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林业科学 ›› 2000, Vol. 36 ›› Issue (zk): 51-55.doi: 10.11707/j.1001-7488.2000S107

• 论文及研究报告 • 上一篇    下一篇

湿地松抗病种子园的遗传多样性分析

易能君 韩正敏 尹佟明 黄敏仁 曹汉洋   

  1. 南京林业大学林木遗传与基因工程国家林业局重点实验室,南京210037;福建沙县官庄林场,沙县366500
  • 收稿日期:1998-05-04 修回日期:1900-01-01 出版日期:2001-01-25 发布日期:2001-01-25

GENETIC VARIATION OF RAPD MARKERS IN A DISEASE RESISTANT SEED ORCHARD OF PINUS ELLIOTTII ENGELM

Yi Nengjun,Hang Zhengmin,Yin Tongming,Huang Minren,Cao Hanyang   

  1. Forest Genetics and Gene Engineering Lab, Nanjing Forestry University Nanjing210037;Fujian Guanzuang Forest Farm Sha county366500
  • Received:1998-05-04 Revised:1900-01-01 Online:2001-01-25 Published:2001-01-25

摘要:

本文利用RAPD分子标记从DNA水平上研究了湿地松(Pinus elliottii Engelm)种子园的遗传多样性和连锁不平衡状况。结果表明,有效等位基因数目在各位点间变动范围为1.047~1.996 ,平均为1.4499;基因多样度范围为0.0449~0.499,平均为0.2781 ;Shannon信息指数的范围为0.1095~0.6922 ,平均为0.4327;这3个遗传多样性度量的大小顺序基本相同。104个分离位点共有5356个位点对,其中355对在显著性水平为0.05下达到显著的连锁不平衡状态。该抗病种子园具有较高的遗传多样性和一定程度的连锁不平衡。

关键词: 湿地松, 种子园, RAPD, 遗传多样性, 连锁不平衡

Abstract:

Genetic variation and linkage disequilibrium in a disease resistant seed orchard of Pinus elliottii Engelm were investigated by using RAPD markers in this paper. The results showed that the effective number of alleles per locus ranged between 1.047 and 1.996, averaging 1.449; Estimates of gene diversity per locus ranged between 0.0449 and 0.499, averaging 0.2781;and the Shannon's information index ranged between 0.1095 and 0.6922, averaging 0.4327. The order of these three measures per locus was the same. Out of 5356 pairs of RAPD loci in 104 segragating loci, 355 pairs showed significant deviations from linkage equilibrium. These results indicated that high genetic variation and linkage disequilibrium were harbored in this seed orchard.

Key words: Pinus elliottii Engelm, Seed orchard, RAPD markers, Genetic diversity, Linkage disequilibrium