油松成熟胚培养不定芽状态对其生根状况的影响

doi:10.11707/j.1001-7488.20140519

摘要/Abstract

关键词: 油松, 成熟胚, 改良1/2MS培养基, 不定芽诱导, 不定芽生根

Abstract:

The mature embryo of Pinus tabulaeformis was used as explants to induce adventitious buds and root on modified 1/2MS medium. The results showed that the largest multiplication rate with strong buds was obtained when adventitious bud cluster was divided into three pieces. The rooting rate of adventitious buds was 91.7%. The adventitious bud clusters with diameter larger than 0.9 cm, and the shoots with length longer than 6 mm had higher rooting rate. In the process of shoot elongation and multiplication, short culture duration could lead to a higher rooting capacity of the adventitious bud cluster, and the 28-days culture period was optimal for rooting, leading to a rooting rate of 73.3%. Adventitious bud clusters with big biomass were easily to root, and the higher the rooting rate the bigger the bud cluster biomass.

Key words: Pinus tabulaeformis, mature embryo, modified 1/2MS medium, adventitious bud induction, adventitious bud rooting

中图分类号:

S723.1⁺3

买凯乐, 邵砾群, 李荣珍, 董丽芬, 兰健花. 油松成熟胚培养不定芽状态对其生根状况的影响[J]. 林业科学, 2014, 50(5): 147-153.

Mai Kaile, Shao Liqun, Li Rongzhen, Dong Lifen, Lan Jianhua. Effect of the Status of Adventitious Buds In Vitro Cultured from Mature Embryo of Pinus tabulaeformis on Its Rooting[J]. Scientia Silvae Sinicae, 2014, 50(5): 147-153.

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