云杉体细胞胚萌发前的干化标记

doi:10.11707/j.1001-7488.20140705

林业科学 ›› 2014, Vol. 50 ›› Issue (7): 31-36.doi: 10.11707/j.1001-7488.20140705

云杉体细胞胚萌发前的干化标记

张建伟, 王军辉, 张守攻, 李青粉, 夏燕

中国林业科学研究院林业研究所国家林业局林木培育重点实验室北京 100091

收稿日期:2013-09-11 修回日期:2013-11-19 出版日期:2014-07-25 发布日期:2014-07-04
基金资助:
“十二五”国家科技支撑项目“北方针叶树种高世代育种技术研究与示范”（2012BAD01B01）。

A Desiccation Indicator before Germination of Picea asperata Somatic Embryos

Zhang Jianwei, Wang Junhui, Zhang Shougong, Li Qingfen, Xia Yan

Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry, CAF Beijing 100091

Received:2013-09-11 Revised:2013-11-19 Online:2014-07-25 Published:2014-07-04
Contact: 王军辉

摘要/Abstract

摘要：

针对云杉体细胞胚萌发率极低和萌发质量不高的问题，以高同步化体细胞胚为材料，对干化方式、干化时间和干化普遍性等影响干化处理的因素进行探讨，并建立基于形态和颜色识别的云杉体细胞胚的干化标记。结果表明：采用“滤纸容器法”的干化方式更适用于云杉体细胞胚的干化处理；干化处理2周后能够获得最高的萌发率；本试验提供的干化处理方案对萌发促进作用具有普遍的适用性；以健壮的绿色子叶、胚轴及红色胚根为标记的体细胞胚能够普遍获得高的萌发率（平均83.63%）和较好的萌发质量。

关键词: 云杉, 体细胞胚, 萌发, 干化标记

Abstract:

In order to overcome the low germination rate and plantlet quality of Picea asperata, partial desiccation factors, such as drying approaches, drying time and universality, were investigated with highly synchronized somatic embryos, and a crucial partial desiccation indicator was established based on variation in the embryos morphology and color. The results indicated that the "paper vessel method" was more appropriate for the partial desiccation treatment, and the maximum germination rate was obtained with drying treatment for two weeks. Meanwhile, this study provided a drying treatment program which showed a general applicability for somatic embryos of P. asperata. The marked embryos of green cotyledon, hypocotyle and red radical could universally obtain high germination rate(average 83.63%)and good plantlet quality.

Key words: Picea asperata, somatic embryo, germination, partial desiccation indicator

中图分类号:

S718.46

张建伟, 王军辉, 张守攻, 李青粉, 夏燕. 云杉体细胞胚萌发前的干化标记[J]. 林业科学, 2014, 50(7): 31-36.

Zhang Jianwei, Wang Junhui, Zhang Shougong, Li Qingfen, Xia Yan. A Desiccation Indicator before Germination of Picea asperata Somatic Embryos[J]. Scientia Silvae Sinicae, 2014, 50(7): 31-36.

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云杉体细胞胚萌发前的干化标记

A Desiccation Indicator before Germination of Picea asperata Somatic Embryos

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