欢迎访问林业科学,今天是

林业科学 ›› 1998, Vol. 34 ›› Issue (6): 99-104.

• 研究简报 • 上一篇    下一篇

741杨器官、体细胞胚胎发生的细胞组织学及生理学的研究

杜克久,郑均宝,徐振华,李际泉,王进茂   

  1. 河北农业大学 保定 071000
  • 收稿日期:1997-01-31 出版日期:1998-11-25 发布日期:1998-11-25
  • 基金资助:
    河北省自然科学基金资助项目

STUDIES ON HISTOLOGY AND PHYSIOLOGY OF THE ORGANGENESIS AND SOMATIC EMBRYOGENESIS OF POPULUS ALBA × (P. DAVIDIANA × P. SIMONII) × P. TOMENTOSA IN VITRO

Kejiu Du,Junbao Zheng,Zhenhua Xu,Jiquan Li,Jimmao Wang   

  1. Hebei Agricultural University Baoding 071000
  • Received:1997-01-31 Online:1998-11-25 Published:1998-11-25
  • Supported by:
    河北省自然科学基金资助项目

关键词: 741杨, 体细胞胚胎, 酯酶同功酶, 可溶性蛋白

Abstract:

The yellow green hard callus is the best embryogenic callus, which obtained from the stem tip of Populus alba ×(P. davidiana ×P. simonii) ×P. tomentosa on the induced medium (MS+KT0. 1mg·L-1 +2, 4-D 0.05mg·L-1) after 10 days culture. Its formation frequeny of adventure buds, adventure roots and somatic embryos ane 100 %、19.4 % and 13.3 % respectively on the differentiated medium (MS + KT 0.3mg·L-1). Org ans and somatic embryos are all derived from the surface layer of calli. The somatic embryogenesis is very similar with the zygote embryogenesis. The esterase isozymes and soluble protein bands of various calli are very different.

Key words: Populus alba × (P. davidiana × P. simonii) × P. tomentosa, Stem tip, Somatic embryo esterase isozyme, Soluble protein