-D 1.0 mg·L^-1 .Embryogenic callus were subcultured and multiplicated on S culture medium containing dropping off each hormone concentration, 6-BA 0.25 mg·L ^-1 , KT 0.25 mg·L^-1 and 2,4-D 0.1 mg·L^-1 .We set up 3 steady-going embryogenic cell lines, such as ‘riyong10',‘riyong 69',‘ricao 34'.Somatic embryos obtained on S culture medium with ABA 15 mg·L ^-1 and PEG₄₀₀₀ 100 g ·L^-1 . These three culture media were added glutamine 450 mg·L^-1 , casein hydrolysate 500 mg·L ^-1 , sucrose 30 g·L^-1 and gellan gum 3 g·L^-1 (Sigma ),pH5.8.Mature somatic embryos can germinate and develop further into plantlets when they are isolated and cultured on a hormone-free S culture medium adding sucrose 25 g·L^-1 and gellan gum 3 g·L^-1 (Sigma^# ),pH5.8.

Key words: Larix kaempferi, somatic embryogenesis, culture medium, embryogenic callus, mature somatic embryos