外源NO对低温胁迫下枇杷幼果抗氧化能力的影响

doi:10.11707/j.1001-7488.20100912

林业科学 ›› 2010, Vol. 46 ›› Issue (9): 73-78.doi: 10.11707/j.1001-7488.20100912

外源NO对低温胁迫下枇杷幼果抗氧化能力的影响

吴锦程¹，陈伟建²，蔡丽琴¹，谢翠萍¹，黄世杰¹，林良津¹，叶美兰¹

1.福建省莆田学院环境与生命科学系莆田 351100； 2.福建省莆田市农业检验监测中心莆田 351100

收稿日期:2009-08-04 修回日期:2010-01-25 出版日期:2010-09-25 发布日期:2010-09-25

Effects of Exogenous Nitric Oxide on Anti-Oxidation Capacities in Young Loquat Fruits under Low Temperature Stress

Wu Jincheng¹;Chen Weijian²;Cai Liqin¹;Xie Cuiping¹;Huang Shijie¹;Lin Liangjin¹;Ye Meilan¹

1.Environment and Life Science Department of Putian University Putian 351100; 2.Putian Agricultural Test and Inspection Centre of Fujian Province Putian 351100

Received:2009-08-04 Revised:2010-01-25 Online:2010-09-25 Published:2010-09-25

摘要/Abstract

摘要：

采用0.2,0.5,1.0 mmol·L^-1的一氧化氮(NO)供体硝普纳(SNP)处理3年生“早钟6号”枇杷幼苗后于-3 ℃人工气候室内进行低温胁迫,研究SNP处理对枇杷幼果丙二醛(MDA)、脯氨酸(Pro)、过氧化氢(H₂O₂)、AsA,DHA,GSH和GSSG的含量以及过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)和抗坏可血酸过氧化物酶(APX)活性的影响，探讨低温胁迫下外源NO对枇杷幼果抗寒性的调控机制。结果表明: SNP处理降低低温胁迫后枇杷幼果MDA和H₂O₂含量，不同程度上提高CAT,POD,SOD,APX活性和Pro含量，增加了AsA/DHA和GSH/GSSG比值。外源NO通过促进幼果保护酶活性的上升，降低膜质过氧化程度，增加渗透调节物质，可提高枇杷幼果的抗寒性，其中0.5 mmol·L^-1 SNP处理的效果较理想。

关键词: 一氧化氮, 枇杷幼果, 低温胁迫, 抗氧化能力

Abstract:

Three-year-old ‘Zaozhong No. 6’ loquat ( Eriobotrya japonica, Fujian Province) seedlings were foliar-sprayed with 0.2, 0.5 and 1.0 mmol·L^-1 of sodium nitroprusside (SNP, as NO donor). They were then subjected to low temperature (-3 ℃) stress in a phytotron. The effects of SNP on some physiological and biochemical indexes of young loquat fruits were studied. The indexes include malondialdehyde (MDA), proline (Pro), hydrogen peroxide (H₂O₂), ascorbic acid (AsA), dehydroascorbic acid (DHA), glutathione (GSH), oxidized glutathione (GSSG) content and the activities of catalase (CAT, EC 1.11.1.6), peroxidase (POD, EC 1.11.1.7), superoxide dismutase (SOD, EC 1.15.1.1) and ascorbate peroxidase (APX, EC 1.11.1.11). This study was to explore the regulation mechanism of exogenous nitric oxide (NO) on cold resistance of young loquat fruits under low temperature stress. The results showed that SNP treatment decreased levels of MDA and H₂O₂ in young loquat fruits after low temperature stress. Moreover, activities of CAT, POD, SOD and APX and Pro concentration of Pro were enhanced to some extent. Ratios of AsA/DHA and GSH/GSSG were increased, respectively. It is concluded that the exogenous NO could enhance the protective enzyme activity and reduce membrane lipid peroxidation, and increase the osmoregulation substance content of young loquat fruits after low temperature stress, resulting in an increase of the cold temperature resistance of young loquat fruits. In this study, we found that the desirable concentration of SNP was 0.5 mmol·L^{- 1}.

Key words: nitric oxide (NO), young loquat fruit, low temperature stress, anti-oxidation capacity

吴锦程;陈伟建;蔡丽琴;谢翠萍;黄世杰;林良津;叶美兰. 外源NO对低温胁迫下枇杷幼果抗氧化能力的影响[J]. 林业科学, 2010, 46(9): 73-78.

Wu Jincheng;Chen Weijian;Cai Liqin;Xie Cuiping;Huang Shijie;Lin Liangjin;Ye Meilan. Effects of Exogenous Nitric Oxide on Anti-Oxidation Capacities in Young Loquat Fruits under Low Temperature Stress[J]. Scientia Silvae Sinicae, 2010, 46(9): 73-78.

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外源NO对低温胁迫下枇杷幼果抗氧化能力的影响

Effects of Exogenous Nitric Oxide on Anti-Oxidation Capacities in Young Loquat Fruits under Low Temperature Stress

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